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Characterization of LF9, an octoploid strawberry genotype selected for rapid regeneration and transformation

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Abstract

Cultivated strawberry (Fragaria ×ananassa) is a valuable crop, yet the absence of a rapid, high-throughput transgenic system has precluded meaningful application of biotechnology and translation of information from plant models to this crop. A new octoploid strawberry genetic line Laboratory Festival #9 has been identified, selected solely for its rapid regeneration and efficient transformation. Direct organogenesis has been achieved from all tissues tested, with rapidly-growing shoot initials visible in as few as 13 days. The conditions for optimal shoot regeneration, transformant selection, root generation, and plant acclimatization are presented. The progression from explant to plant in soil can be achieved in about 60 days. The development of transformation protocols in this rapid-cycling genotype allows high-throughput studies of gene function in the octoploid strawberry genetic background.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

BA:

N6-benzyladenine

TDZ:

1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea (also thidiazuron)

IBA:

Indole butyric acid

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Acknowledgments

The authors gratefully recognize Dawn Bies and Holly Loucas for technical assistance and advice, and Dr. Prakash Lakshmanan for critical assessment of this manuscript. This work was supported by funding from the University of Florida Strawberry Breeding Program (PJS and CKC) and startup funding from the University of Florida Horticultural Sciences Department (KMF).

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Authors and Affiliations

  1. Plant Molecular and Cellular Biology Program and Horticultural Sciences Department, University of Florida, 1301 Fifield Hall, PO Box 110690, Gainesville, FL, 32611, USA

    Kevin M. Folta, Amit Dhingra, Leighan Howard & Philip J. Stewart

  2. Gulf Coast Research and Education Center, University of Florida, Wimauma, FL, 33598, USA

    Craig K. Chandler

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  1. Kevin M. Folta
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  2. Amit Dhingra
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  3. Leighan Howard
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  4. Philip J. Stewart
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  5. Craig K. Chandler
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Correspondence to Kevin M. Folta.

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Folta, K.M., Dhingra, A., Howard, L. et al. Characterization of LF9, an octoploid strawberry genotype selected for rapid regeneration and transformation. Planta 224, 1058–1067 (2006). https://doi.org/10.1007/s00425-006-0278-0

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  • DOI: https://doi.org/10.1007/s00425-006-0278-0

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