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The effects of salinity, crassulacean acid metabolism and plant age on the carbon isotope composition of Mesembryanthemum crystallinum L., a halophytic C3-CAM species

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Abstract

The carbon isotope composition of the halophyte Mesembryanthemum crystallinum L. (Aizoaceae) changes when plants are exposed to environmental stress and when they shift from C3 to crassulacean acid metabolism (CAM). We examined the coupling between carbon isotope composition and photosynthetic pathway by subjecting plants of different ages to salinity and humidity treatments. Whole shoot δ13C values became less negative in plants that were exposed to 400 mM NaCl in the hydroponic solution. The isotopic change had two components: a direct NaCl effect that was greatest in plants still operating in the C3 mode and decreased proportionally with increasing levels of dark fixation, and a second component related to the degree of CAM expression. Ignoring the presumably diffusion-related NaCl effect on carbon isotope ratios results in an overestimation of nocturnal CO2 gain in comparison to an isotope versus nocturnal CO2 gain calibration established previously for C3 and CAM species grown under well-watered conditions. It is widely taken for granted that the shift to CAM in M. crystallinum is partially under developmental control and that CAM is inevitably expressed in mature plants. Plants, cultivated under non-saline conditions and high relative humidity (RH) for up to 63 days, maintained diel CO2 gas-exchange patterns and δ13C values typical of C3 plants. However, a weak CAM gas-exchange pattern and an increase in δ13C value were observed in non-salt-treated plants grown at reduced RH. These observations are consistent with environmental control rather than developmental control of the induction of CAM in mature M. crystallinum under non-saline conditions.

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Abbreviations

CAM:

Crassulacean acid metabolism

PEPC:

Phosphoenolpyruvate carboxylase

PFD:

Photon flux density (400–700 nm)

RH:

Relative humidity

Rubisco:

Ribulosebisphosphate carboxylase/oxygenase

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Acknowledgements

This research was funded by the Andrew W. Mellon Foundation and the Smithsonian Tropical Research Institute. J.A.M.H. was supported by Dr. R.G. Dunn, a Queensland-Smithsonian Fellowship and the James Cook University Special Studies Programme. δ13C measurements were performed by Tom Maddox, Analytical Chemistry Laboratory, Institute of Ecology, University of Georgia, Athens, Georgia; plant carbon contents were measured by Elfriede Reisberg in the laboratory of Professor W. Kaiser, University of Würzburg, Germany.

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  1. Smithsonian Tropical Research Institute, P.O. Box 2072, Balboa, Ancon, Republic of Panama

    Klaus Winter

  2. Tropical Plant Sciences, James Cook University, Townsville, Queensland, 4811, Australia

    Joseph A. M. Holtum

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  1. Klaus Winter
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  2. Joseph A. M. Holtum
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Correspondence to Klaus Winter.

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Winter, K., Holtum, J.A.M. The effects of salinity, crassulacean acid metabolism and plant age on the carbon isotope composition of Mesembryanthemum crystallinum L., a halophytic C3-CAM species. Planta 222, 201–209 (2005). https://doi.org/10.1007/s00425-005-1516-6

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