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Expression analyses of Arabidopsis oligopeptide transporters during seed germination, vegetative growth and reproduction

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Abstract

AtOPT promoter-GUS fusions were constructed for six of the nine known, putative oligopeptide transporters (OPTs) in Arabidopsis thaliana and used to examine AtOPT expression at various stages of plant development. AtOPT1, AtOPT3, AtOPT4, AtOPT6 and AtOPT7 were expressed in the embryonic cotyledons prior to root radicle emergence. Except for AtOPT8, which gave weak expression, all AtOPTs were strongly expressed in post-germinative seedlings with strongest expression in vascular tissues of cotyledons and hypocotyls. Preferential expression of AtOPTs in vascular tissues was also observed in cotyledons, leaves, hypocotyls, roots, flowers, siliques, and seed funiculi of seedlings and adult plants. Differential tissue-specific expression was observed for specific AtOPTs. For example, AtOPT1, AtOPT3 and AtOPT8 were uniquely expressed in pollen. Only AtOPT1 was expressed in growing pollen tubes, while only AtOPT6 was observed in ovules. AtOPT8 was transiently expressed in seeds during early stages of embryogenesis. Iron limitation was found to enhance expression of AtOPT3. These data suggest distinct cellular roles for specific AtOPTs including nitrogen mobilization during germination and senescence, pollen tube growth, pollen and ovule development, seed formation and metal transport.

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Acknowledgements

We thank Dr. Elizabeth E. Rogers and Dr. Bruce A McClure for their valuable technical suggestions and helpful discussions. We also thank Dr. Tom Guilfoyle for giving us access to his spectrofluorometer. This work was supported in part by the University of Missouri-Columbia Life Sciences Post-Doctoral Fellowship (H.O.) and by the National Science Foundation (grant MCB-0235286 to G.S. and W.G.).

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  1. Hiroki Osawa

    Present address: Graduate School of Agriculture and Agricultural Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

Authors and Affiliations

  1. Division of Plant Sciences, The University of Missouri, Columbia, MO, 65211, USA

    Minviluz G. Stacey, Hiroki Osawa, Ami Patel, Walter Gassmann & Gary Stacey

  2. National Center for Soybean Biotechnology, Division of Biochemistry, The University of Missouri, Columbia, MO, 65211, USA

    Gary Stacey

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  1. Minviluz G. Stacey
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Correspondence to Gary Stacey.

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Stacey, M.G., Osawa, H., Patel, A. et al. Expression analyses of Arabidopsis oligopeptide transporters during seed germination, vegetative growth and reproduction. Planta 223, 291–305 (2006). https://doi.org/10.1007/s00425-005-0087-x

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  • DOI: https://doi.org/10.1007/s00425-005-0087-x

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