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Lack of mycorrhizal autoregulation and phytohormonal changes in the supernodulating soybean mutant nts1007

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Abstract

Autoregulatory mechanisms have been reported in the rhizobial and the mycorrhizal symbiosis. Autoregulation means that already existing nodules or an existing root colonization by an arbuscular mycorrhizal fungus systemically suppress subsequent nodule formation/root colonization in other parts of the root system. Mutants of some legumes lost their ability to autoregulate the nodule number and thus display a supernodulating phenotype. On studying the effect of pre-inoculation of one side of a split-root system with an arbuscular mycorrhizal fungus on subsequent mycorrhization in the second side of the split-root system of a wild-type soybean (Glycine max L.) cv. Bragg and its supernodulating mutant nts1007, we observed a clear suppressional effect in the wild-type, whereas further root colonization in the split-root system of the mutant nts1007 was not suppressed. These data strongly indicate that the mechanisms involved in supernodulation also affect mycorrhization and support the hypothesis that the autoregulation in the rhizobial and the mycorrhizal symbiosis is controlled in a similar manner. The accumulation patterns of the plant hormones IAA, ABA and Jasmonic acid (JA) in non-inoculated control plants and split-root systems of inoculated plants with one mycorrhizal side of the split-root system and one non-mycorrhizal side, indicate an involvement of IAA in the autoregulation of mycorrhization. Mycorrhizal colonization of soybeans also resulted in a strong induction of ABA and JA levels, but on the basis of our data the role of these two phytohormones in mycorrhizal autoregulation is questionable.

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Abbreviations

ABA:

Abscisic acid

AMF:

Arbuscular mycorrhizal fungus

IAA:

Indole-3-acetic

JA:

Jasmonic acid

min:

minute

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Acknowledgements

This work was supported by a research grant of the “Universität für Bodenkultur Wien” Austria to C. Meixner and a grant of the “Hochschuljubiläumsstiftung der Stadt Wien” Austria to H. Vierheilig.

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Authors and Affiliations

  1. Institut für Pflanzenschutz (DAPP), Universität für Bodenkultur Wien, Peter Jordan-Strasse 82, 1190, Wien, Austria

    Claudia Meixner & Horst Vierheilig

  2. Institut für Botanik, Technische Universität Dresden, Zellescher Weg 22, 01062, Dresden, Germany

    Jutta Ludwig-Müller

  3. Institute of Plant Biochemistry, Weinberg 3, 06120, Halle, Germany

    Otto Miersch

  4. ARC Centre of Excellence for Integrative Legume Research, University of Queensland, Brisbane, QLD, 4072, Australia

    Peter Gresshoff

  5. School of Life Sciences, SunYat-Sen (Zhongshan) University, Xingangxi Road 135, Guangzhou, 510275, People’s Republic of China

    Christian Staehelin

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  1. Claudia Meixner
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Correspondence to Horst Vierheilig.

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Meixner, C., Ludwig-Müller, J., Miersch, O. et al. Lack of mycorrhizal autoregulation and phytohormonal changes in the supernodulating soybean mutant nts1007 . Planta 222, 709–715 (2005). https://doi.org/10.1007/s00425-005-0003-4

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