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High salinity reduces the content of a highly abundant 23-kDa protein of the mangrove Bruguiera parviflora

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Abstract

A significant decrease in the amount of a protein, whose migration in two-dimensional gel electrophoresis corresponds to an apparent molecular mass of 23 kDa and pI=6.5, was observed in leaves of NaCl-treated Bruguiera parviflora (Roxb.) Wt. & Arn. ex Griff. seedlings. This particular salt-sensitive protein, designated as SSP-23, almost disappeared after 45 days of treatment in 400 mM NaCl as compared to untreated seedlings (0 mM NaCl) where the presence of the protein was significant. A polyclonal antibody raised against the 23-kDa protein was used to determine the subcellular localization of this protein in leaves by cross-reaction with proteins from isolated chloroplasts, mitochondria, peroxisomes and cytosol fractions on Western blots. SSP-23 was confirmed to be localized in the cytosol by immunoblotting. The disappearance of SSP-23 as a result of high NaCl treatment suggests that this protein is salt-sensitive and has a possible role in salt adaptation.

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Abbreviations

2-D :

Two dimensional

PAGE :

Polyacrylamide gel electrophoresis

SDS :

Sodium dodecyl sulfate

SSP :

Salt-sensitive protein

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Acknowledgements

The authors are grateful to Prof. P. Das, Director, Regional Plant Resource Centre, Bhubaneswar for providing laboratory facilities. The financial assistance from CSIR (Grant No. 38(983)/EMR-II), New Delhi, is duly acknowledged.

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Authors and Affiliations

  1. National Institute for Plant Biodiversity Conservation and Research, Nayapalli, 751015, Bhubaneswar, Orissa, India

    Asish Kumar Parida & Anath Bandhu Das

  2. Regional Plant Resource Centre, Nayapalli, 751015, Bhubaneswar, Orissa, India

    Anath Bandhu Das & Prasanna Mohanty

  3. Centre for Environmental Management of Degraded Ecosystems, School of Environmental Studies, North Campus, University of Delhi, 110 007, Delhi, India

    Bhabatosh Mittra

  4. Electron Microscopy Facility, All India Institute of Medical Sciences, 110019, New Delhi, India

    Taposh Kumar Das

Authors
  1. Asish Kumar Parida
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  2. Bhabatosh Mittra
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  3. Anath Bandhu Das
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  4. Taposh Kumar Das
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  5. Prasanna Mohanty
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Correspondence to Anath Bandhu Das.

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Parida, A.K., Mittra, B., Das, A.B. et al. High salinity reduces the content of a highly abundant 23-kDa protein of the mangrove Bruguiera parviflora. Planta 221, 135–140 (2005). https://doi.org/10.1007/s00425-004-1415-2

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