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Intracellular localization of VDAC proteins in plants

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Abstract

Voltage-dependent anion channels (VDACs) are porin-type β-barrel diffusion pores. They are prominent in the outer membrane of mitochondria and facilitate metabolite exchange between the organelle and the cytosol. Here we studied the subcellular distribution of a plant VDAC-like protein between plastids and mitochondria in green and non-green tissue. Using in vitro studies of dual-import into mitochondria and chloroplasts as well as transient expression of fluorescence-labeled polypeptides, it could be clearly demonstrated that this VDAC isoform targets exclusively to mitochondria and not to plastids. Our results support the idea that plastids evolved a concept of solute exchange with the cytosol different from that of mitochondria.

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Fig. 1a–d
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Fig. 5a,b

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Abbreviations

AOX:

Alternative oxidase

p:

Precursor form

POM36:

Putative outer mitochondrial membrane proteins of 36 kDa

SSU:

Small subunit of ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco)

VDAC:

Voltage-dependent anion channel

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Acknowledgement

We thank Dr. G. Jach (Cologne) for providing the ps-ds-Red clone, Dr. Engstler (Munich) and Dr. Lohr (Kaiserslautern) for introducing us to fluorescence microscopy, and Dr. J. Whelan for introducing us into the method of dual imports. This work was supported by the Deutsche Forschungsgemeinschaft and Fonds der Chemischen Industrie. C. Clausen and I. Ilkavets contributed equally to this work.

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Author notes

  1. Cathrin Clausen

    Present address: Biochemisches Institut, Universität Kiel, Olshausenstr. 40, 24098, Kiel, Germany

  2. Hrvoje Fulgosi

    Present address: Department of Molecular Genetics, Ruder Boskovic Institute, University of Zagreb, Bijenicka 54, 10000, Zagreb, Croatia

Authors and Affiliations

  1. Department Biologie I, Ludwig-Maximilians-Universität München, Menzingerstr. 67, 80638, München, Germany

    Cathrin Clausen, Iryna Ilkavets, Rowena Thomson, Katrin Philippar, Aleksandar Vojta, Hrvoje Fulgosi & Jürgen Soll

  2. Fachbereich Biologie, Universität Kaiserslautern, Erwin-Schrödinger-Straße, 67663, Kaiserslautern, Germany

    Torsten Möhlmann & Ekkehard Neuhaus

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  1. Cathrin Clausen
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Correspondence to Jürgen Soll.

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Clausen, C., Ilkavets, I., Thomson, R. et al. Intracellular localization of VDAC proteins in plants. Planta 220, 30–37 (2004). https://doi.org/10.1007/s00425-004-1325-3

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