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Expression of the patatin-related phospholipase A gene AtPLA IIA in Arabidopsis thaliana is up-regulated by salicylic acid, wounding, ethylene, and iron and phosphate deficiency

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Abstract

In Arabidopsis thaliana (L.) Heynh., the cytosolic, patatin-related phospholipase A enzymes comprise a family of ten genes designated AtPLAs thought to be involved in auxin and pathogen signalling [A. Holk et al. (2002) Plant Physiol 130:90–101]. One of these, AtPLA IIA, is investigated here by studying its transcriptional regulation through transgenic Arabidopsis plants containing the AtPLA IIA promoter (PIIA) fused to the β-glucuronidase (GUS) gene. GUS activity appeared in leaves at 10–12 days and became increasingly stronger with age in all leaves. From the same age on, strong GUS activity was visible in the basal stipules of the rosette leaves. PIIA-dependent GUS activity was found in the older parts of the primary root (from 10 days on) and, later in development, in older parts of side roots, and the root cap. No GUS activity was detected in flower organs. PIIA-dependent GUS expression in 12-day-old plants was up-regulated after treatment by salicylic acid, Bion, wounding, 1-aminocyclopropane-1-carboxylic acid (ACC) and jasmonic acid. When transgenic PIIA::uidA plants were grown devoid of iron, 9-day-old plants exhibited increased GUS activity in the leaves and, when devoid of phosphate, 11-day-old plants had increased GUS activity in the roots. In conclusion, this member of the patatin-related phospholipase A gene family showed properties of a defence and iron-stress and phosphate-stress gene, being transcriptionally up-regulated within hours or days.

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Fig. 1a–k
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Abbreviations

AACOCF 3 :

Arachidonyltrifluoromethylcarbon

ACC :

1-Aminocyclopropane-1-carboxylic acid

β-BODIPY-PC :

2-(4,4-Difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoyl)-1-hexadecanoyl-sn-glycero-3-phosphocholine

bis-BODIPY-PC :

1,2-Bis-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-undecanoyl)-sn-glycero-3-phosphocholine

ETYA :

5,8,11,14-Eicosatetraynoic acid

GUS :

β-Glucuronidase

HELSS :

Tetrahydro-3-(1-naphtalenylnaphthalenyl)-2H-pyran-2-one

JA :

Jasmonic acid

NDGA :

Nordihydroguajaretic acid

PACOCF 3 :

Palmitoyltrifluoromethyl ketone

PIIA :

AtPLA IIA promoter

PLA :

Phospholipase A

SA :

Salicylic acid

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Acknowledgements

This work was supported by the Deutsche Forschungsgemeinschaft (Sche 207/9-1), the Bundesministerium für Forschung (50WB0010), and the Deutsche Volkswagen Stiftung [A.Z.51.16-76251-9/97(ZN607)].

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  1. Institut für Zierpflanzenbau, Baumschule und Pflanzenzüchtung, Universität Hannover, Herrenhäuser Str. 2, 30419, Hannover, Germany

    Steffen Rietz, André Holk & Günther F. E. Scherer

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  1. Steffen Rietz
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  2. André Holk
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  3. Günther F. E. Scherer
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Correspondence to Günther F. E. Scherer.

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Rietz, S., Holk, A. & Scherer, G.F.E. Expression of the patatin-related phospholipase A gene AtPLA IIA in Arabidopsis thaliana is up-regulated by salicylic acid, wounding, ethylene, and iron and phosphate deficiency. Planta 219, 743–753 (2004). https://doi.org/10.1007/s00425-004-1275-9

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