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Molecular cloning and expression analysis of three genes encoding starch synthase II in rice

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Abstract

Three starch synthase (SS) genes, OsSSII-1, OsSSII-2 and OsSSII-3, were identified in rice (Oryza sativa L.) and localized to chromosomes 10, 2 and 6, respectively. The three OsSSII full-length cDNAs were cloned, and the predicted amino acid sequences were found to share 52–73% similarity with other members of the plant SSII family. The SS activity of each OsSSII was confirmed by expression and enzyme activity assay in Escherichia coli. Expression profile analysis revealed that OsSSII-1 was expressed in endosperms, leaves and roots; OsSSII-2 was mainly expressed in leaves, while OsSSII-3 was mainly expressed in endosperms. Similar to the OsSSI proteins, the OsSSII-2 and OsSSII-3 proteins were found in the soluble as well as the starch-granule-bound fractions in rice. The roles of the OsSSII proteins in starch biosynthesis in rice and the evolutionary relationships of the genes encoding monocotyledonous and dicotyledonous class-II SS enzymes are discussed.

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Abbreviations

CDS :

Coding domain sequence

EST :

Expressed sequence tag

GB:

Granule-bound

Glc:

Glucose

SS :

Starch synthase

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (grant No. 39830250) and the Natural Science Foundation of Zhejiang Province, China (grant No. ZA0106). H. Jiang and W. Dian contributed equally to this work.

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Authors and Affiliations

  1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, 310029, Hangzhou, P. R. China

    Huawu Jiang, Weimin Dian, Feiyang Liu & Ping Wu

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  1. Huawu Jiang
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  2. Weimin Dian
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  3. Feiyang Liu
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  4. Ping Wu
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Correspondence to Ping Wu.

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Jiang, H., Dian, W., Liu, F. et al. Molecular cloning and expression analysis of three genes encoding starch synthase II in rice. Planta 218, 1062–1070 (2004). https://doi.org/10.1007/s00425-003-1189-y

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