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Cloning of soybean genes induced during hypersensitive cell death caused by syringolide elicitor

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Abstract

Syringolide elicitors produced by bacteria expressing Pseudomonas syringae pv. glycinea avirulence gene D (avrD) induce hypersensitive cell death (HCD) only in soybean (Glycine max [L.] Merr.) plants carrying the Rpg4 disease resistance gene. Employing a differential display method, we isolated 13 gene fragments induced in cultured cells of a soybean cultivar Harosoy (Rpg4) treated with syringolides. Several genes for isolated fragments were induced by syringolides in an rpg4 cultivar Acme as well as in Harosoy; however, the genes for seven fragments designated as SIH (for syringolide-induced/HCD associated) were induced exclusively or strongly in Harosoy. cDNA clones for SIH genes were obtained from a cDNA library of Harosoy treated with syringolide. Several sequences are homologous to proteins associated with plant defense responses. The SIH genes did not respond to a non-specific β-glucan elicitor, which induces phytoalexin accumulation but not HCD, suggesting that the induction of the SIH genes is specific for the syringolide–Harosoy interaction. HCD and the induction of SIH genes by syringolides were independent of H2O2. On the other hand, Ca2+ was required for HCD and the induction of some SIH genes. These results suggest that the induction of SIH genes by syringolides could be activated through the syringolide-specific signaling pathway and the SIH gene products may play an important role(s) in the processes of HCD induced by syringolides.

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Abbreviations

AOS:

active oxygen species

CHS:

chalcone synthase

DPI:

diphenylene iodonium

HCD:

hypersensitive cell death

HR:

hypersensitive response

PAL:

phenylalanine ammonia lyase

SID:

syringolide-induced/defense associated

SIG:

syringolide-induced/general

SIH:

for syringolide-induced/HCD associated

XET:

xyloglucan endotransglycosylase

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Acknowledgements

We thank the late Dr. Noel T. Keen (University of California, Riverside) for encouraging us in this study. This work was supported by the Ministry of Education, Culture, Sports, Science and Technology of Japan (grant nos. 11760030 and 13660040).

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Author notes

  1. Takuya Hagihara

    Present address: Agricultural Research Institute, Hokuren Federation of Agricultural Cooperatives, Naganuma, 069-1316, Hokkaido, Japan

  2. Naoto Yamaoka

    Present address: Faculty of Agriculture, Ehime University, 790-8566, Matsuyama, Japan

Authors and Affiliations

  1. Division of Biological Sciences, Graduate School of Science, Hokkaido University, 060-0810, Sapporo, Japan

    Takuya Hagihara, Masaru Hashi, Yoji Takeuchi & Naoto Yamaoka

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  1. Takuya Hagihara
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  2. Masaru Hashi
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Correspondence to Naoto Yamaoka.

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Hagihara, T., Hashi, M., Takeuchi, Y. et al. Cloning of soybean genes induced during hypersensitive cell death caused by syringolide elicitor. Planta 218, 606–614 (2004). https://doi.org/10.1007/s00425-003-1136-y

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