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Seasonal variation in transcript accumulation in wood-forming tissues of maritime pine (Pinus pinaster Ait.) with emphasis on a cell wall glycine-rich protein

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Abstract

Wood formation is being increasingly studied at cellular and biochemical levels; however, gene expression and regulation during wood formation remain poorly understood. Up to six types of wood can be studied within the same tree (early wood, late wood, juvenile wood, mature wood, reaction wood and opposite wood). These six types are characterized by different chemical, physical and anatomical properties. Using the cDNA-amplified fragment length polymorphism (AFLP) technique, we screened several thousand cDNA fragments from differentiating xylem of maritime pine (Pinus pinaster Ait.) comparing early wood vs. late wood and compression wood vs. opposite wood after 8 or 120 days of bending. About 100 transcript-derived fragments (TDFs) showed qualitative or quantitative variations between these different samples. The relative abundance of these TDFs was subsequently analyzed by reverse Northern using RNA derived from early and late wood. Analysis of variance (ANOVA) was used to identify differentially expressed TDFs (P<0.01) and reverse transcription–polymerase chain reaction to confirm the differential expression of some TDFs. Among the genes with a known function, transcript expression and nucleotide sequence variation analysis showed a cell wall glycine-rich protein to be a strong candidate gene for wood properties.

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Abbreviations

ACC:

1-aminocyclopropane-1-carboxylic acid

AFLP:

amplified fragment length polymorphism

CW:

compression wood

EST:

expressed sequence tag

EW:

early wood

G3PDH:

glyceraldehyde-3-phosphate dehydrogenase

GRP:

glycine-rich protein

HSP:

heat-shock protein

INDEL:

insertion/deletion

LW:

late wood

OW:

opposite wood

SNP:

single nucleotide polymorphism

TDF:

transcript-derived fragment

VW:

vertical wood

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Acknowledgements

G.L.P. was supported by a joint fellowship from the Institut National de la Recherche Agronomique and the region Aquitaine. J.P. was supported by a doctoral fellowship from Fundação para a Ciência e a Tecnologia (SFRH/BD/3129/2000). This work has received funding from the European Union (Gemini project QLK 5 CT 1999-00942) and France (DERF No. 01.40.40/99). We thank Josquin Tibbits (Tree Development and Forest Biotechnology Laboratory, University of Melbourne) and Herman Höfte (INRA Versailles) for valuable comments and critical reading of the manuscript, and Annik Lacombe (UCDIST-Jouy) for proof reading and correction of the English.

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  1. 1 UMR Biogeco 1202, INRA, Equipe de génétique, 69 route d'Arcachon, 33612, Cestas, Cédex, France

    Grégoire Le Provost, Jorge Paiva, David Pot, Jean Brach & Christophe Plomion

  2. 2 Plant Cell Biotechnology Lab., IBET/ITQB, Quinta do Marquês, 2780, Oeiras, Portugal

    Jorge Paiva

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  1. Grégoire Le Provost
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Correspondence to Christophe Plomion.

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Le Provost, G., Paiva, J., Pot, D. et al. Seasonal variation in transcript accumulation in wood-forming tissues of maritime pine (Pinus pinaster Ait.) with emphasis on a cell wall glycine-rich protein. Planta 217, 820–830 (2003). https://doi.org/10.1007/s00425-003-1051-2

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