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Abiotic stresses affecting water balance induce phosphoenolpyruvate carboxylase expression in roots of wheat seedlings

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Abstract

Phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) plays an important role in CO2 fixation in C4 and CAM plants. In C3 plants, PEPC is widely expressed in most organs; however, its function is not yet clearly established. With the aim of providing clues on the function of PEPC in C3 plants, we have analyzed its pattern of expression in wheat (Triticum aestivum L.) seedlings. Roots showed almost double the level of PEPC activity of shoots. Further analysis of PEPC expression in roots by in situ localization techniques showed a high accumulation of PEPC transcripts and polypeptides in meristematic cells, whereas in the rest of the root PEPC localized preferentially to the vascular tissue. Treatment with NaCl and LiCl induced PEPC expression in roots. Similarly, other abiotic stresses affecting water status, such as drought or cold, induced PEPC expression. Induction was root-specific except for the cold treatment, which also induced PEPC in shoots, although to a lesser extent. In contrast, hypoxia, which does not affect water balance, did not promote any induction of PEPC expression. These results suggest an important role for this enzyme in the adaptation of plants to environmental changes.

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Abbreviations

Adh :

alcohol dehydrogenase gene

CAM:

crassulacean acid metabolism

PEPC:

phosphoenolpyruvate carboxylase

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Acknowledgements

This work was supported by grants BMC2001-2366 from Ministerio de Ciencia y Tecnología and CVI-182 from Junta de Andalucía, Spain. R.S. was supported by a pre-doctoral fellowship from MCyT, Spain.

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  1. Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas "Isla de la Cartuja", Avda Américo Vespucio s/n, 41092, Sevilla, Spain

    María-Cruz González, Rosario Sánchez & Francisco J. Cejudo

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  1. María-Cruz González
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  2. Rosario Sánchez
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  3. Francisco J. Cejudo
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Correspondence to Francisco J. Cejudo.

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González, MC., Sánchez, R. & Cejudo, F.J. Abiotic stresses affecting water balance induce phosphoenolpyruvate carboxylase expression in roots of wheat seedlings. Planta 216, 985–992 (2003). https://doi.org/10.1007/s00425-002-0951-x

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