Abstract
The aim of this work was to measure membrane currents activated by Ca release from the cardiac sarcoplasmic reticulum (s.r.). Intracellular Ca concentration ([Ca2+]i) was measured using fluo-3 in patch clamped cells. Calcium release from the s.r. (whether occurring spontaneously or evoked by caffeine) produced changes of membrane current which could be separated into a Ca-activated Cl current which was inhibited by DIDS or Cl removal and a Na-Ca exchange current. Both these currents had different time courses from the measured [Ca2+]i. Furthermore the Ca-activated Cl current decayed more quickly than did Na-Ca exchange. Possible explanations for the different kinetics of these two Ca-sensitive currents are discussed.
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Received: 21 November 1997 / Received after revision: 12 December 1997 / Accepted: 15 December 1997
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Trafford, A., Díaz, M. & Eisner, D. Ca-activated chloride current and Na-Ca exchange have different timecourses during sarcoplasmic reticulum Ca release in ferret ventricular myocytes. Pflügers Arch 435, 743–745 (1998). https://doi.org/10.1007/s004240050577
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DOI: https://doi.org/10.1007/s004240050577