Skip to main content
SpringerLink
Log in

Calcium transport pathways in the nucleus

  • Invited Review
  • Published:
Pflügers Archiv Aims and scope Submit manuscript

Abstract

Due to the availability of new biophysical and biochemical techniques, there has recently been considerable progress in our understanding of Ca2+ transport inside, as well as into and out of, the nucleus. A number of Ca2+ transport pathways have been localized specifically in the outer or inner nuclear membrane and the Ca2+ permeability through the nuclear pore complex has been assessed. The nuclear envelope has characteristics similar to those of a leaky epithelium. The leak is through the nuclear pore complex. The outer nuclear membrane contains the Ca2+ ATPase whereas the functionally important inositol trisphosphate (IP3)-activated Ca2+ release channels are specifically localized in the inner nuclear membrane.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Al-Mohanna FA, Caddy KWT, Bolsover SR (1994) The nucleus is insulated from large cytosolic calcium ion changes. Nature 367:745–750

    Article  PubMed  CAS  Google Scholar 

  2. Beaver JP, Waring P (1995) A decrease in intracellular glutathione concentration precedes the onset of apoptosis in murine thymocytes. Eur J Cell Biol 68:47–54

    PubMed  CAS  Google Scholar 

  3. Berridge MJ (1993) Inositol trisphosphate and calcium signalling. Nature 361:315–325

    Article  PubMed  CAS  Google Scholar 

  4. Bootman MD, Berridge MJ (1995) The elemental principles of calcium signalling. Cell 83:675–678

    Article  PubMed  CAS  Google Scholar 

  5. Brini M, Murgia M, Pasti L, Picard D, Pozzan T, Rizzuto R (1993) Nuclear Ca2+ concentration measured with specifically targeted recombinant aequorin. EMBO J 12:4813–4819

    PubMed  CAS  Google Scholar 

  6. Cullen PJ, Hsuan JJ, Truong O, Letcher AJ, Jackson TR, Dawson AP, Irvine RF (1995) Identification of a specific Ins (l,3,4,5)P4-binding protein as a member of the GAP1 family. Nature 376:527–530

    Article  PubMed  CAS  Google Scholar 

  7. Divecha N, Banfic H, Irvine RF (1991) The polyphosphoinosi- tide cycle exists in the nuclei of Swiss 3T3 cells under the control of a receptor (for IGF-1) in the plasma membrane, and stimulation of the cycle increases nuclear diacylglycerol and apparently induces translocation of protein kinase C to the nucleus. EMBO J 10:3207–3214

    PubMed  CAS  Google Scholar 

  8. Gerasimenko OV, Gerasimenko JV, Tepikin AV, Petersen OH (1995) ATP-dependent accumulation and inositol trisphosphate- or cyclic ADP-ribose-mediated release of Ca2+ from the nuclear envelope. Cell 80:439–444

    Article  PubMed  CAS  Google Scholar 

  9. Gerasimenko OV, Gerasimenko JV, Belan PV, Petersen OH (1996) Inositol trisphosphate and cyclic ADP ribose-mediated release of Ca2+ from single isolated pancreatic zymogen granules. Cell 84:473–480

    Article  PubMed  CAS  Google Scholar 

  10. Gillot I, Whitaker M (1993) Imaging calcium waves in eggs and embryos. J Exp Biol 184:213–219

    CAS  Google Scholar 

  11. Greber UF, Gerace L (1995) Depletion of calcium from the lumen of endoplasmic reticulum reversibly inhibits passive diffusion and signal-mediated transport into the nucleus. J Cell Biol 128:5–14

    Article  PubMed  CAS  Google Scholar 

  12. Hennager DJ, Welsh MJ, DeLisle S (1995) Changes in either cytosolic or nucleoplasmic inositol 1,4,5-trisphosphate levels can control nuclear Ca2+ concentration. J Biol Chem 270:4959–4962

    Article  PubMed  CAS  Google Scholar 

  13. Humbert J-P, Matter N, Artault J-C, Köppler P, Malviya AN (1996) Inositol 1,4,5-trisphosphate receptor is located to the inner nuclear membrane vindicating regulation of nuclear calcium signalling by inositol 1,4,5-trisphosphate. J Biol Chem 271:478–485

    Article  PubMed  CAS  Google Scholar 

  14. Kasai H, Li YX, Miyashita Y (1993) Subcellular distribution of Ca2+ release channels underlying Ca2+ waves and oscillations in exocrine pancreas. Cell 74:669–677

    Article  PubMed  CAS  Google Scholar 

  15. Lang I, Schulz M, Peters R (1986) Molecular mobility and nucleocytoplasmic flux in hepatoma cells. J Cell Biol 102:1183–1190

    Article  PubMed  CAS  Google Scholar 

  16. Lee HC (1994) A signalling pathway involving cyclic ADP-ribose, cGMP and nitric oxide. News Physiol Sci 9:134–137

    CAS  Google Scholar 

  17. Mak DO, Foskett JK (1994) Single-channel inositol 1,4,5-trisphosphate receptor currents revealed by patch clamp of isolated Xenopus oocyte nuclei. J Biol Chem 269:29375–29378

    PubMed  CAS  Google Scholar 

  18. Malviya AN, Rogue P, Vincendon G (1990) Stereospecific inositol 1,4,5-[32P] trisphosphate binding to isolated rat liver nuclei: evidence for inositol trisphosphate receptor-mediated calcium release from the nucleus. Proc Natl Acad Sci U S A 87:9270–9274

    Article  PubMed  CAS  Google Scholar 

  19. Maruyama Y, Petersen OH (1994) Delay in granular fusion evoked by repetitive cytosolic Ca2+ spikes in mouse pancreatic acinar cells. Cell Calcium 16:419–430

    Article  PubMed  CAS  Google Scholar 

  20. Maruyama Y, Shimada H, Taniguchi J (1995) Ca2+-activated K+ channels in the nuclear envelope isolated from single pancreatic acinar cells. Pflügers Arch 430:148–150

    Article  PubMed  CAS  Google Scholar 

  21. Matter N, Ritz M-F, Freyermuth S, Rogue P, Malviya AN (1993) Stimulation of nuclear protein kinase C leads to phosphorylation of nuclear inositol 1,4,5-trisphosphate receptor and accelerated calcium release by inositol 1,4,5-trisphosphate from isolated rat liver nuclei. J Biol Chem 268:732–736

    PubMed  CAS  Google Scholar 

  22. Means AR (1994) Calcium, calmodulin and cell cycle regulation. FEBS Lett 347:1–4

    Article  PubMed  CAS  Google Scholar 

  23. Newport J, Forbes DJ (1987) The nucleus: structure, function and dynamics. Annu Rev Biochem 56:535–565

    Article  PubMed  CAS  Google Scholar 

  24. Nicotera P, McConkey DJ, Jones DP, Orrenius S (1989) ATP stimulates Ca2+ uptake and increases the free Ca2+ concentration in isolated rat liver nuclei. Proc Natl Acad Sci U S A 86:453–457

    Article  PubMed  CAS  Google Scholar 

  25. Nicotera P, Orrenius S, Nilsson T, Berggren P-O (1990) An inositol 1,4,5-trisphosphate-sensitive Ca2+ pool in liver nuclei. Proc Natl Acad Sci U S A 87:6858–6862

    Article  PubMed  CAS  Google Scholar 

  26. Nicotera P, Bellomo G, Orrenius S (1994) Calcium-mediated mechanisms in chemically induced cell death. Annu Rev Pharmacol Toxicol 32:449–470

    Article  Google Scholar 

  27. Pante N, Aebi U (1994) Towards understanding the three-dimensional structure of the nuclear pore complex at the molecular level. Curr Opin Struct Biol 4:187–196

    Article  CAS  Google Scholar 

  28. Petersen OH (1992) Stimulus-secretion coupling: cytoplasmic calcium signals and the control of ion channels in exocrine acinar cells. J Physiol (Lond) 448:1–51

    CAS  Google Scholar 

  29. Petersen OH, Petersen CCH, Kasai H (1994) Calcium and hormone action. Annu Rev Physiol 56:297–319

    Article  PubMed  CAS  Google Scholar 

  30. Peunova N, Enikolopov G (1993) Amplification of calcium-induced gene transcription by nitric oxide in neuronal cells. Nature 364:450–453

    Article  PubMed  CAS  Google Scholar 

  31. Sullivan KMC, Busa WB, Wilson KL (1993) Calcium-mobilization is required for nuclear vesicle fusion in vitro: implications for membrane traffic and IP3 receptor function. Cell 73:1411–1422

    Article  PubMed  CAS  Google Scholar 

  32. Sweet DJ, Gerace L (1995) Taking from the cytoplasm and giving to the pore: soluble transport factors in nuclear protein import. Trends Cell Biol 5:444–447

    Article  PubMed  CAS  Google Scholar 

  33. Thorn P, Lawrie AM, Smith PM, Gallacher DV, Petersen OH (1993) Local and global cytosolic Ca2+ oscillations in exocrine cells evoked by agonists and inositol trisphosphate. Cell 74:661–668

    Article  PubMed  CAS  Google Scholar 

  34. Wegner M, Cao Z, Rosenfeld MG (1992) Calcium-regulated phosphorylation within the leucine zipper of C/EBP beta. Science 256:370–373

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. MRC Secretory Control Research Group, The Physiological Laboratory, University of Liverpool, Crown Street, P.O. Box 147, L69 3BX, Liverpool, UK

    O. V. Gerasimenko, J. V. Gerasimenko, A. V. Tepikin & O. H. Petersen

Authors
  1. O. V. Gerasimenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. V. Gerasimenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. V. Tepikin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. O. H. Petersen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gerasimenko, O.V., Gerasimenko, J.V., Tepikin, A.V. et al. Calcium transport pathways in the nucleus. Pflügers Arch — Eur J Physiol 432, 1–6 (1996). https://doi.org/10.1007/s004240050098

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s004240050098

Key words

Search

Navigation