Introduction

All terrestrial and some aquatic vertebrates have successfully evolved mechanisms to regulate ionic and osmotic homeostasis of their body fluids, which enables cellular activities and physiological processes to operate normally in these animals. In terrestrial vertebrates, these regulatory mechanisms are mainly performed by the kidneys. In aquatic vertebrates such as fish, the gills are the major extra-renal organ involved in body fluid ionic/osmotic homeostasis [28, 51, 52].

As a model organism, zebrafish (Danio rerio) has a number of advantages, including the availability of genetic databases, applicability of forward/reverse genetic manipulation and cell biological approaches, transparent embryos with rapid development, and short life cycle. As such, zebrafish is an emerging and competent model for developmental biology, neurobiology, and human diseases. Furthermore, several research groups have applied the zebrafish model to the study of integrative and regulatory physiology and, in particular, the epithelial transport that is associated with body fluid ionic/osmotic homeostasis. Zebrafish, a freshwater teleost fish, inhabits a hypotonic freshwater environment with low ionic concentrations (compared to those in the plasma) and fluctuant pH and therefore has to actively absorb ions from and secrete acid (or base) and ammonia to the environment, in order to maintain body fluid homeostasis. The majority of these tasks related to ion regulation are performed by the gills, and ionocytes within the gills are the major cell types involved in epithelial ion transport [28]. Recently, four types of zebrafish ionocyte were identified to be responsible for the transport of various ions by distinct sets of ion transporters (Fig. 1), and these ionocytes are analogous to the transporting cells in different segments of the mammalian kidney, in terms of the expression and function of ion transporters. During the embryonic stages before the gills are fully functional, ionocytes initially develop in the embryonic skin, becoming functional at around 24 h post-fertilization (hpf) (zebrafish embryos usually hatch between 48 and 72 hpf at 28.5 °C). Consequently, zebrafish embryos serve as a powerful in vivo model in which to study epithelial ion transport physiology and regulation, for the following reasons: First, ionocytes in embryonic skin are directly exposed to the environment, and their morphology and ion transporter expression can be readily observed in situ without further anatomic manipulation; second, ion fluxes (representing ion transporter function) can be directly examined by non-invasive electrophysiological approaches or the use of tracers (fluorescent probes or radioisotopes); third, the effects of transporter (or ionocyte) loss- or gain-of-function can be analyzed within 1–5 days post-fertilization, at which time the injected morpholinos or mRNAs are effective; and finally, incubation of zebrafish embryos (or adults) in media with different ionic compositions (including changes in Na+, Cl−, Ca2+, NH4 +, pH, or others) can facilitate investigation of transport function and regulation of a specific ion by the target transporter (or ionocyte).

Fig. 1
figure 1

Ionocytes in zebrafish. Four types of ionocyte have been identified: H+-ATPase-rich (HR), Na+-K+-ATPase-rich (NaR), Na+-Cl− cotransporter (NCC), and K+-secreting (KS) cells. Details of the transport pathways are given in the text (“Identification and functional analysis of ionocytes” section). AE1 anion exchanger 1b, CA2 carbonic anhydrase 2-like a, CA15) carbonic anhydrase 15a, ClC Cl− channel, ECaC epithelial Ca2+ channel, HA H+-ATPase, ROMK an ortholog of the mammalian renal outer medullary K+ channel (Kir1.1), NBC1 electrogenic Na+-HO3 − cotransporter 1b, NCX Na+/Ca2+ exchanger 1b, NHE Na+/H+ exchanger 3b, NAK.1∼5 Na+-K+-ATPase α1 subunit subtypes (atp1a1a.1∼5), PMCA plasma membrane Ca2+-ATPase 2, Rhcg1 rhesus glycoprotein. Question mark indicates unidentified transport pathways

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The topic of ion regulation in zebrafish has been summarized in some comprehensive fish reviews [23, 26, 27, 35, 50, 51, 68] and specific reviews on zebrafish [15, 49, 52]. The present review is intended to highlight the recent progress in the identification and functional analysis of ion transporters in different types of ionocyte, the ionocyte differentiation pathways, and functional regulation by hormones in zebrafish embryonic skin and gills.

Identification and functional analysis of ionocytes

H+-ATPase-rich ionocytes

H+-ATPase-rich (HR) ionocytes contain apical H+-ATPase (HA) and were first identified as the major cell type responsible for the acid secretion function of the embryonic skin and gills, as determined by a non-invasive scanning ion-selective electrode technique (SIET) and loss-of-function approach [42, 75]. Similar approaches were used to identify co-localization of Na+/H+ exchanger (NHE3b) and Rhesus glycoprotein (Rhcg1) at the apical membrane and Cl−/HCO3 − exchanger (AE1b) and Na+-K+-ATPase (NKA) α1 subunit (atp1a1a.5) at the basolateral membrane; they were also used to identify two carbonic anhydrases (membrane-bound CA15a and cytosolic CA2-like a) in HR ionocytes and to demonstrate that the epithelial transport functions of HR cells are related to not only H+ secretion but also to Na+ and HCO3 − uptake and ammonium excretion [24, 72, 73, 76, 83, 92, 119]. HR-type ionocytes in zebrafish are analogous to proximal tubular cells and type A intercalated cells of the kidney, in terms of transporter expression and function [13, 98, 113]. The activity of H+, NH4 +, and Na+, and Na+ accumulation can be examined in real time at a single ionocyte or in the body skin of a live embryo by SIET [75, 92, 93] and sodium green fluorescence microscopy [24], respectively. Given these advantages, HR cells in the zebrafish embryonic skin may provide an alternative in vivo model for research into the related epithelial transport physiology in vertebrates.

Both HA and NHE3b enable H+ secretion across the apical membrane of HR cells in zebrafish. Recent studies have shown that HA knockdown or bafilomycin A1 (an HA inhibitor) treatment suppressed 67–75 % of the apical H+ activity in HR cells [91], while NHE3b knockdown or EIPA (an NHE3 inhibitor) treatment only impaired 27–29 % of the activity [93]. Therefore, it seems that HA plays a greater role than HNE3b in the acid secretion function of zebrafish HR cells. HA-linked epithelial Na+ channel (ENaC) and NHE have been long considered the two major Na+ uptake pathways in fish [28, 51]. Esaki et al. [24] provided pharmacological evidence to support the role of HA, ENaC, and NHE3b in apical Na+ uptake in zebrafish: bafilomycin A1, amiloride (10−5 and 10−4 M), and EIPA suppressed Na+ influx (as detected by 24 Na+ tracing) or Na+ accumulation (as detected by sodium green (10−5 M amiloride had no effect)) in embryonic skin HR cells. Notably, orthologs of mammalian ENaC have not been detected in the genomes of zebrafish and other teleosts, and thus, the identity of the ENaC in fish remains to be determined. Supporting Esaki’s data, loss of function of HA was found to decrease Na+ content in zebrafish embryos reared in a low-Na+ medium [42]. On the other hand, a recent study by Kumai and Perry [67] did not find a significant effect of EIPA on Na+ influx in zebrafish embryos reared in normal freshwater (the similar ion compositions in freshwater and inhibitor concentration as in Esaki’s study), bringing the role of NHE3b in zebrafish Na+ uptake into question. Further investigation is required to reconcile the inconsistencies between the two studies. To this end, we found that NHE3b knockdown reduced zebrafish Na+ content under either normal freshwater (unpublished data by Chang WJ and Hwang PP; Fig. 2) or low-Na+ freshwater [93] conditions, and this more convincing molecular physiological approach supports a role for NHE3b in the Na+ absorption function of HR cells.

Fig. 2
figure 2

Effect of nhe3b knockdown on Na+ content in zebrafish embryos at 3 day post-fertilization. Specific morpholinos (following [92]) were microinjected into 1∼4 cell-stage embryos incubated in normal freshwater (about 500 μM NaCl and 20 μM Ca2+). Whole-body Na+ content was measured by atomic absorption spectrophotometry. Data are expressed as the mean ± SD (n = 6). A significant difference (Student’s t test, p < 0.001) was found between wild-type (WT) and nhe3b morphant embryos

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Unlike mammals, which excrete urea as the major carrier of waste nitrogen, teleosts are predominantly ammonotelic, i.e., they mainly excrete ammonia. As teleosts can directly excrete ammonia into the surrounding water, they do not need to waste energy in converting it into less-toxic urea. Most ammonia excretion is accomplished by the gills or skin (the latter during embryonic stages). Our understanding of the mechanism and regulation of ammonia excretion and its associated roles in fish gill/skin Na+ uptake and acid secretion by specific types of ionocyte has been increased by the recent discovery of Rh proteins and the application of the SIET technique to fish embryos. Fish gills were thought to predominately excrete NH3 gas rather than ionic NH4 +, based on early studies on “acid-trapping” ammonia excretion, through which acidification of the boundary layer adjacent to the gill surface facilitates the excretion of ammonia [116]. In studies on rainbow trout, CO2 excretion by the gills was suggested to be the major source of the acidic layer, and Rhcg, HA, NHE, and ENaC were proposed to work together as a functional metabolon [117]. However, prior to recent studies on zebrafish, there was no evidence to support co-localization of these transporters, or in vivo molecular physiological data to support the proposed model. Rhcg1 has since been shown to co-localize with HA and NHE3b in the apical membrane of zebrafish HR cells [83, 92], and knockdown of Rhcg1 was found to suppress apical NH4 + excretion in the cells [92]. Moreover, both HA (or NHE3b) knockdown or treatment with bafilomycin A1 (or EIPA) resulted in a decrease in apical H+ activity accompanied by impaired NH4 + excretion, providing in vivo evidences to demonstrate that HA or NHE3b may provide an alternative H+ source for “acid-trapping” ammonia excretion in zebrafish [92, 93]. Besides Rhcg1, Rhag and Rhbg were also found to be involved in zebrafish NH4 + excretion, as determined from loss-of-function experiments; however, the pattern of mRNA and protein expression for these two transporters does not appear to be related to specific ionocytes [11].

The coupling of NHE3 and Rhcg1 not only mediates acid-trapping ammonia excretion but also mediates Na+ uptake by ionocytes. By using SIET to probe the real-time Na+, NH4 +, and H+ gradients of ionocytes in the embryonic skin of medaka, another teleost species, Wu and colleagues [118] found that Na+ uptake and ammonia excretion by ionocytes are tightly associated. Acute exposure to high NH4 + was found to simultaneously suppress NH4 + excretion and Na+ uptake in zebrafish embryos acclimated to low-Na+ and low-pH water [67, 93]. Moreover, acclimation to low-Na+ media stimulated transcription of the genes encoding Rhcg1 and NHE3b in the gill, as well as NH4 + excretion/Na+ uptake in zebrafish [93]. Moreover, X-ray crystallographic studies on the molecular structures of bacterial homologues of Rh proteins, AmtB and NeRh, suggested these proteins form ammonia channels that recruit intracellular NH4 +, release H+, and transport NH3 through the transmembrane hydrophobic pore, ultimately releasing NH4 + by recruiting H+ to the extracellular side [55, 61]. This deprotonating and protonating action on the inside and outside of the membrane, respectively, may create a favorable H+ gradient to drive the operation of electroneutral NHE3b in zebrafish HR cells. This model was recently supported by genetic knockdown experiments, in which loss of function of Rhcg1 decreased both NH4 + excretion and Na+ uptake in zebrafish embryos acclimated to low-Na+ water [93] or acidic water [67]. Unlike mammalian renal cells, gill/skin ionocytes in aquatic animals encounter freshwater with less than 1 mM Na+, a theoretically thermodynamic constraint on the operation of NHE [4]. The proposed ammonium-dependent sodium uptake model [52, 67, 93, 118] may help resolve the long-debated questions regarding the driving mechanisms of NHE in the ionocytes of freshwater fish or other aquatic animals.

Epithelial Na+ uptake and H+ secretion (equivalent to HCO3 − uptake) in zebrafish HR cells requires carbonate anhydrases and other basolateral transporters, a requirement shared by mammalian kidney proximal tubules and collecting ducts [87]. In zebrafish HR cells, apical NHE3b and H+-ATPase transport H+ out of cells, and H+ combines with environmental HCO3 − to generate H2O and CO2, as catalyzed by CA15a (an ortholog of mammalian CA4). CO2 enters cells and is hydrated by Ca2 like-a (an ortholog of mammalian CA2) to form H+ and HCO3 −. Basolateral AE1b (an ortholog of mammalian AE1) extrudes cytosolic HCO3 − down the Cl− gradient to fulfill the epithelial acid-secreting function, while basolateral NKA is responsible for the excretion of Na+. On the other hand, K+-Cl− cotransporter (KCC) recycles the Cl− accumulated by the action of basolateral AE1 in mammalian type A intercalated cells [107]. Further studies are needed to determine if an ortholog(s) of KCC is also expressed and involved in Cl− recycling in zebrafish HR cells.

Na+-K+-ATPase-rich ionocytes

Na+-K+-ATPase-rich (NaR) cells were initially identified as a specific group of ionocytes distinct from HR cells through labeling of zebrafish embryonic skin with an anti-chicken NKA α subunit monoclonal antibody (α5, Developmental Studies Hybridoma Bank, University of Iowa) [74] (Fig. 1). A minority (about 20–30 %) of NaR cells express epithelial Ca+ channel (ECaC) mRNA during early embryonic stages (around 36 hpf), and the number of NaR cells expressing ECaC increases following embryonic development, reaching up to 90 % in adult gills [74, 85]. Six isoforms of plasma membrane Ca2+-ATPase (PMCA) and seven isoforms of Na+/Ca2+ exchanger (NCX) have been identified in zebrafish, of which only PMCA2 and NCX1b were found to co-localize in NaR cells, as determined by triple in situ hybridization and immunocytochemistry [74]; zebrafish NaR cells exhibit a similar set of Ca2+ transporters to mammalian renal and intestinal Ca2+-absorption cells [40]. Moreover, of six NKA α1 subunit genes, only atp1a1a.1 was expressed in NaR cells [73]. The physiological role of ECaC in Ca2+ uptake in zebrafish was demonstrated by knockdown with specific morpholinos [104] and by low-Ca2+ acclimation, which was found to stimulate ECaC mRNA expression and Ca2+ influx and increase the number of ECaC-expressing ionocytes [74, 85]. In mammals, transepithelial Ca2+ transport through apical influx, cytosolic diffusion, and basolateral efflux are tightly connected by TRPV5/6 (orthologs of zebrafish ECaC), NCX1, and PMCA1b; however, TRPV5 (or TRPV6) transporters appear to constitute the rate-limiting step for Ca2+ uptake [40]. A similar model was proposed early on for fish gills [30], but molecular evidence for the identity of the relevant transporters was lacking until recent studies on zebrafish ([74, 85]; see above). An early enzyme kinetic study found that the basolateral Ca2+ extrusion mediated by PMCA and NCX in fish gills is far below its maximum capacity [29]. Acclimation of zebrafish to a low-Ca2+ environment, which is known to stimulate Ca2+ uptake [85], was also found to increase mRNA expression of ECaC, but not zPMCA2 or zNCX1b [74]. This implies that steady-state expression of PMCA2 and NCX1b may support transepithelial transport under most environmental conditions. Moreover, expression of ECaC, but not that of PMCA2 and NCX1b, is affected by hormones, including stanniocalcin [104], cortisol [78], and vitamin D [77]. Taken together, these findings suggest that the role of ECaC as the major regulatory target for transepithelial Ca2+ uptake is conserved from fish to mammals.

The expression patterns of Ca2+ transporter isoforms differ between mammals and zebrafish, and this may be advantageous for elucidating mammalian Ca2+ handling in a zebrafish model. In zebrafish, the NCX1b isoform is required for Ca2+ extrusion in gill/skin ionocytes, and the NCX1a isoform is expressed specifically in heart; mutations in the latter cause cardiac fibrillations [71]. Conversely, a single NXC1 performs the roles of both NCX1a and NCX1b in mammals, thus making it difficult to investigate the specific role of NCX1 in renal Ca2+ transport by gene knockout without inducing cardiac defects and mortality [40, 65]. As such, zebrafish may provide an alternative model in which to study the role and functional regulation of NCX1a in body fluid Ca2+ homeostasis without inducing heart failure. Another difference in isoform number between species is observed for ECaC: A single isoform is required for transepithelial Ca2+ transport in zebrafish, while two mammalian isoforms, TRPV5 and TRPV6, function as the apical Ca2+ entry pathway of the transepithelial Ca2+ transport mechanisms in mammalian kidneys and intestines. Studies on this pathway and its functional regulation by single knockdown of TRPV5 (or TRPV6) are complicated by functional redundancy and compensation between the two paralogous genes [40, 105]. A zebrafish mutant with a loss of function of ECaC (matt-und-schlapp) was found to exhibit impaired Ca2+ uptake and severe defects in bone formation, suggesting this mutant may serve as an alternative in vivo model for the study of this essential physiological process [105].

Na+-Cl− cotransporter-expressing ionocytes

Another subtype of zebrafish skin/gill ionocytes, Na+-Cl− cotransporter (NCC) cells, was recently found to be analogous to the mammalian distal convoluted tubular cells that express Na+-Cl−-cotransporters and participate in the absorption of Na+ and Cl− [111] (Fig. 1). In zebrafish, slc12a10.2 (an NCC-like member distinct from slc12a3 (an ortholog of mammalian SLC12A3)) is localized to a specific group of ionocytes that are different from the previously identified HR and NaR cells. Furthermore, metolazone (a specific NCC inhibitor) has dose-dependent inhibitory effects on influx of both Na+ and Cl− in intact embryos [111]. NCC loss-of-function decreased the Cl− content in the morphants, demonstrating the physiological role of NCCs in transepithelial Cl− uptake in zebrafish [111]. As Na+ uptake in zebrafish occurs mainly through the actions of NHE3b ([25, 119]; see “H+-ATPase-rich ionocytes” section), NCC may play a minor or supplemental role. Metolazone was reported to have no effect on sodium green (a fluorescent Na+ probe) accumulation in zebrafish HR cells [24], and NCC knockdown resulted in an increase in Na+ content and a concomitant stimulation of NHE3b expression in 5-day pf morphants [111]. In addition, knockdown of glial cell missing 2 (encoding Gcm2, a transcription factor that specifically affects differentiation of NHE3b-expressing HR cells; see “Proliferation and differentiation of ionocytes” section) resulted in a loss of HR cells (thus impairing Na+ uptake) with a concomitant increase in the number of NCC cells in zebrafish morphants [51, 97]. Interestingly, this functional redundancy between NHE3 and NCC in Na+ homeostasis appears to be conserved in zebrafish and mammals. In mammalian kidneys, proximal tubules perform bulk reabsorption of Na+ via NHE3, and further, fine-tuning of Na reabsorption is achieved by other redundant pathways, including the distal convoluted tubular NCC [36].

The lumen of mammalian distal convoluted tubules contains a Na+ concentration of 35–77 mM to drive the operation of electroneutral NCC [37]. Unlike the mammalian NCC, the zebrafish NCC may not, theoretically, be driven by the 0.4–0.7-mM Na+/Cl− concentration of ambient freshwater (local tap water) to which they are exposed. According to recent SIET measurements at the apical membrane of tilapia embryonic ionocytes [41], the activity of Na+/Cl− in the microenvironment appeared to be higher than that in ambient freshwater, probably due to outward Na+/Cl− fluxes from neighboring pavement cells, and these ionic gradients may be high enough to drive the NCC. However, this hypothesis remains to be tested in zebrafish NCC cells. It may be addressed by examining whether or not the zebrafish NCC is different from its mammalian equivalent in terms of transport kinetics or stoichiometry.

Basolateral transporters in zebrafish NCC ionocytes include NKA α1 subunit (atp1a1a.2) and Na+-HCO3 − cotransporter (NBCe1b) [72, 73]. The subunit atp1a1a.2 was found to be specifically localized in NCC ionocytes, and its mRNA expression is stimulated by low-Cl− freshwater, suggesting its involvement in the Cl− uptake mechanism of NCC cells [73, 111]. Of the 12 isoforms of the zebrafish slc4a family, only two, slc4a1b (AE1b) and slc4a4b (NBCe1b), exhibited a salt-and-pepper-like ionocyte pattern in mRNA expression in zebrafish skin and gill [72, 95, 96, 100]. AE1b is co-expressed with NHE3b/HA in HR ionocytes as described above, while NBCe1b and NCC are co-expressed in NCC cells [72]. Apical NCC transports Na+ into the cells, and basolateral NBCe1b and NKA (atp1a1a.2) may extrude intracellular Na+ out of the cells; however, molecular physiological evidence in support of these functions is not yet available. In mammalian distal convoluted tubular cells, the Cl− channels, ClC-K1 and ClC-K2, act as basolateral transporters to extrude Cl− [88]. An ortholog of mammalian ClC co-localizes with NCC in gill ionocytes of tilapia, a teleost species [102]. Future identification of ClC member(s) in zebrafish is necessary for a comprehensive understanding of the transepithelial transport mechanisms in zebrafish NCC ionocytes.

K+-secreting ionocytes

The mRNA of a subtype of the NKA α1 subunit (atp1a1a.4) was found to be expressed in a specific group of ionocytes in zebrafish, distinct from the HR, NaR, and NCC ionocytes [73] (Fig. 1). Identification and functional analysis of these atp1a1a.4-expressing cells remained unresolved [51, 52, 73] until a recent study investigating a K+ channel, Kir1.1, an ortholog of the mammalian renal outer medullary K+ channel (ROMK) [1]. Kir1.1 mRNA was reported to be co-localized with atp1a1a.4 in the same type of ionocytes in zebrafish embryonic skin, and loss of function of Kir1.1 resulted in transient tachycardia followed by bradycardia [1], effects similar to that observed in hyperkalemia in human [59]. These Kir1.1-expressing ionocytes were proposed to be K+-secreting (KS) cells [1], although there is no physiological evidence to demonstrate K+ currents in KS cells. In mammalian kidneys, the ROMK protein is localized to the apical membrane of cells of the thick ascending limb and distal nephron and is required for the K+ secretion pathway and thus K+ homeostasis, as well as being involved in driving the relevant transporters to absorb Na+ and/or Cl− and paracellular Na+/Ca2+/Mg2+ transport [39, 114]. From an evolutionary point of view, the physiological functions of the mammalian ROMK may also be conserved, to a certain extent, in zebrafish. Identification of other co-expressed transporters and functional analysis of those transporters in the presumed KS cells are necessary to explore the physiological roles of KS cells in zebrafish.

Other transporting ionocytes

In the mammalian collecting duct, type B intercalated cells co-express apical pendrin (SLC26A4) and basolateral HA and play a major role in HCO3 − secretion (equivalent to H+ absorption) for body fluid acid–base homeostasis [107, 109]. Similarly, three orthologs of mammalian pendrin, slc26a3, slc4, and slc6c, were recently identified in zebrafish [8, 86]. The encoding mRNA of these proteins (and slc26a3 protein itself) is expressed in certain type(s) of cells in the embryonic and adult gills, and a minority (<10 %) of slc26a3-expressing cells also co-expresses basolateral NKA [8, 86]. Acclimation to low-Cl− or high-NaHCO3 freshwater was found to stimulate the transcription of these three genes in the gill and to enhance Cl− uptake in adult zebrafish, suggesting a role for these genes in apical Cl−/HCO3 − exchange in zebrafish cells [86]. This hypothesis was further supported by loss-of-function experiments in which knockdown of all three genes impaired Cl− uptake in morphants raised in low-Cl− freshwater [8]. Basolateral HA and/or other enzymes (e.g., carbonic anhydrases) are essential for transepithelial Cl− uptake/HCO3 − secretion. Whether these proteins co-localize with slc26 proteins, and if so, the functional implications of this co-localization, remain to be addressed. Moreover, the identity of the driving mechanism for apical Cl−/HCO3 − exchange in zebrafish gill/skin ionocytes encountering a freshwater environment remains unclear, but investigation of the stoichiometry of the zebrafish slc26 members may provide answers. It is unknown if these slc26 members are expressed in the same type of cells or in one (or more) type of the identified ionocytes (HR, NaR, NCC, or KS cells), and therefore, the slc26-expressing cells are not presented in the proposed model (Fig. 1).

Proliferation and differentiation of ionocytes

Proliferation and differentiation of ionocytes are means by which ionocyte function is regulated in zebrafish during acclimation to harsh environments. These processes have become more amenable to study following the recent use of genetic and molecular approaches to explore the molecular mechanisms of ionocyte development [52]. Ionocytes are derived from non-neural ectoderm that express ΔNp63, a marker (and requirement) of proliferating epithelial cells [54]. After gastrulation (bud stage), two specification markers of ionocytes, forkhead box I3a (Foxi3a) and DeltaC (a ligand of Notch signaling), are expressed in certain ΔNp63-positive epidermal cells that further differentiate into ionocyte progenitors [45]. The epidermal ionocyte progenitors undergo lateral inhibition through Notch signaling to inhibit the expression of Foxi3a in neighboring cells; Notch signaling maintains the cell fate of the keratinocyte lineage [45]. As development proceeds, ΔNp63 expression in epidermal ionocyte progenitors is downregulated at the 14-somite stage, and Foxi3a activates cell differentiation of ionocytes [45].

At least four subtypes of ionocytes have been identified: HR, NaR, NCC, and KS cells (see “Identification and functional analysis of ionocytes” section). However, the mechanisms underlying ionocyte subtype specification remain unclear. Hsiao et al. [45] demonstrated that Foxi3a turns on the expression of Foxi3b at the 5-somite stage and that the positive regulatory loop between Foxi3a and Foxi3b functions as a master regulator for specifying epidermal ionocyte progenitors [45]. Later on, Foxi3a is downregulated in NaR cells, while Foxi3b is downregulated in HR cells [45, 54]. Foxi3a and Foxi3b expression in HR cells and NaR cells seems to be critical for the specification of ionocyte subtypes [24, 45, 54]. Foxi3a also induces expression of Jagged 2a and Jagged 2b, which are Notch ligands expressed in differentiating ionocytes; this suggests that Notch signaling may also be involved in the specification of ionocyte subtypes [54].

In addition to Foxi3 and Notch signaling, a cell fate-related transcription factor, Gcm2, has been reported to control the specification of HR cells [14]. Knockdown of Gcm2 completely abolished HR cell formation, but had no effects on NaR cell density at 72 hpf, indicating that Gcm2 is essential for differentiation of HR, but not NaR cells [14]. Gcm2 may control HR cell differentiation via interactions with Foxi3a. Esaki et al. [25] demonstrated that knockdown of Gcm2 suppressed Foxi3a expression at 24 hpf [25]. This suggests that Gcm2 may modulate expression of Foxi3a and Foxi3b and, as a result, determine the NaR and HR cell lineages. Esaki et al. [25] also identified another transcription factor, Foxi1, which is required for HR cell differentiation [25]. Knockdown of Foxi1 decreased expression of DeltaC and Foxi3a and impaired formation of HR cells [25]. However, NaR cell differentiation was unaffected in Foxi1 morphants, making the role of Foxi1 in ionocyte differentiation unclear. Alternatively, Foxi1 may control HR cell differentiation through an indirect pathway.

Ionocyte differentiation in adult zebrafish remains poorly understood; however, recent studies have indicated that differentiation of adult ionocytes is also mediated through Foxi3a [18, 21]. During cold acclimation, zebrafish ionocyte differentiation is stimulated through an increase in Foxi3a expression, and both cell proliferation and apoptosis in the gill epithelial cells are decreased to extend the cell lifespan for enhancing ionocyte function [18]. An in vitro experiment using cultured adult zebrafish gills revealed that cortisol treatment upregulated expression of Foxi3a and Foxi3b and increased ionocyte numbers in the gills [21]. Moreover, after acclimatizing to an acidic environment, Gcm2 expression and HR cell numbers were increased in the gills of adult zebrafish, suggesting that the H+ secreting function of HR cells in the gills is enhanced by a Gcm2-mediated acceleration of HR cell differentiation [14]. It seems likely that differentiation of adult gill ionocytes shares the same or similar regulatory pathways with that of embryonic ionocytes.

Zebrafish ionocytes are analogous to mammalian kidney cells in terms of the expression and function of the relevant transporters, as described above. Interestingly, zebrafish ionocytes exhibit a conserved cell differentiation pathway, as also observed for mammalian intercalated cells [2]. Previous studies have indicated that type B intercalated cells are the progenitors of the type A cells, and this conversion arises from hensin (an extracellular matrix protein)-mediated differentiation plasticity in response to changes in extracellular pH [33, 90]. Intercalated cells are derived from the ureteric bud, an epithelial structure that buds off the Wolffian duct [58, 99]; Notch signaling determines the cell lineages of principle and intercalated cells [56]. In addition, Foxi1 was reported to be critical for the differentiation of intercalated cells, based on the finding that intercalated cells were absent from the collecting duct in Foxi1-null mice [9, 106]. A retrospective view of the related research indicates that the discovery of the role of Foxi1 in intercalated cell differentiation [9] gave rise to pioneer studies on zebrafish Foxi3a/3b [24, 45, 54] and subsequently the first model for the molecular pathway of zebrafish ionocyte development [45, 50]). It is interesting to compare the current zebrafish model (Fig. 3) with the recently proposed model for mammalian intercalated cell differentiation [2]. Both development of zebrafish ionocytes and mammalian intercalated cells appear to involve similar signaling (i.e., the forkhead transcription factors and Notch), and the zebrafish model may provide leads to enhance our understanding of cell specification and differentiation in the mammalian kidney. Zebrafish ionocytes, which are located in external organs, skin, or gills, are easily traced during differentiation, and the function of each cell subtype can be readily investigated. As mentioned above, zebrafish possess several advantages, including ease of gene manipulation and transgenic line generation. Taken together, zebrafish ionocytes may be a highly suitable in vivo model or research platform for studies into the physiology and development of transporting cells in mammals and other animals and may bring new insights to related fields.

Fig. 3
figure 3

Model of the differentiation pathways of zebrafish skin ionocytes. A detailed explanation of the pathways is given in the text (“Proliferation and differentiation of ionocytes” section). NaR Na+-K+-ATPase-rich, HR H+-ATPase-rich

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Functional regulation of ionocytes

Zebrafish directly encounter hypotonic freshwater, which is dynamic in its ionic concentration and composition. In laboratory acclimation experiments, zebrafish survive and behave normally in extremely soft ([Na+] = 0.03–0.1 mM, [Cl−] = 0.03–0.04 mM, [Ca2+] = 0.02–0.05 mM) [10, 17, 20, 111, 119], acidic (pH 4) or alkaline (pH 10) waters [43], and water containing high ammonia (5 mM NH4 +) [92]. Environmental changes directly disturb zebrafish body fluid ionic/osmotic homeostasis, and zebrafish have to quickly and effectively regulate their transepithelial ion transport functions to make compensatory adjustments. These regulatory processes include (1) a prompt adjustment, on the scale of minutes to hours, of pre-existing transporters or ionocytes and (2) long-term regulation, on the scale of hours to days, of transporter expression and ionocyte number and thus overall functional capacity. In a live medaka embryo, sequential and real-time probing of the same ionocytes in the skin revealed that a functional change from inward Na+/Cl− currents (NaCl uptake) to outward currents (NaCl secretion) was achieved within 2∼5 h of the transfer from freshwater to seawater [91]. Similarly, the apical surface structure (labeled with ConA, a membrane marker) and H+ activity (measured by SIET) within the HR ionocytes of zebrafish embryos were modulated within 10∼120 min of acute transfer from pH 7 to pH 4 [52]. These rapid regulatory responses may be mediated by the trafficking of HA molecules in HR cells, as for intercalated cells of the mammalian kidney [12]. A recent study on zebrafish micro(mi)RNA 8 [31] shed further light on this issue. Nherf1, a regulator of apical trafficking of ion transporters, was found to be associated with Na+ uptake in zebrafish HR cells, and miRNA 8 was demonstrated to regulate Nherf1 expression and apical membrane trafficking/clustering (monitored by ConA staining) and thus Na+ uptake (sodium green accumulation) [31].

Acclimation to ion (Ca2+, Na+, or Cl−) deficient, high NH4 +, or acidic freshwater for several days to 2 weeks has been reported to result in increased expression of genes encoding related ion transporters [8, 20, 43, 76, 85, 92, 119] and increased numbers of ionocytes in zebrafish embryos or adult gills [14, 43, 85]. A recent study further explored the cellular events associated with these regulatory processes [43]. A 4-day acclimation to pH 4 freshwater induced a compensatory enhancement in acid secretion by zebrafish embryos, and this functional enhancement of HR cells was achieved not only by increasing the cell number but also by increasing the acid-secreting function (as measured by SIET) of single cells [43]. Further labeling experiments demonstrated that additional HR cells in pH 4 water originated from differentiation of both ionocyte precursor cells and newly proliferating epithelial stem cells [43], and loss-of-function experiments revealed that such differentiation was stimulated by Gcm2 [14].

Hormonal control of ion regulation

Hormones are known to tightly control the ionic homeostasis of body fluid, and many of the functions and actions of hormones on ion regulation are common to all vertebrates [80]. Here, we summarize recent findings on the hormonal control of ion regulation in zebrafish.

Regulation of Ca2+ uptake

Different hormones participate in the control and regulation of Ca2+ homeostasis [44]. To date, seven hormones have been proposed to be involved in Ca2+ regulation in zebrafish, namely prolactin (PRL), growth hormone (GH), parathyroid hormone (PTH1), cortisol, vitamin D, calcitonin (CT), and stanniocalcin (STC-1) [44, 47, 70, 77, 78, 104]. These hormones exhibit both positive and negative regulatory actions through complicated network acting on body fluid Ca2+ homeostasis in zebrafish. In zebrafish embryos, transcription of the genes encoding PRL, GH, and PTH1 was stimulated upon acclimation to 1/20× diluted freshwater (34∼40 μM NaCl, 0.8 μM Ca2+), and transcription returned to the original levels observed in normal freshwater (680∼800 μM NaCl, 16 μM Ca2+) if the 1/20× freshwater was supplemented with 16 μM CaCl2; this suggests that the affected hormones have roles in Ca2+ homeostasis and bone formation [44]. PRL and GH are well-known as freshwater-acclimation- and seawater-acclimation hormones, respectively [28, 80]. However, the exact role of GH in body fluid ionic homeostasis in zebrafish, a stenohaline freshwater species, is unknown. Similar to its mammalian counterpart, zebrafish PTH appears to be a regulator of Ca2+ homeostasis, although teleosts lack anatomically distinct parathyroid glands [115]. A role for zebrafish PTH in Ca2+ homeostasis has been supported by several recent studies (see below).

The recently established model for the roles of zebrafish Ca2+-transporting ionocytes (see “Na+-K+-ATPase-rich ionocytes” section) provides a competent platform to precisely study the target ion transporters of Ca2+ regulatory hormones. Several hormones are proposed to regulate ECaC expression to control body fluid Ca2+ homeostasis. Acclimation to low-Ca2+ freshwater was reported to stimulate Ca2+ influx and expression of ECaC, 11β-hydroxylase, and the glucocorticoid receptor (GR) [78]. Exogenous cortisol was found to increase Ca2+ influx and expression of ECaC and hydroxysteroid 11-beta dehydrogenase 2; expression of 11b-hydroxylase and the GR, however, was decreased, and other Ca2+ transporters (PMCA and NCX) and the mineralocorticoid receptor (MR) were unaffected [78]. These findings suggest an involvement of cortisol-GR signaling in the stimulation of Ca2+ uptake. This hypothesis was further supported by loss-of-function experiments; morpholino knockdown of the GR, but not the MR, impaired zebrafish Ca2+ uptake function by inhibiting ECaC expression [78]. In mammals, both glucocorticoids and mineralocorticoids are involved in regulating body fluid osmolality and ion levels [16, 81]. Glucocorticoids decrease body fluid Ca2+ levels through suppressing the TRPV6 and calbindin-D9K in the kidney and duodenum [48, 64]. Due to a lack of aldosterone synthase, cortisol is the main corticosteroid in fish [6, 84]. Cortisol can bind to both the GR and MR, albeit with different affinities, and each signaling pathway has different effects on the transcription of genes encoding ion transporters in the gills [63]. The different effects of cortisol on Ca2+ regulation in mammals and fish may reflect the differences in the Ca2+ absorption pathways between terrestrial and aquatic vertebrates. On the other hand, a recent study used a heterologous antibody to reveal that GR is not localized to zebrafish NaR cells (the ionocytes responsible for Ca2+ absorption, see “Na+-K+-ATPase-rich ionocytes” section), questioning about the role of cortisol in zebrafish Ca2+ uptake [66]. The in situ hybridization with a specific GR RNA probe, however, demonstrated the presence of GR mRNA in NaR and other types of cell in the zebrafish gill [78]. Subtle differences between the methodologies or antibody specificity may be responsible for this inconsistency, but this awaits confirmation.

Bioactive vitamin D is well-documented as a vital hormone regulating Ca2+ uptake in mammals. Complexes of vitamin D3 and the vitamin D receptor (VDR) can upregulate mammalian ECaC translation by binding to the VDR responsive element (VDRE) of ECaC and thereby enhance Ca2+ absorption [40]; this is consequently an important pathway for the control of Ca2+ homeostasis in mammals. The zebrafish ECaC also possesses putative VDREs; however, the target cells or transporters of vitamin D-VDR signaling in fish teleosts were unknown until recently [77]. As for exogenous cortisol, exogenous vitamin D was also reported to increase Ca2+ influx and ECaC expression, with no effects on PMCA and NCX; furthermore, exogenous vitamin D increased 25-hydroxyvitamin D3-24-hydroxylase expression but decreased 1α-hydroxylase (cyp27b1) expression. Vitamin D-mediated enhancement of ECaC expression/function was found to occur through interaction with only one of the duplicate VDRs in zebrafish (VDRa), based on loss-of-function analyses of the two receptors [77]. In addition, exogenous cortisol treatment stimulated, while knockdown of the GR (but not the MR) suppressed transcription of the genes encoding VDRa, cyp27a1 and cyp27b1, indicating that cortisol-mediated increases in ECaC expression enhance Ca2+ uptake via GR and vitamin D signaling [77, 78].

In addition to calciotropic hormones, hypocalcemic hormones are also involved in the regulation of Ca2+ uptake. STC-1 is secreted from the corpuscles of Stannius that are attached to the kidney. Surgical removal of the corpuscles of Stannius causes hypercalcemia, suggesting a hypocalcemic function of STC-1. In zebrafish embryos, high-/low-Ca2+ acclimation experiments and loss-/gain-of-function experiments revealed that STC-1 reduces Ca2+ uptake in response to excess Ca2+ in the body by negatively regulating ECaC expression [104]. Human STC-1 also exhibits anti-hypercalcemic actions [108]. Mammalian STC-1 decreases Ca2+ uptake across the intestines and kidney [110, 121], which presumably also occurs through decreased ECaC expression. The signal pathways underlying STC-1-mediated inhibition of ECaC expression in both mammals and fish remain unknown, due to a lack of information on the STC-1 receptor. Discovery and characterization of the STC-1 receptor are required to further elucidate the roles of STC-1. As regards the regulation of STC-1, a recent study utilized a forward genetic mutagenesis screen and positional cloning to identify macrophage-stimulating protein (Msp), as a novel Ca2+ homeostasis modulator that may act upstream of zebrafish STC-1 [47]. In situ hybridization of the receptor for Msp, Ron, revealed the specific expression in the corpuscles of Stannius; Ron knockdown morphants and msp mutant embryos presented with identical phenotypes, which could be rescued by excess Ca2+ [47]. The msp mutant embryos had higher levels of STC-1, ECaC, PTH1, and PTH2 expression and elevated body Ca2+ content as compared to wild-type embryos [47]. These results suggest that the Msp/Ron signaling pathway controls Ca2+ homeostasis, most likely through interactions with other hormones, such as STC-1 and PTH1.

CT is another hypocalcemic hormone in zebrafish and mammals. Zebrafish embryos treated with high-Ca2+ water was found to upregulate expression of CT and its receptor (CTR), but downregulate expression of ECaC [70]. Knockdown of CT stimulated ECaC expression and suppressed CTR, while overexpression of CT inhibited ECaC expression in zebrafish embryos incubated in high-Ca2+ freshwater, supporting an anti-hypercalcemic role for CT [70]. In mammals, CT appears to inhibit intestinal Ca2+ absorption and renal Ca2+ reabsorption [62], but this function remains controversial. Microperfusion experiments in rabbits demonstrated that CT stimulates Ca2+ absorption in distal convoluted tubules where TRPV5 is not expressed [94]. Injection of CT into mice also stimulated renal Ca2+ reabsorption without affecting TRPV5 expression, and similar effects were observed in TRPV5−/− mice, suggesting that the CT acts independently of TRPV in renal Ca2+ reabsorption [46].

ECaC appears to be the primary target in hormone-mediated regulation of epithelial Ca2+ transport, as the other Ca2+ transporters, PMCA and NCX, are apparently unaffected. However, basolateral PMCA and NCX may still be targeted by regulatory mechanisms under some conditions. For example, overexpression of CT suppressed ECaC and stimulated STC-1 expression in zebrafish embryos at 30 hpf but upregulated expression of several hypercalcemic factors (including PMCA2, NCX1, PTH receptors (PTH1R, PTH2R, and PTH3R), and VDR) at 4 days post-fertilization [70]. These results suggest biphasic effects of CT; a CT-mediated decrease of Ca2+ uptake results in short-term hypocalcemia; this may activate hypercalcemic signaling, which would compensate for the Ca2+ imbalance through hypercalcemic hormones and other Ca2+ transporters. This highlights the importance of hormone interactions on Ca2+ homeostasis in zebrafish and mammals.

Regulation of Na+ uptake

Cortisol was also found to play roles in regulating Na+ uptake in zebrafish. Treatment with exogenous cortisol was found to increase Na+ uptake in zebrafish embryos, and this enhancement was blocked by a selective GR antagonist, RU-486 [66]. In contrast, treatment with aldosterone, a selective agonist for MR, had no effect on Na+ uptake, suggesting that cortisol increases Na+ uptake via GR, but not MR signaling [66]. Supporting these pharmacological data, loss-of-function experiments indicated that Na+ uptake was not induced under acidic environments in GR morphants [66]. Overall, it seems that cortisol-GR, but not cortisol-MR, signaling is involved in the regulation of Na+ in zebrafish acclimated to acidic environment [66], although some debates remain. GR loss-of-function experiments did not affect Na+ uptake in zebrafish reared in normal freshwater [66], conflicting with another study demonstrating the role of cortisol-GR signaling in differentiation of all the types of zebrafish ionocytes [21]. In the study by Cruz et al. [21], the same GR morpholinos was found to suppress the differentiation of HR cells (and other types of ionocytes), and a resultant impairment in Na+ uptake in zebrafish is conceivably predicted. This discrepancy needs further clarification in the future.

Neurohormones such as catecholamines also contribute to Na+ uptake in zebrafish. In additional to their role in vasoactive regulation, epinephrine/norepinephrine is known to directly affect gill ionic transport via inhibition by α-adrenergic receptors and stimulation by β receptors (for a detailed review, see [28]). This reverse or biphasic control of α- and β-adrenergic receptors in fish ion regulation was re-confirmed in zebrafish through pharmacological and loss-of-function approaches [69]. Pharmacological screening was performed to identify α- and β-adrenergic receptors with inhibitory and stimulatory roles, respectively, on Na+ uptake in zebrafish embryos [69]. β receptor loss of function impaired Na+ uptake only under acidic or ion poor environment, suggesting that Na+ uptake in zebrafish is regulated via adrenergic receptors [69]. In support of this hypothesis, it was found that HR cells can be labeled with fluorescent propranolol (a β receptor antagonist) and zn-12 (a genetic neuronal marker) [69]. These findings further argue for β-adrenergic regulation of ion transport function in fish gill ionocytes but also raise several questions. Treatment of zebrafish embryos with exogenous epinephrine was reported to suppress Na+ uptake, while norepinephrine treatment stimulated Na+ uptake; this surprising result was attributed to different affinities to the receptors [69]. As in mammals, epinephrine and norepinephrine reach their target tissues (including the gills and skin) in teleosts via both neurotransmission from local sympathetic innervation and by systematic stimulation of chromaffin cells [28]. In mammals, sympathetic nerves participate via α- and β-adrenergic receptors in the complicated regulatory network of several kidney functions associated with body fluid ionic homeostasis [22], and catecholamines and other hormones (including vasopressin, parathyroid, glucagon, and angiotensin II) regulate renal NaCl transport through complex interactions [3, 7]. The conflicting data in zebrafish may perhaps be resolved by taking the interactions between systematic and local catecholamines, different adrenergic receptors, and other hormones, into consideration.

In mammals, renal sympathetic activity induced by β-adrenergic receptor stimulation activates the Na+-K+-2Cl− cotransporter II, NCC, and pendrin [3, 5, 82]. Both HR and NaR ionocytes in zebrafish are innervated by nerve fibers (with zn-12 as a marker; [57, 69]). This raises the possibility that neurohormones may control the transport functions of not only Na+, but also other ions, through regulating various transporters in zebrafish; however, Na+ uptake in zebrafish is reportedly unaffected by serotonin and dopamine [69].

Regulation of other transport functions

Pituitary hormone PRL has long been known to play a major role in hyper-osmoregulation in freshwater teleosts [28]. To understand the role of osmotic homeostasis in the development of PRL-secreting lactotrophs, Liu et al. [79] generated germline transgenic zebrafish expressing red fluorescent protein under the control of PRL regulatory elements (PRL-RFP). They reported that environmental osmotic changes (0.025∼1.0 % of salt concentration) affected PRL cell proliferation in a dopamine-independent manner [79]. The osmotic response in PRL cells was abolished by knockdown of the PRL receptor, and over-expression of PRL decreased the PRL cell mass, suggesting that osmotic feedback on PRL cells is mediated by PRL signaling via the PRL receptor in the pituitary [79]. Other hormones such as atrial natriuretic peptide (ANP) and renin have also been reported to participate in ion regulation in zebrafish. Transcription of the genes encoding ANP and renin in zebrafish embryos was increased in 1/20× (34∼40 μM NaCl) freshwater and decreased in 100× (68∼80 mM NaCl) freshwater [39]. Zebrafish embryos raised in 1/20× freshwater supplemented with 680∼690 μM NaCl presented with severe edema (i.e., hypervolemia) and increased ANP expression (renin expression was unaffected), as compared with those raised in 1/20× freshwater, suggesting that ANP and renin are the volume-regulating and Na+-regulating hormones during freshwater adaptation, respectively [44]. Consequently, PRL/PRL receptor, ANP, and renin were proposed to regulate transport of NaCl and/or other ions in zebrafish during acclimation to freshwater with various ionic compositions [44, 79]. The ionocyte types, transporters, and ions affected by these hormones, and the exact nature of the regulatory mechanisms, remain unknown. The establishment of zebrafish as a model for the identification, functional analysis, and differentiation of ionocytes means there has never been a better time to explore these issues.

Regulation of the proliferation and differentiation of ionocytes

Ionocyte function can be regulated at the level of ionocyte proliferation and differentiation, and hormones are involved at this step. Our understanding of the role of isotocin–neurophysin (Itnp; a homolog of mammalian oxytocin) in fish osmoregulation is limited to the findings of a very few studies into the effects of environmental salinity on isotocin expression and secretion [53, 112]. A recent study in zebrafish demonstrated that isotocin activates the genetic operons for ionocyte progenitor proliferation and differentiation, via transcriptional and translational pathways [19]. Exposing zebrafish embryos to conditions which disturb the body fluid ionic balance (thus triggering a compensatory increase of ionocyte function) was found to increase transcription of the isotocin gene. Further loss- and gain-of-function experiments indicated that Itnp enhances epidermal stem cell proliferation and facilitates ionocyte differentiation by enhancing transcription of Foxi3a [19]. As such, zebrafish isotocin may increase ionocyte density through stimulation of cell proliferation/differentiation, ultimately regulating ionocyte function. On the other hand, the primary mode of action of cortisol on fish ion regulation was proposed to be cytogenic, through mediating the growth and differentiation of ionocytes in the gill epithelium [28]. This hypothesis lacks convincing support because the molecular pathways of proliferation/differentiation of fish ionocytes were unknown until they were recently elucidated in zebrafish (see “Proliferation and differentiation of ionocytes” section). Exogenous cortisol treatment of zebrafish embryos caused a dose-dependent increase in the densities of ionocyte progenitors and mature ionocytes (NaR and HR cells), but had no effect on p63-expressing epidermal stem cells [21]. Cortisol also increased the number of ionocytes in cultured adult zebrafish gills, and this was accompanied by increased expression of Foxi3a/3b; this indicates that cortisol controls ionocyte function by stimulating differentiation of ionocytes, but not epidermal stem cells [21].

In mammals, oxytocin was found to be involved in cell growth and differentiation in many tissues, including heart [34], bone [101], and mammary gland [89]; however, there is no evidence to suggest that oxytocin participates in the differentiation of kidney cells. Oxytocin regulates body fluid homeostasis by stimulating water uptake and simultaneously reducing Na+ reabsorption, which consequently results in a decrease in body fluid osmolarity [38]. Similar to oxytocin, cortisol has also been suggested to be a growth and cell differentiation factor [60, 120]. Moreover, dexamethasone (a synthetic glucocorticoid) was found to increase the mRNA and protein of many ion transporters, including NHE3, NKCC, NCC, and ENaC, in rat kidney [32]. In addition, aldosterone, a mineralocorticoid, contributes to normal renal cell proliferation and differentiation from stem cells, as well as to the development of pathological conditions through dysregulation of renal cell growth in the adult [103]. Taken together, it is possible that oxytocin and cortisol modulate the growth or differentiation of renal tubular cells and thereby regulate transepithelial ion and acid/base transport. Since the specification, differentiation, and patterning of ionocytes have been comprehensively elucidated in zebrafish by taking advantage of the many efficient genetic and molecular tools available, it should also be a suitable model for studying the hormonal control of ionocyte proliferation and differentiation. An understanding of these areas in zebrafish would not only contribute to fish physiological research but also provide insights to aid studies in mammals and other animals.

Conclusions and perspectives

HR, NaR, NCC, and KS ionocytes in zebrafish embryonic skin and gills express distinct sets of ion transporters and thus perform transepithelial H+ secretion/Na+ uptake/NH4 + excretion, Ca2+ uptake, Na+/Cl− uptake, and K+ secretion, respectively. Validation of the proposed zebrafish model requires analysis of the transport kinetics and stoichiometry of several apical transporters (e.g., NCC, pendrin), to understand how these transporters operate in a hypotonic freshwater environment with thermodynamically unfavorable chemical gradients. Basolateral transporters, such as ClC and KCC, await identification and functional analysis. The search for new transporters and transport functions in identified ionocytes or in new types of ionocytes is still in progress, and this may lead to the development of a more comprehensive model. In the model for the molecular pathways of ionocyte development, ionocyte progenitors develop from epidermal stem cells and then differentiate into NaR and HR ionocyte types through a positive regulatory loop involving Foxi3a/-3b and other transcription factors. Although more studies are required to fully elucidate these pathways, the proposed model has helped to pave the way for studies into the role of cell proliferation and differentiation in the functional regulation of ionocytes. Several hormones have been found to participate in the regulatory pathways of ionic homeostasis in zebrafish; detailed studies into the target ion transport pathways, ion transporters, or ionocytes of hormonal actions have been performed, particularly for cortisol, vitamin D, STC-1, CT, and isotocin and their specific receptors, and similar approaches could be applied to other hormones in the future.

The four types of ionocyte in zebrafish are analogous to various renal tubular cells, in terms of their ion transporter expression and function. Zebrafish is highly suited for experimental analysis, in terms of the applicability of molecular tools, ease of in vivo cellular observation and function analysis, and rapid embryonic development, and these advantages enable this model to not only enhance our understanding of body fluid ion homeostasis and hormonal control in fish but to also inform studies on mammals or other animal species, thereby providing insights into related fields. However, the drawbacks of the zebrafish model need to be taken into consideration. While pharmacological and loss-of-function approaches are comparatively easy and efficient methods with which to assay the ion regulatory roles of ion transporters, hormones, and hormone receptors in zebrafish in vivo, the necessary reagents or morpholinos affect whole zebrafish embryos; as such, interpretations of cause and effect should be made cautiously, pending further experiments to tease apart the compensatory responses of different transporters or isoforms and the effects of interactions between systemic and local hormones, different receptors, and other hormones.