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Cation channel activity of mucolipin-1: the effect of calcium

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Abstract

Mucolipidosis type IV (MLIV) is a rare, neurogenetic disorder characterized by developmental abnormalities of the brain, and impaired neurological, ophthalmological, and gastric function. Considered a lysosomal disease, MLIV is characterized by the accumulation of large vacuoles in various cell types. Recent evidence indicates that MLIV is caused by mutations in MCOLN1, the gene that encodes mucolipin-1 (ML1), a 65-kDa protein showing sequence homology and topological similarities with polycystin-2 and other transient receptor potential (TRP) channels. In this report, our observations on the channel properties of ML1, and molecular pathophysiology of MLIV are reviewed and expanded. Our studies have shown that ML1 is a multiple sub-conductance, non-selective cation channel. MLIV-causing mutations result in functional differences in the channel protein. In particular, the V446L and ΔF408 mutations retain channel function but have interesting functional differences with regards to pH dependence and Ca2+ transport. While the wild-type protein is inhibited by Ca2+ transport, mutant ML1 is not. Atomic force microscopy imaging of ML1 channels shows that changes in pH modify the aggregation and size of the ML1 channels, which has an impact on vesicular fusogenesis. The new evidence provides support for a novel role of ML1 cation channels in vesicular acidification and normal endosomal function.

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Author notes

  1. Wolfgang H. Goldmann

    Present address: Center for Medical Physics and Technology, Friedrich Alexander University, Erlangen-Nuernberg, Germany

Authors and Affiliations

  1. Renal Unit, Massachusetts General Hospital East, 149 13th Street, Charlestown, 02129, MA, USA

    Horacio F. Cantiello, Wolfgang H. Goldmann & Malay K. Raychowdhury

  2. Department of Medicine, Harvard Medical School, Boston, MA, USA

    Horacio F. Cantiello, Wolfgang H. Goldmann & Malay K. Raychowdhury

  3. Laboratorio de Canales Iónicos, Departamento de Fisicoquímica y Química Analítica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina

    Horacio F. Cantiello, Nicolás Montalbetti, Silvia González-Perrett & Gustavo A. Timpanaro

  4. Physics Department, Boston University, Boston, MA, USA

    Bernard Chasan

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  1. Horacio F. Cantiello
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  2. Nicolás Montalbetti
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Correspondence to Horacio F. Cantiello.

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Cantiello, H.F., Montalbetti, N., Goldmann, W.H. et al. Cation channel activity of mucolipin-1: the effect of calcium. Pflugers Arch - Eur J Physiol 451, 304–312 (2005). https://doi.org/10.1007/s00424-005-1448-9

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  • DOI: https://doi.org/10.1007/s00424-005-1448-9

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