Abstract
Aerosol gene transfer of endothelial nitric oxide synthase (eNOS) and inducible NOS (iNOS) to rat lungs increased NOS expression and activity, and prevented hypoxic pulmonary vasoconstriction (HPV) in vivo. Hereby, we examined the effect of eNOS and iNOS aerosol gene transfer on the endothelium-dependent relaxation (EDR) and on acute HPV in isolated rat pulmonary arteries. Changes in isometric forces were recorded in organ baths for large conduit arteries (diameter 1.8±0.1 mm) and in a wire myograph for small resistance arteries (258±35 μm). Male Wistar rats were randomly aerosolized with adenovirus (Ad) encoding β-galactosidase (control), eNOS, or iNOS. Four days later, exhaled nitric oxide was measured, NOS expression within rat lungs was evaluated by quantitative real-time polymerase chain reaction and immunohistochemistry, vasoconstricting agonist and acetylcholine concentration response curves were generated, and the time course of HPV was recorded. Human eNOS and murine iNOS were expressed within rat lung tissue mostly in parenchyma and endothelial cells. Large arteries isolated from Ad-i, eNOS-aerosolized rats developed lower agonist-induced tension than those of control rats. The first and second contractions of the HPV were smaller in the Ad-i, eNOS-aerosolized rats. Contractions were modestly, but significantly and inversely, related to exhaled NO. Agonist- and hypoxia-induced contractions were even more reduced after eNOS aerosolization. There was no significant effect on EDR and no notable difference between small and large vessels. We conclude that adenovirus (Ad)-mediated NOS gene transfer can counteract both pharmacologically and hypoxia-induced increases in pulmonary vascular tone in isolated rat pulmonary arteries. eNOS seems as efficient as iNOS in regulating pulmonary vascular tone.
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Aaronson PI, Robertson TP, Ward JP (2002) Endothelium-derived mediators and hypoxic pulmonary vasoconstriction. Respir Physiol Neurobiol 132:107–120
Archer SL, Huang JMC, Reeve HL, Hampl V, Tolarova S, Michelakis E, Weir EK (1996) Differential distribution of electrophysiologically distinct myocytes in conduit and resistance arteries determines their response to nitric oxide and hypoxia. Circ Res 78:431–442
Budts W, Pokreisz P, Nong Z, Van Pelt N, Gillijns H, Gerard R, Lyons R, Collen D, Bloch K, Janssens S (2000) Aerosol gene transfer with inducible nitric oxide synthase reduces hypoxic pulmonary hypertension and pulmonary vascular remodeling in rats. Circulation 102:2880–2885
Champion HC, Bivalacqua TJ, D’Souza FM, Ortiz LA, Jeter JR, Toyoda K, Heistad DD, Hyman AL, Kadowitz PJ (1999) Gene transfer of endothelial nitric oxide synthase to the lung of the mouse in vivo. Effect on agonist-induced and flow-mediated vascular responses. Circ Res 84:1422–1432
Champion HC, Bivalacqua TJ, Greenberg SS, Giles TD, Hyman AL, Kadowitz PJ (2002) Adenoviral gene transfer of endothelial nitric-oxide synthase (eNOS) partially restores normal pulmonary arterial pressure in eNOS-deficient mice. Proc Natl Acad Sci U S A 99:13248–13253
Channick RN, Newhart JW, Johnson FW, Williams PJ, Auger WR, Fedullo PF, Moser KM (1996) Pulsed delivery of inhaled nitric oxide to patients with primary pulmonary hypertension: an ambulatory delivery system and initial clinical tests. Chest 109:1545–1549
Christman BW, McPherson CD, Newman JH, King GA, Bernard GR, Groves BM, Loyd JE (1992) An imbalance between the excretion of thromboxane and prostacyclin metabolites in pulmonary hypertension. New Engl J Med 327:70–75
Demiryurek AT, Wadsworth RM, Kane KA, Peacock AJ (1993) The role of endothelium in hypoxic constriction of human pulmonary artery rings. Am Rev Respir Dis 147:283–290
Emery CJ, Teng GQ, Liu X, Barer GR (2003) Vasoreactions to acute hypoxia, whole lungs and isolated vessels compared: modulation by NO. Respir Physiol Neurobiol 134:115–129
Fagan KA, Tyler RC, Sato K, Fouty BW, Morris KG Jr, Huang PL, McMurtry IF, Rodman DM (1999) Relative contributions of endothelial, inducible, and neuronal NOS to tone in the murine pulmonary circulation. Am J Physiol 277:L472–L478
Giaid A, Saleh D (1995) Reduced expression of endothelial nitric oxide synthase in the lungs of patients with pulmonary hypertension. New Engl J Med 333:214–221
Hampl V, Herget J (2000) Role of nitric oxide in the pathogenesis of chronic pulmonary hypertension. Physiol Rev 80:1337–1372
Herz J, Gerard RD (1993) Adenovirus-mediated transfer of low density lipoprotein receptor gene acutely accelerates cholesterol clearance in normal mice. Proc Natl Acad Sci U S A 90:2812–2816
Janssens SP, Bloch KD, Nong Z, Gerard RD, Zoldhelyi P, Collen D (1996) Adenoviral-mediated transfer of the human endothelial nitric oxide synthase gene reduces acute hypoxic pulmonary vasoconstriction in rats. J Clin Invest 98:317–324
Jin N, Packer S, Rhoades RA (1992) Pulmonary arterial hypoxic contraction: signal transduction. Am J Physiol 263:L73–L78
Kouyoumdjian C, Adnot S, Levame M, Eddahibi S, Bousbaa H, Raffestin B (1994) Continuous inhalation of nitric oxide protects against development of pulmonary hypertension in chronically hypoxic rats. J Clin Invest 94:578–584
Le Cras TD, Xue C, Rengasamy A, Johns RA (1996) Chronic hypoxia upregulates endothelial and inducible NO synthase gene and protein expression in rat lung. Am J Physiol 270:L164–L170
Leach RM, Twort CHC, Cameron IR, Ward PT (1992) A comparison of the pharmacological and mechanical properties in vitro of large and small pulmonary arteries of the rat. Clin Sci 82:55–62
Leach RM, Sheehan DW, Chacko VP, Sylvester JT (2000) Energy state, pH, and vasomotor tone during hypoxia in precontracted pulmonary and femoral arteries. Am J Physiol 278:L294–L304
Lemarchand P, Jones M, Danel C, Yamada I, Mastrangeli A, Crystal RG (1994) In vivo adenovirus-mediated gene transfer to lungs via pulmonary artery. J Appl Physiol 76:2840–2845
Muller DW, Gordon D, San H, Yang Z, Pompili VJ, Nabel GJ, Nabel EG (1994) Catheter-mediated pulmonary vascular gene transfer and expression. Circ Res 75:1039–1049
Mulvany MJ, Halpern W (1977) Contractile properties of small arterial resistance vessels in spontaneously hypertensive and normotensive rats. Circ Res 41:19–26
Nabel EG, Yang Z, Muller D, Chang AE, Gao X, Huang L, Cho KJ, Nabel GJ (1994) Safety and toxicity of catheter gene delivery to the pulmonary vasculature in a patient with metastatic melanoma. Hum Gene Ther 5:1089–1094
Ozaki M, Marshall C, Amaki Y, Marshall BE (1998) Role of wall tension in hypoxic responses of isolated rat pulmonary arteries. Am J Physiol 275:L1069–L1077
Priest RM, Hucks D, Ward JP (1997) Noradrenaline, beta-adrenoceptor mediated vasorelaxation and nitric oxide in large and small pulmonary arteries of the rat. Br J Pharmacol 122:1375–1384
Quarck R, De Geest B, Stengel D, Mertens A, Lox M, Theilmeier G, Michiels C, Raes M, Bult H, Collen D, Van Veldhoven P, Ninio E, Holvoet P (2001) Adenovirus-mediated gene transfer of human platelet-activating factor-acetylhydrolase prevents injury-induced neointima formation and reduces spontaneous atherosclerosis in apolipoprotein E-deficient mice. Circulation 103:2495–2500
Rodman DM, San H, Simari R, Stephan D, Tanner F, Yang Z, Nabel GJ, Nabel EG (1997) In vivo gene delivery to the pulmonary circulation in rats: transgene distribution and vascular inflammatory response. Am J Respir Cell Mol Biol 16:640–649
Shaul PL, North AJ, Brannon TS, Ujiie K, Wells LB, Nisen PA, Lowenstein CJ, Snyder SH, Star RA (1995) Prolonged in vivo hypoxia enhances nitric oxide synthase type I and type III gene expression in adult rat lung. Am J Respir Cell Mol Biol 13:167–174
Stewart DJ, Levy RD, Cernacek P, Langleben D (1991) Increased plasma endothelin-1 in pulmonary hypertension: maker or mediator of disease? Ann Intern Med 114:464–469
Von Euler US, Liljestrand G (1946) Observations on the pulmonary arterial blood pressure in the cat. Acta Physiol Scand 12:301–320
Weir EK, Archer SL (1995) The mechanism of acute hypoxic pulmonary vasoconstriction: the tale of two channels. FASEB J 9:183–189
Woodmansey PA, Zhang F, Channer KS, Morice AH (1993) Vasodilatory action of the calcium antagonist amlodipine on large and resistance pulmonary arteries from normoxic and chronically hypoxic rats. Clin Sci (Lond) 85:361–366
Woodmansey PA, Zhang F, Channer KS, Morice AH (1995) Effect of the calcium antagonist amlodipine on the two phases of hypoxic pulmonary vasoconstriction in rat large and small isolated pulmonary arteries. J Cardiovasc Pharmacol 25:324–329
Xue C, Rengasamy A, Le Cras TD, Koberna PA, Dailey GC, Johns RA (1994) Distribution of NOS in normoxic vs. hypoxic rat lung: upregulation of NOS by chronic hypoxia. Am J Physiol 267:L667–L678
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The authors thank M. Cauberghs, N. Van Pelt, and H. Gillijns for expert technical assistance. This work was supported by the National Fund for Scientific Research (FWO) grant 1.5650.98N.
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Jiang, L., Quarck, R., Janssens, S. et al. Effect of adenovirus-mediated gene transfer of nitric oxide synthase on vascular reactivity of rat isolated pulmonary arteries. Pflugers Arch - Eur J Physiol 452, 213–221 (2006). https://doi.org/10.1007/s00424-005-0028-3
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DOI: https://doi.org/10.1007/s00424-005-0028-3