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The impact of ischemic preconditioning on hemodynamic, biochemical and inflammatory alterations induced by intra-abdominal hypertension: an experimental study in a porcine model

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Abstract

Purpose

Intra-abdominal hypertension (IAH) has several pathophysiologic implications on human organs and systems. The aim of this experimental study was to investigate whether ischemic preconditioning (IP), namely the application of IAH for a small period of time prior to establish pneumoperitoneum, can attenuate the hemodynamic, biochemical and inflammatory alterations observed during IAH.

Methods

Twenty-four pigs were divided into three groups: group A (control group), group B (pneumoperitoneum of 30 mmHg) and group C (ischemic preconditioning, consisting of pneumoperitoneum of 25 mmHg for 15 min and subsequent pneumoperitoneum of 30 mmHg). Hemodynamic (central venous pressure, cardiac index, mean arterial pressure, heart rate, stroke volume index, systemic vascular resistance index, global end-diastolic index, intrathoracic blood index and extravascular lung water index), biochemical (serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvate transaminase (SGPT), alkaline phosphatase (ALP), γ-glutamyl transpeptidase (γ-GT), urea and creatinine) and inflammatory (tumour necrosis factor-α, interleukin (IL)-6, IL-10 and C-reactive protein) parameters were measured.

Results

(a) Hemodynamics: The increase of central venous pressure monitoring and heart rate and the decrease of cardiac index, mean arterial pressure, stroke volume index, global end-diastolic volume index and intrathoracic blood volume index with the establishment of pneumoperitoneum were attenuated by IP. Systemic vascular resistance index and extravascular lung water were not affected. (b) Urea significantly increased with the pneumoperitoneum. IP, however, attenuated this effect. Οther biochemical parameters (SGOT, SGPT, ALP, γ-GT and creatinine) had a similar upward trend during IAH, which was reversed with IP. (c) Inflammatory parameters: CRP was increased with pneumoperitoneum, an effect that was attenuated with the application of IP. Νo significant differences were observed for interleukins.

Conclusions

Ischemic preconditioning seems to attenuate the pathophysiologic alterations of several hemodynamic, biochemical and inflammatory parameters observed during IAH.

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Acknowledgments

This work was supported by the Special Account for Research of the National and Kapodistrian University of Athens.

Conflicts of interest

None.

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Authors and Affiliations

  1. First Department of Anesthesiology, Aretaieion University Hospital, 76 Vassilisis Sofia’s Ave, 11528, Athens, Greece

    Alexandra Avraamidou & Eriphili Argyra

  2. Second Department of Surgery, Aretaieion University Hospital, 76 Vassilisis Sofia’s Ave, 11528, Athens, Greece

    Athanasios Marinis, Spyridon Asonitis, Georgios Polymeneas & Dionysios Voros

  3. Laboratory of Experimental Surgery and Surgical Research “NS Christeas” (LESSR), Medical School of Athens, University of Athens, Athens, Greece

    Despoina Perrea

  4. 50-52 Akti Themistokleous St., 18537, Piraeus, Greece

    Alexandra Avraamidou

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  1. Alexandra Avraamidou
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Correspondence to Alexandra Avraamidou.

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Ethical standards

This study conforms to our institutional standards and is under the appropriate license of the veterinary authorities and in adherence to National and European regulations for animal studies.

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Avraamidou, A., Marinis, A., Asonitis, S. et al. The impact of ischemic preconditioning on hemodynamic, biochemical and inflammatory alterations induced by intra-abdominal hypertension: an experimental study in a porcine model. Langenbecks Arch Surg 397, 1333–1341 (2012). https://doi.org/10.1007/s00423-012-0977-x

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  • DOI: https://doi.org/10.1007/s00423-012-0977-x

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