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Whole-body vibration and resistance exercise prevent long-term hindlimb unloading-induced bone loss: independent and interactive effects

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Abstract

Skeletal unloading induced by disuse or immobilization causes a decrease in bone mass and strength. We investigated the relationship between whole-body vibration (WBV) and resistance exercise (RE) in preventing bone loss induced by 8-week hindlimb unloading in young male rats. Sixty male Wistar rats were assigned randomly to 6 groups: age-matched control group (CON, n = 10), hindlimb unloading group (HU, n = 10), hindlimb unloading + standing group (HU + ST, n = 10), hindlimb unloading + WBV group (HU + WBV, n = 10), hindlimb unloading + RE group (HU + RE, n = 10) and hindlimb unloading + WBV + RE group (HU + WBV + RE, n = 10). After 8-week hindlimb unloading, micro-CT scanning and three-point bending test were performed in the femur. Sera were collected for analysis of bone formation and resorption markers. Compared with HU group, WBV, RE and the combination of WBV and RE (WBV + RE) significantly improved (P < 0.01) one repetition maximum (1RM) (expressed as the percentage change from baseline, HU: −23%, HU + WBV: 21%, HU + RE: 48%, HU + WBV + RE: 51%), and maintained (P < 0.05) cancellous volumetric bone mineral density (vBMD) and trabecular structure. No difference of cortical vBMD was found among all groups (P > 0.05). WBV had no effects on biomechanical properties of the femur diaphysis (P > 0.05). RE and WBV + RE significantly increased maximum load and cross-sectional moment of inertia of the femur diaphysis in hindlimb unloading rats (P < 0.05). There was an interaction between WBV and RE in improving cancellous bone. These results demonstrate that WBV and RE interactively maintain cancellous structure and vBMD, and independently partially mitigate the reduction of bone strength in long-term hindlimb unloading rats.

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Acknowledgments

We would like to thank Professor Ruiping She for her contribution to this research. This work was supported by Advanced Space Medico-Engineering Research Project of China, grant NO.SJ200802.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Aerospace Biodynamics, School of Aerospace Medicine, Fourth Military Medical University, 710032, Xi’an, China

    Zhili Li

  2. State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, 100094, Beijing, China

    Zhili Li, Cheng Tan, Huijuan Wang, Wenjuan Chen, Yu Zhu, Honglei Ma, Honghui Yang, Shizhong Jiang, Desheng Wang, Linjie Wang & Guohua Tang

  3. Department of Laboratory Medicine, Peking University Third Hospital, 100191, Beijing, China

    Yonghua Wu

  4. Department of Veterinary Pathology, Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, 100193, Beijing, China

    Ye Ding

  5. Cedars-Sinai Heart Institute, Los Angeles, CA, 90048, USA

    Wenbin Liang

  6. Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, 710032, Xi’an, China

    Jun Wang

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  1. Zhili Li
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Correspondence to Zhili Li.

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Communicated by Susan A. Ward.

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Li, Z., Tan, C., Wu, Y. et al. Whole-body vibration and resistance exercise prevent long-term hindlimb unloading-induced bone loss: independent and interactive effects. Eur J Appl Physiol 112, 3743–3753 (2012). https://doi.org/10.1007/s00421-012-2355-3

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