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Proteomic analysis of mitochondrial proteins in cardiomyocytes from rats subjected to intermittent hypoxia

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Abstract

Intermittent hypoxia (IH) markedly enhances cardiac tolerance against ischemia/reperfusion injury, but its mechanism and molecular basis remain unclear. For exploring the expression of mitochondrial proteins induced by IH, two-dimensional electrophoresis and Thermo Finnigan LTQ mass spectrometer (MS) were applied. After comparing the protein profiles of myocardial mitochondria between IH and normoxic hearts, 14 protein spots were found to be altered more than threefold between the two groups, 11 of which were identified by Finnigan LTQ MS. Among these 11 proteins, 9 were involved in energy metabolism, including 7 that were increased after IH. The latter were identified as aldehyde dehydrogenase, methylmalonate-semialdehyde dehydrogenase, ATP synthase β chain, mitochondrial aconitase, malate dehydrogenase, electron transfer flavoprotein α subunit and sirtuin 5. Two other proteins, ubiquinol-cytochrome C reductase iron-sulfur subunit and aspartate aminotransferase, were decreased after IH. Biochemical tests for energy metabolism in mitochondria supported the proteomic results. IH exposure also increased the expression of a molecular chaperone—heat shock protein 60 and an antioxidant protein, peroxiredoxin 5. These findings will provide clues for understanding the mechanism of IH-induced cardiac protection and may lead to the development of interventional strategies designed to utilize the advantages of IH clinically.

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Acknowledgments

We thank the Research Center for Proteome Analysis, Key Laboratory of Systems Biology, Shanghai Institutes for Biological Sciences for excellent technical assistance. The authors wish to thank Drs. John Tyberg, Michael A. Laflamme and Jun Zhou for critical reading of the manuscript. This study was supported in part by the grants from the National Natural Science Foundation (No. 30393130) and the National Basic Research Program of China “973” (No. 2006CB504100).

Conflict of interest

The authors have no competing interests or financial ties to disclose.

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Author notes

  1. Wei-Zhong Zhu

    Present address: Department of Pathology, University of Washington, Seattle, WA, 98109, USA

Authors and Affiliations

  1. Physiological Laboratory of Hypoxia, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320# Yue Yang Road, 200031, Shanghai, People’s Republic of China

    Wei-Zhong Zhu, Xiu-Feng Wu & Zhao-Nian Zhou

  2. Department of Physiology, Hebei Medical University, 050017, Shijiazhuang, China

    Yi Zhang

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  1. Wei-Zhong Zhu
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  2. Xiu-Feng Wu
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Correspondence to Zhao-Nian Zhou.

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Communicated by Keith Phillip George.

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Zhu, WZ., Wu, XF., Zhang, Y. et al. Proteomic analysis of mitochondrial proteins in cardiomyocytes from rats subjected to intermittent hypoxia. Eur J Appl Physiol 112, 1037–1046 (2012). https://doi.org/10.1007/s00421-011-2050-9

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  • DOI: https://doi.org/10.1007/s00421-011-2050-9

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