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Four weeks one-leg training and high fat diet does not alter PPARα protein or mRNA expression in human skeletal muscle

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Abstract

Fatty acid metabolism is influenced by training and diet with exercise training mediating this through activation of nuclear hormone receptor peroxisome proliferator-activated receptor α (PPARα) in skeletal muscle. This study investigated the effect of training and high fat or normal diet on PPARα expression in human skeletal muscle. Thirteen men trained one leg (T) four weeks (31.5 h in total), while the other leg (UT) served as control. During the 4 weeks six subjects consumed high fat (FAT) diet and seven subjects maintained a normal (CHO) diet. Biopsies were obtained from vastus lateralis muscle in both legs before and after training. After the biopsy, one-leg extension exercise was performed in random order with both legs 30 min at 95% of workload max. A training effect was evident as citrate synthase activity increased (P < 0.05) by 15% in the trained, but not the control leg in both groups. During exercise respiratory exchange ratio was lower in FAT (0.86 ± 0.01, 0.83 ± 0.01, mean ± SEM) than CHO (0.96 ± 0.02, 0.94 ± 0.03) and in UT than T legs, respectively. The PPARα protein (144 ± 44, 104 ± 28, 79 ± 15, 79 ± 14, % of pre level) and PPARα mRNA (69 ± [2, 2], 78 ± [7, 6], 92 ± [22, 18], 106 ± [21, 18], % of pre level, geometric mean ± SEM) expression remained unchanged by diet and training in FAT (UT, T) and CHO (UT, T), respectively. After the training and diet CS, HAD, PPARα, UCP2, UCP3 and mFABP mRNA content remained unchanged, whereas GLUT4 mRNA was lower in both groups and LDHA mRNA was lower (P < 0.05) only in FAT. In conclusion: 4 weeks one leg knee extensor training did not affect PPARα protein or mRNA expression. Furthermore, higher fat oxidation during exercise after fat rich diet was not accompanied by an increased PPARα protein or mRNA expression after 4 weeks.

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Acknowledgments

Erik A. Richter is thanked for performing the muscle biopsies. The skilled technical assistance of Irene Bech Nielsen, Jan Arvidsen and Jonas Treebak is acknowledged. The study was supported by grants from The Ministry of Culture Committee on Sports Research J.nr. N2000-10-033), Team Danmark Research funding and the Danish National Research Foundation (#504-14).

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Authors and Affiliations

  1. Copenhagen Muscle Research Centre, Department Medical Physiology, Panum Institute building 12, University of Copenhagen, Blegdamsvej 3, 2200 N, Copenhagen, Denmark

    J. W. Helge & M. Willer

  2. Health and Sports Science, School of Medical Sciences, University of New South Wales, Sydney, Australia

    D. Bentley

  3. Department of Medical Biochemistry and Genetics, University of Copenhagen, Copenhagen, Denmark

    P. Schjerling

  4. Exercise Metabolism Research Group, Department of Kinesiology, McMaster University, Hamilton, Canada

    M. J. Gibala

  5. Institute of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark

    J. Franch

  6. Novo Nordisk A/S, Novo Nordisk Park, Måløv, Denmark

    J. R. Daugaard

  7. Department of Molecular Muscle Biology, State Hospital, Copenhagen, Denmark

    P. Schjerling, M. A. Tapia-Laliena & J. L. Andersen

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  1. J. W. Helge
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  9. J. L. Andersen
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Correspondence to J. W. Helge.

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Helge, J.W., Bentley, D., Schjerling, P. et al. Four weeks one-leg training and high fat diet does not alter PPARα protein or mRNA expression in human skeletal muscle. Eur J Appl Physiol 101, 105–114 (2007). https://doi.org/10.1007/s00421-007-0479-7

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