Abstract
The aim of the present study was to examine the differences in fat oxidation between endurance trained (ET) and untrained (UT) women. Eight ET and nine UT women performed a progressive cycle ergometer test until exhaustion. The rate of fat oxidation was similar at low work rates (≤90 W) but was 80–200% higher in ET subjects at 120–180 W. When related to relative exercise intensity, the fat oxidation was similar in the low-intensity domain (≤40% VO2max), but higher in the ET subjects both at moderate intensities (45–60% VO2max; +22% vs. UT) and at high intensities (65–80% VO2max; +35% vs. UT). There was no difference in the maximal fat oxidation rates between the trained and untrained women. The relative exercise intensity that elicited the highest rate of fat oxidation (Fatmax) was 56 ± 3% and 53 ± 2% VO2max in ET and UT women, respectively (NS). In biopsies from m. vastus lateralis, the activity of the enzymes citrate synthase, β-hydroxy acyl CoA dehydrogenase (HAD), and hormone sensitive lipase was higher in the ET subjects. The HAD activity correlated significantly with fat oxidation at moderate and high intensities. We conclude that the ET women had a higher fat oxidation at moderate- and high-exercise intensities both at same relative and at absolute intensity compared with the UT women. The HAD activity and fat oxidation rates were highly correlated indicating that training-induced adaptation in muscle fat oxidative capacity is an important factor for enhanced fat oxidation. Interestingly, maximal fat oxidation occurred at the same exercise intensity.
Similar content being viewed by others
References
Achten J, Jeukendrup AE (2003) Maximal fat oxidation during exercise in trained men. Int J Sports Med 24:603–608
Achten J, Gleeson M, Jeukendrup AE (2002) Determination of the exercise intensity that elicits maximal fat oxidation. Med Sci Sports Exerc 34:92–97
Achten J, Venables MC, Jeukendrup AE (2003) Fat oxidation rates are higher during running compared with cycling over a wide range of intensities. Metabolism 52:747–752
Andersen JL, Aagaaard P (2000) Myosin heavy chain IIX overshoot in human skeletal muscle. Muscle Nerve 23:1095–1104
Bergman BC, Brooks GA (1999) Respiratory gas-exchange ratios during graded exercise in fed and fasted trained and untrained men. J Appl Physiol 86:479–487
Bergman BC, Butterfield GE, Wolfel EE, Casazza GA, Lopaschuk GD, Brooks GA (1999) Evaluation of exercise and training on muscle lipid metabolism. Am J Physiol 276:E106–E117
Berthon PM, Howlett RA, Heigenhauser GJF, Spriet LL (1998) Human skeletal muscle carnitine palmitoyltransferase I activity determined in isolated intact mitochondria. J Appl Physiol 85:148–153
Bezaire V, Heigenhauser GJF, Spriet LL (2004) Regulation of CPT1 activity in intermyofibrillar and subsarcolemmal mitochondria isolated from human and rat skeletal muscle. Am J Physiol 286:E85–E91
Carter SL, Rennie C, Tarnopolsky MA (2001) Substrate utilization during endurance exercise in men and women after endurance training. Am J Physiol 280:E898–E907
Coyle EF, Jeukendrup AE, Wagenmakers AJ, Saris WH (1997) Fatty acid oxidation is directly regulated by carbohydrate metabolism during exercise. Am J Physiol 273:E268–E275
Enevoldsen LH, Stallknecht B, Langfort J, Petersen LN, Holm C, Ploug T, Galbo H (2001) The effect of exercise training on hormone-sensitive lipase in rat intra-abdominal adipose tissue and muscle. J Physiol 536:871–877
Frayn KN (1983) Calculation of substrate oxidation rates in vivo from gaseous exchange. J Appl Physiol 55:628–634
Friedlander AL, Casazza GA, Horning MA, Buddinger TF, Brooks GA (1998a) Effects of exercise intensity and training on lipid metabolism in young women. Am J Physiol 38:E853–E863
Friedlander AL, Casazza GA, Horning MA, Huie MJ, Piacentini MF, Trimmer JK, Brooks GA (1998b) Training-induced alterations of carbohydrate metabolism in women: women respond differently from men. J Appl Physiol 85:1175–1186
Helge JW, Kiens B (1997) Muscle enzyme activity in man: role of substrate availability and training. Am J Physiol Regul Integr Comp Physiol 272:R1620–R1624
Horton TJ, Pagliassotti MJ, Hobbs K, Hill JO (1998) Fuel metabolism in men and women during and after long-duration exercise. J Appl Physiol 85:1823–1832
Horton TJ, Miller EK, Glueck D, Tench K (2002) No effect of menstrual cycle phase on glucose kinetics and fuel oxidation during moderate-intensity exercise. Am J Physiol 282:E752–E762
Jensen MD, Levine J (1998) Effects of oral contraceptives in free fatty acid metabolism in women. Metabolism 47:280–284
Karlsson J, Saltin B (1971) Diet, muscle glycogen, and endurance performance. J Appl Physiol 31:203–206
Kiens B (1997) Effect of endurance training on fatty acid metabolism: local adaptations. Med Sci Sports Exerc 29:640–645
Klein S, Coyle EF, Wolfe RR (1994) Fat metabolism during low-intensity exercise in endurance-trained and untrained men. Am J Physiol 267:E934–E940
Langfort J, Ploug T, Ihlemann J, Saldo M, Holm C, Galbo H (1999) Expression of hormone-sensitive lipase and its regulation by adrenaline in skeletal muscle. Biochem J 340:459–465
Langfort J, Ploug T, Donsmark M, Gorski J, Galbo H (2002) Training enhances contraction-mediated hormone-sensitive lipase activation in rat soleus muscle. In: M. Kkoskolou, Geladas N, Klissouras V (eds) 7th Annual Congress of the ECSS, Athens, p 1137
Langfort J, Donsmark M, Ploug T, Holm C, Galbo H (2003) Hormone-sensitive lipase in skeletal muscle: regulatory mechanism. Acta Physiol Scand 178:397–403
van Loon LJ (2004) Intramyocellular triacylglycerol as a substrate source during exercise. Proc Nutr Soc 63:301–307
van Loon LJC, Greenhaff PL, Constantin-Teodosiu D, Saris WHM, Wagenmakers AJM (2001) The effects of increasing exercise intensity on muscle fuel utilisation in humans. J Physiol 536:295–304
Lowry OH, Passonneau JV (1972) A flexible system of enzymatic analysis. Academic, New York, pp 237–249
Martin WH 3rd, Dalsky GP, Hurley BF, Matthews DE, Bier DM, Hagberg JM, Rogers MA, King DS, Holloszy JO (1993) Effect of endurance training on plasma free fatty acid turnover and oxidation during exercise. Am J Physiol 265:E708–E714
Montain SJ, Hopper MK, Coggan AR, Coyle EF (1991) Exercise metabolism at different time intervals after a meal. J Appl Physiol 70:882–888
Nordby P, Saltin B, Helge JW (2006) Whole-body fat oxidation determined by graded exercise and indirect calorimetry: a role for muscle oxidative capacity? Scand J Med Sci Sports 16(3):209–214
Phillips SM, Green HJ, Tarnopolsky MA, Heigenhauser GJ, Grant SM (1996) Progressive effect of endurance training on metabolic adaptations in working skeletal muscle. Am J Physiol 270:E265–E272
Roepstorff C, Steffensen CH, Madsen M, Kiens B (2002) Gender differences in substrate utilization during submaximal exercise in endurance-trained subjects. Am J Physiol 282:E435–E447
Romijn JA, Coyle EF, Sidossis LS, Gastaldelli A, Horowitz JF, Endert E, Wolfe RR (1993) Regulation of endogenous fat and carbohydrate metabolism in relation to exercise intensity and duration. Am J Physiol 265:E380–E391
Sahlin K (1990) Muscle carnitine metabolism during incremental dynamic exercise in humans. Acta Physiol Scand 138:259–262
Saltin B, Gollnick PD (1983) Skeletal muscle adaptability: significance for metabolism and performance. In: Peachey LD, Adrian RH, Geiger SR (eds) The handbook of physiology. skeletal muscle. Williams & Wilkins, Baltimore, pp 555–631
Spriet LL, Watt MJ (2003) Regulatory mechanisms in the interaction between carbohydrate and lipid oxidation during exercise. Acta Physiol Scand 178:443–452
Starritt EC, Howlett RA, Heigenhauser GJF, Spriet LL (2000) Sensitivity of CPT I to malonyl-CoA in trained and untrained human skeletal muscle. Am J Physiol 278:E462–E468
Suh SH, Casazza GA, Horning MA, Miller BF, Brooks GA (2002) Luteal and follicular glucose fluxes during rest and exercise in 3-h postabsorptive women. J Appl Physiol 93:42–50
Suh SH, Casazza GA, Horning MA, Miller BF, Brooks GA (2003) Effects of oral contraceptives on glucose flux and substrate oxidation rates during rest and exercise. J Appl Physiol 94:285–294
Venables MC, Achten J, Jeukendrup AE (2005) Determinants of fat oxidation during exercise in healthy men and women: a cross-sectional study. J Appl Physiol 98:160–167
Zehnder M, Ith M, Kreis R, Saris W, Boutellier U, Boesch C (2005) Gender-specific usage of intramyocellular lipids and glycogen during exercise. Med Sci Sports Exerc 37:1517–1524
Acknowledgments
We would like to thank all the subjects who committed to participate in the study. We are grateful for the excellent technical assistance in the laboratory by Benthe Jørgensen.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Stisen, A.B., Stougaard, O., Langfort, J. et al. Maximal fat oxidation rates in endurance trained and untrained women. Eur J Appl Physiol 98, 497–506 (2006). https://doi.org/10.1007/s00421-006-0290-x
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00421-006-0290-x