Abstract
This study tested whether regional differences in oxygenation status could result from differences in muscle fiber architecture. Architectural properties, oxygen supply, and consumption in the medial head of the gastrocnemius muscle (GM) were determined in vivo in six men using B-mode ultrasound and functional near infrared (NIR) imaging devices. Fascicle length, fascicle angle, NIR-O2 saturation (deoxygenated Hb or oxygenated Hb), and NIR-blood volume (sum of deoxygenated and oxygenated Hb) were obtained in the distal and proximal portions of the GM at rest and during contraction. Exercise consisted of 2 min of standing plantar flexion at 1 Hz with an additional load of 50% of each subject’s weight. Plantar flexion produced larger decreases (Δ: difference between rest and exercise values) in NIR-O2 saturation [mean saturation (SD) of 0.14 (0.05) vs 0.07 (0.04) optical density units] and NIR-blood volume [mean saturation (SD) of –0.23 (0.08) vs −0.13 (0.04) optical density units] in the distal compared with the proximal portion (P<0.05 for all comparisons). It also produced larger changes (Δ) in fascicle length [mean length (SD) of –16.5 (4.7) vs −8.2 (4.2) mm] and fascicle angle (mean angle (SD) of 10.8 (1.4)° vs 3.9 (2.1)°] in the distal compared with the proximal portion (P<0.05 for all comparisons). There were significant correlations between ΔNIR-O2 saturation and Δfascicle length (r=−0.84, P<0.05), and between ΔNIR-O2 saturation and Δfascicle angle (r=−0.90, P<0.05), between ΔNIR-blood volume and Δfascicle length (r=0.91, P<0.05), between ΔNIR-blood volume and Δfascicle angle (r=−0.85, P<0.05). In conclusion, the plantar flexion exercise produced regional differences in oxygenation status consistent with regional differences in muscle architecture.
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References
Ameredes BT, Provenzano MA (1997) Regional intramuscular pressure development and fatigue in the canine gastrocnemius muscle in situ. J Appl Physiol 83:1867–1876
Barcroft H, Millen JLE (1993) Blood flow through muscle during sustained contractions. J Appl Physiol 74:1499–1503
Belardinelli R, Barstow T, Porszasz J, Wasserman K (1995) Skeletal muscle oxygenation during constant work rate exercise. Med Sci Sports Exerc 27:512–519
Chance B, Dait TM, Chang C, Hamaoka T, Hagerman F (1992) Recovery from exercise-induced desaturation in the quadriceps muscle of elite competitive rowers. Am J Physiol 262:C766-C775
Chance B, Luo Q, Nioka S, Alsop DC, Detre JA (1997) Optical investigations of physiology: a study of intrinsic and extrinsic biomedical contrast. Philos Trans R Soc Lond B 352:707–716
Emerson GG, Segals SS (1997) Alignment of microvascular units along skeletal muscle fibers of hamster retractor. J Appl Physiol 82:42–48
Fukunaga T, Ichinose Y, Ito M, Kawakami Y, Fukashiro S (1997) Determination of fascicles length and pennation in a contracting human muscle in vivo. J Appl Physiol 82:354–358
Gray SD, Carlsswon E, Staub NC (1967) Site of increased vascular resistance during isometric muscle contraction. Am J Physiol 213:683–689
Hampson NB, Piantadoshi CA (1996) Near infrared monitoring of human skeletal muscle oxygenation during forearm ischemia. J Appl Physiol 64:2449–2457
Homma S, Eda H, Ogasawara S, Kagaya A (1996) Near-infrared estimation of O2 supply and consumption in forearm muscles working at varying intensity. J Appl Physiol 80:1279-1284
Homma S, Kagaya A (1997) Detection of oxygen consumption in different forearm muscles during handgrip exercise by spatially resolved near spectroscopy. Adv Exp Med Biol 428:327–332
Homma T, Homma S, Kagaya A (1998) Difference in oxygen supply-consumption balance in thigh muscles working together during knee-extension exercise. Jpn J Phys Fitness Sports Med 47:525–534
Kawakami Y, Abe T, Fukunaga T (1993) Muscle-fiber pennation angles are greater in hypertrophied than in normal muscles. J Appl Physiol 74:2740–2744
Kawakami Y, Ichinose Y, Fukunaga T (1998) Architectural and functional features of human triceps surae muscles during contraction. J Appl Physiol 85:398–404
Luo Q, Nioka S, Chance B (1997) Functional Near-Infrared Imager. In: Chance B, Alfano R (eds) Optical tomography and spectroscopy of tissue: theory, instrumentation, model, and human studies II. SPIE Proc 2979:84–93
Miura H, Araki H, Matoba H (1999) Relationship between oxygenation and myoelectric activity at vastus lateralis and lateral gastrocnemius muscles. Jpn J Phys Sports Med 48:413–420
Miura H, McCully K, Hong L, Nioka S, Chance B (2001) Regional difference of muscle oxygen saturation and blood volume during exercise determined by near infrared imaging device. Jpn J Physiol 51:599–606
Nilsson B, Ingvar DH (1967) Intramuscular pressure and contractile strength related to muscle blood flow in man. Scand J Clin Lab Invest Suppl 99:31–38
Otten E (1988) Concepts and models of functional architecture in skeletal muscle. In: Pandolf KB (eds) Exercise sports science review. Macmillan, New York, pp 89–137
Piiper J, Pendergast DR, Marconi C, Meyer M, Heisler N, Cerretelli P (1985) Blood flow distribution in dog gastrocnemius muscle at rest and during stimulation. J Appl Physiol 58:2068–2074
Shinohara M, Kouzaki M, Yoshihisa T, Kuno S, Fukunaga T (1999) Non-uniformity of muscle oxygenation in the quadriceps muscle during incremental knee extension. Adv Exerc Sports Physiol 5:79–87
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This study was supported in part by Grant-in-Aid for Scientific Research from the Ministry of Education & Culture of Japan (11780023) and Ono Sports Science Grant.
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Miura, H., McCully, K., Nioka, S. et al. Relationship between muscle architectural features and oxygenation status determined by near infrared device. Eur J Appl Physiol 91, 273–278 (2004). https://doi.org/10.1007/s00421-003-0964-6
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DOI: https://doi.org/10.1007/s00421-003-0964-6