Abstract
Objectives
This study was conducted to investigate the dominant sources of the urinary pyrene metabolite, 1-hydroxypyrene glucuronide (1-OHPG), in South Korean children.
Methods
Urine samples were collected from 102 non-smoking children (aged 10–14). Urinary 1-OHPG was assayed by synchronous fluorescence spectroscopy, following immuno-affinity purification using monoclonal antibody 8E11. Urinary cotinine, a metabolite of nicotine, was measured by GC/MS. Information on environmental tobacco smoke (ETS) exposure, diet, fuel type for heating home, and other possible sources of PAH exposure was collected by self-administered questionnaires.
Results
Mean (±SE) 1-OHPG levels were 1.64 (±0.06) ng/ml (range 0.04–3.27 ng/ml). Two multiple linear regression analyses (differing in how ETS was approximated: by parental smoking or urinary cotinine) revealed a positive association between urinary 1-OHPG levels and parental smoking at home (P = 0.007), log urinary cotinine (P = 0.165), frequent grilled (shell)fish consumption (P = 0.061), and living in a commercial/other zone (P = 0.007) versus a residential or industrial zone. No consistent associations were found between 1-OHPG and the child’s sex, grilled meat consumption, or fuels used to heat the home.
Conclusions
These results support that ETS, frequent grilled fish consumption, and the ambient environment are important predictors of urinary 1-OHPG levels in South Korean children.
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References
Barr DB, Wilder LC, Caudill SP et al (2005) Urinary creatinine concentrations in the U.S. population: implications for urinary biologic monitoring measurements. Environ Health Perspect 113(2):192–200
Bernert JT Jr, Turner WE, Pirkle JL et al (1997) Development and validation of sensitive method for determination of serum cotinine in smokers and nonsmokers by liquid chromatography/atmospheric pressure ionization tandem mass spectrometry. Clin Chem 43(12):2281–2291
Buchet J, Gennart J, Mercado-Calderon F et al (1992) Evaluation of exposure to polycyclic aromatic hydrocarbons in a coke production and a graphite electrode manufacturing plant: assessment of urinary excretion of 1-hydroxypyrene as a biological indicator of exposure. Br J Ind Med 49(11):761–768
Chuang J, Callahan P, Lyu C et al (1999) Polycyclic aromatic hydrocarbon exposures of children in low-income families. J Expo Anal Environ Epidemiol 9(2):85–98. doi:10.1038/sj.jea.7500003
Fiala Z, Vyskocil A, Krajak V et al (2001) Environmental exposure of small children to polycyclic aromatic hydrocarbons. Int Arch Occup Environ Health 74(6):411–420. doi:10.1007/s004200100239
Hansen A, Raaschou-Nielsen O, Knudsen L (2006) Urinary 1-hydroxypyrene in children living in city and rural residences in Denmark. Sci Total Environ 363(1–3):70–77. doi:10.1016/j.scitotenv.2005.06.017
Hu Y, Zhou Z, Xue X et al (2006) Sensitive biomarker of polycyclic aromatic hydrocarbons (PAHs): urinary 1-hydroxyprene glucuronide in relation to smoking and low ambient levels of exposure. Biomarkers 11(4):306–318. doi:10.1080/13547500600626883
IARC (1987) Overall evaluations of carcinogenicity: an updating of IARC monographs. In overall evaluations of carcinogenicity. International Agency for Research on Cancer, Lyon, France
Jongeneelen FJ (2001) Benchmark guideline for urinary 1-hydroxypyrene as biomarker of occupational exposure to polycyclic aromatic hydrocarbons. Ann Occup Hyg 45(1):3–13
Kang D, Lee K-H, Lee K-M et al (2005) Design issues in cross-sectional biomarkers studies: urinary biomarkers of PAH exposure and oxidative stress. Mutat Res 592(1–2):138–146
Kim S, Moon S, Popkin BM (2000) The nutrition transition in South Korea. Am J Clin Nutr 71(1):44–53
Lee M, Eum K, Zoh K et al (2007) 1-hydroxypyrene as a biomarker of PAH exposure among subjects living in two separate regions from a steel mill. Int Arch Occup Environ Health 80(8):671–678. doi:10.1007/s00420-007-0178-z
Mucha A, Hryhorczuk D, Serdyuk A et al (2006) Urinary 1-hydroxypyrene as a biomarker of PAH exposure in 3-year-old Ukrainian children. Environ Health Perspect 114(4):603–609
Preuss R, Roßbach B, Wilhelm M, Bruning T, Angerer J (2006) External and internal exposure to polycyclic aromatic hydrocarbons (PAH) among workers in the production of fire-proof materials—proposal of a biological monitoring guidance value. Int J Hyg Environ Health 209:575–580. doi:10.1016/j.ijheh.2006.05.005
Scherer G, Frank S, Riedel K et al (2000) Biomonitoring of exposure to polycyclic aromatic hydrocarbons of nonoccupationally exposed persons. Cancer Epidemiol Biomarkers Prev 9(4):373–380
Singh R, Tucek M, Maxa K et al (1995) A rapid and simple method for the analysis of 1-hydroxypyrene glucuronide: a potential biomarker for poly cyclic aromatic hydrocarbon exposure. Carcinogenesis 16(12):2909–2915. doi:10.1093/carcin/16.12.2909
Siwinska E, Mielzynska D, Bubak A et al (1999) The effect of coal stoves and environmental tobacco smoke on the level of urinary 1-hydroxypyrene. Mutat Res 445(2):147–153
Strickland P, Kang D (1999) Urinary 1-hydroxypyrene and other PAH metabolites as biomarkers of exposure to environmental PAH in air particulate matter. Toxicol Lett 108(2–3):191–199. doi:10.1016/S0378-4274(99)00089-2
Strickland PT, Kang D, Bowman ED et al (1994) Identification of 1-hydroxypyrene glucuronide as a major pyrene metabolite in human urine by synchronous fluorescence spectroscopy and gas chromatography-mass spectrometry. Carcinogenesis 15(3):483–487. doi:10.1093/carcin/15.3.483
Suzuki K, Yoshinaga J (2007) Inhalation and dietary exposure to polycyclic aromatic hydrocarbons and urinary 1-hydroxypyrene in non-smoking university students. Int Arch Occup Environ Health 81(1):115–121. doi:10.1007/s00420-007-0188-x
Symanski E, Bergamaschi E, Mutti A (2001) Inter- and intra-individual sources of variation in levels of urinary styrene metabolites. Int Arch Occup Environ Health 74(5):336–344. doi:10.1007/PL00007951
Thaqi A, Franke K, Merkel G et al (2005) Biomarkers of exposure to passive smoking of school children: frequency and determinants. Indoor Air 15(5):302–310. doi:10.1111/j.1600-0668.2005.00361.x
Tsai H, Wu M, Hauser R et al (2003) Exposure to environmental tobacco smoke and urinary 1-hydroxypyrene levels in preschool children. Kaohsiung J Med Sci 19(3):97–104
van Wijnen J, Slob R, Jongmans-Liedekerken G et al (1996) Exposure to polycyclic aromatic hydrocarbons among Dutch children. Environ Health Perspect 104(5):530–534. doi:10.2307/3432994
Viau C (2002) Biological monitoring of exposure to mixtures. Toxicol Lett 134(1–3):9–16. doi:10.1016/S0378-4274(02)00158-3
Viau C, As Diakit, Ruzgyt A et al (2002) Is 1-hydroxypyrene a reliable bioindicator of measured dietary polycyclic aromatic hydrocarbon under normal conditions? J Chromatogr B Analyt Technol Biomed Life Sci 778(1–2):165–177. doi:10.1016/S0378-4347(01)00465-0
Viau C, Lafontaine M, Payan JP (2004) Creatinine normalization in biological revisited: the case of 1-hydroxypyrene. Int Arch Occup Environ Health 77(3):177–185. doi:10.1007/s00420-003-0495-9
Vyskocil A, Fiala Z, Chenier V et al (2000) Assessment of multipathway exposure of small children to PAH. Environ Toxicol Pharmacol 8(2):111–118. doi:10.1016/S1382-6689(00)00032-6
Acknowledgments
This study was supported by a grant of The Korea Health 21 R&D Project, from the Ministry of Health and Welfare, (02-PJ1-PG1-CH03-0001), and by the Ministry of Environment of the Republic of Korea, as The Eco-technopia 21 Project.
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Lee, KH., Vermeulen, R., Lenters, V. et al. Determinants of urinary 1-hydroxypyrene glucuronide in South Korean children. Int Arch Occup Environ Health 82, 961–968 (2009). https://doi.org/10.1007/s00420-008-0385-2
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DOI: https://doi.org/10.1007/s00420-008-0385-2