Abstract
c-jun, a major component of AP-1 transcription factor, has a wide variety of functions. In the embryonic brain, c-jun mRNA is abundantly expressed in germinal layers around the ventricles. Although the subventricular zone (SVZ) of the adult brain is a derivative of embryonic germinal layers and contains neural precursor cells (NPCs), the c-jun expression pattern is not clear. To study the function of c-jun in adult neurogenesis, we analyzed c-jun expression in the adult SVZ by immunohistochemistry and compared it with that of the embryonic brain. We found that almost all proliferating embryonic NPCs expressed c-jun, but the number of c-jun immunopositive cells among proliferating adult NPCs was about half. In addition, c-jun was hardly expressed in post-mitotic migrating neurons in the embryonic brain, but the majority of c-jun immunopositive cells were tangentially migrating neuroblasts heading toward the olfactory bulb in the adult brain. In addition, status epilepticus is known to enhance the transient proliferation of adult NPCs, but the c-jun expression pattern was not significantly affected. These expression patterns suggest that c-jun has a pivotal role in the proliferation of embryonic NPCs, but it has also other roles in adult neurogenesis.
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Acknowledgements
We thank Professor Dr. Masayoshi Okada for his critical review. This work was supported by JSPS KAKENHI Grant-in-Aid for Scientific Research(C), Grant Number: 25430044.
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This study was supported by JSPS KAKENHI Grant Number 25430044.
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418_2016_1536_MOESM1_ESM.pptx
Online Resource 1 c-jun+ cells are not migrating cells of the embryonic brain. At E14, there is a scattering of c-jun+ cells lining the surface of the lateral ventricle that are DCX+ (arrows), and some of them are in telophase (double arrow). Single optical images of confocal microscopy are shown. Scale bar 12.5 μm. Online Resource 2 Status epilepticus enhances proliferation of adult NPCs. In the anterior part of the SVZ, a larger number of BrdU+ cells are detected in the SE group (b) than in the control group (a) 1 week after induction of SE. Light microscopy images are shown. (c) The number of BrdU+ cells is compared (n = 3). *, p<0.05. Scale bar 200 μm (PPTX 1052 KB)
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Kawashima, F., Saito, K., Kurata, H. et al. c-jun is differentially expressed in embryonic and adult neural precursor cells. Histochem Cell Biol 147, 721–731 (2017). https://doi.org/10.1007/s00418-016-1536-2
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DOI: https://doi.org/10.1007/s00418-016-1536-2