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C22-bronchial and T7-alveolar epithelial cell lines of the immortomouse are excellent murine cell culture model systems to study pulmonary peroxisome biology and metabolism

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Abstract

In pulmonary research, temperature-sensitive immortalized cell lines derived from the lung of the “immortomouse” (H-2kb-tsA58 transgenic mouse), such as C22 club cells and T7 alveolar epithelial cells type II (AECII), are frequently used cell culture models to study CC10 metabolism and surfactant synthesis. Even though peroxisomes are highly abundant in club cells and AECII and might fulfill important metabolic functions therein, these organelles have never been investigated in C22 and T7 cells. Therefore, we have characterized the peroxisomal compartment and its associated gene transcription in these cell lines. Our results show that peroxisomes are highly abundant in C22 and T7 cells, harboring a common set of enzymes, however, exhibiting specific differences in protein composition and gene expression patterns, similar to the ones observed in club cells and AECII in situ in the lung. C22 cells contain a lower number of larger peroxisomes, whereas T7 cells possess more numerous tubular peroxisomes, reflected also by higher levels of PEX11 proteins. Moreover, C22 cells harbor relatively higher amounts of catalase and antioxidative enzymes in distinct subcellular compartments, whereas T7 cells exhibit higher levels of ABCD3 and plasmalogen synthesizing enzymes as well as nuclear receptors of the PPAR family. This study suggest that the C22 and T7 cell lines of the immortomouse lung are useful models to study the regulation and metabolic function of the peroxisomal compartment and its alterations by paracrine factors in club cells and AECII.

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Acknowledgments

The authors thank Elke Richter, Andrea Textor, Bianca Pfeiffer and Karina Greve for excellent technical assistance. Furthermore, we would like to thank Profs. Denis I. Crane, Stephen J. Gould, Paul P. Van Veldhoven, Alfred Völkl and Nancy Bravermann for providing us with some antibodies (see Table 1). Our study was supported by performance-related resource allocation funds (LOM = Leistungsorientierte Mittel) of the Medical Faculty of the Justus Liebig University Giessen, Germany.

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Authors and Affiliations

  1. Institute for Anatomy and Cell Biology, Medical Cell Biology, Justus Liebig University, Aulweg 123, 35385, Giessen, Germany

    Srikanth Karnati, Saranya Palaniswamy, Mohammad Rashedul Alam, Gani Oruqaj & Eveline Baumgart-Vogt

  2. Division of Cellular Pneumology, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Germany

    Cordula Stamme

  3. Department of Anesthesiology, University of Lübeck, Lübeck, Germany

    Cordula Stamme

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  1. Srikanth Karnati
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  2. Saranya Palaniswamy
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  3. Mohammad Rashedul Alam
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  5. Cordula Stamme
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  6. Eveline Baumgart-Vogt
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Correspondence to Eveline Baumgart-Vogt.

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Karnati, S., Palaniswamy, S., Alam, M.R. et al. C22-bronchial and T7-alveolar epithelial cell lines of the immortomouse are excellent murine cell culture model systems to study pulmonary peroxisome biology and metabolism. Histochem Cell Biol 145, 287–304 (2016). https://doi.org/10.1007/s00418-015-1385-4

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