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Components of the CtBP1/BARS-dependent fission machinery

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Abstract

The brefeldin A ADP-ribosylated substrate, a member of the C-terminal-binding protein family that is referred to as CtBP1/BARS, is a dual-function protein that acts as a transcriptional co-repressor in the nucleus and as an inducer of membrane fission in the cytoplasm. In this review, we first discuss the mechanisms that enable CtBP1/BARS to shift between the nuclear transcriptional co-repressor and the cytosolic fission-inducing activities. Then, we focus on the role of CtBP1/BARS in membrane fission. CtBP1/BARS controls several fission events including macropinocytosis, fluid-phase endocytosis, COPI-coated vesicle formation, basolaterally directed post-Golgi carrier formation, and Golgi partitioning in mitosis. We report on recent advances in our understanding of the CtBP1/BARS membrane fission machineries that operate at the trans-side and at the cis-side of the Golgi complex. Specifically, we discuss how these machineries are assembled and regulated, and how they operate in the formation of the basolaterally directed post-Golgi carriers.

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Acknowledgments

We thank Dr C.P. Berrie for editorial assistance. The cited work in the authors’ laboratories was supported by the Italian Association for Cancer Research (to D.C. IG10341, to A.L. IG4700), Grant FIT DM 24/09/2009, Legge 46/82, and FaReBio, Ministry of Economy and Finance, MIUR, projects PON01-00117 and PON01-00862, and PNR-CNR Aging Program 2012-2014. C.V. was a recipient of an Italian Foundation for Cancer Research Fellowships (FIRC, Milan, Italy).

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  1. Institute of Protein Biochemistry, National Research Council, Via Pietro Castellino 111, 80131, Naples, Italy

    Carmen Valente, Alberto Luini & Daniela Corda

  2. Telethon Institute of Genetics and Medicine, Via Pietro Castellino 111, 80131, Naples, Italy

    Alberto Luini

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Correspondence to Carmen Valente.

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Valente, C., Luini, A. & Corda, D. Components of the CtBP1/BARS-dependent fission machinery. Histochem Cell Biol 140, 407–421 (2013). https://doi.org/10.1007/s00418-013-1138-1

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