Abstract
The mechanism of transport through the Golgi is still controversial, and this has led to a search for model organisms that might provide new insights. One such is the protozoan parasite, Trypanosoma brucei, which has a single Golgi whose major cargo is the GPI-anchored coat proteins that decorate the cell surface and protect the organism against immune attack through a shedding mechanism. Using published biochemical and stereological data, it is possible to show that some models for Golgi transport appear more likely than others.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Duszenko M, Ivanov IE, Ferguson MAJ, Plesken H, Cross GAM (1988) Intracellular transport of a variant surface glycoprotein in Trypanosoma brucei. J Cell Biol 106:77–86
Engstler M, Thilo L, Weise F, Grünfelder CG, Schwarz H, Boshart M, Overath P (2003) Kinetics of endocytosis and recycling of the GPI-anchored variant surface glycoprotein in Trypanosome brucei. J Cell Sci 117:1105–1115
Engstler M, Pfohl T, Herminghaus S, Boshart M, Wiegertjes G, Heddergott N, Overath P (2007) Hydrodynamic flow-mediated protein sorting on the cell surface of trypanosomes. Cell 131:505–515
Glick BS, Luini A (2011) Models for Golgi traffic: a critical assessment. Cold Spring Harb Perspect Biol. doi:10.1101/cshperspect.a005215
Grab DJ, Webster P, Verjee Y (1984) The intracellular pathway and assembly of newly-formed variable surface glycoprotein of Trypanosoma brucei. Proc Natl Acad Sci USA 81:7703–7707
Grünfelder CG, Engstler M, Weise F, Schwarz H, Stierhof Y-D, Boshart M, Overath P (2002) Accumulation of a GPI-anchored protein at the cell surface requires sorting at multiple intracellular levels. Traffic 3:547–559
Hammarton TC, Wickstead B, McKean PG (2007) Cell structure, cell division and cell cycle. In: Barry D, McCulloch R, Mottram J, Acosta-Serrano A (eds) Trypanosomes after the genome, 1st edn. Horizon Bioscience, Norfolk, pp 239–280
He YH, Ho HH, Malsam J, Chalouni C, West CM, Ullu E, Toomre D, Warren G (2004) Golgi duplication in Trypanosoma brucei. J Cell Biol 165:313–321
Jackson DG, Owen MJ, Voorheis HP (1985) A new method for the rapid purification of both membrane-bound and released forms of the variant surface glycoprotein from Trypanosoma brucei. Biochem J 230:195–202
Jin L, Pahuja KB, Wickliffe KE, Gorur A, Baumgärtel C, Schekman R, Rape M (2012) Ubiquitin-dependent regulation of COPII coat size and function. Nature 482:495–500
Mancini EJ, Clarke M, Gowen BE, Rutten T, Fuller SD (2000) Cryo-electron microscopy reveals the functional organization of an enveloped virus, Semliki Forest Virus. Mol Cell 5:255–266
Mehlert A, Bond CS, Ferguson MAJ (2002) The glycoforms of Trypanosoma brucei variant surface glycoprotein and molecular modeling of a glycosylated surface coat. Glycobiology 12:607–612
Pays E, Salmon D, Morrison LJ, Marcello L, Barry JD (2007) Antigenic variation in Trypanosoma brucei. In: Barry D, McCulloch R, Mottram J, Acosta-Serrano A (eds) Trypanosomes after the genome, 1st edn. Horizon Bioscience, Norfolk, pp 339–372
Takamori S et al (2006) Molecular anatomy of a trafficking organelle. Cell 127:831–846
Yelinek JT, He CY, Warren G (2009) Ultrastructural study of Golgi duplication in Trypanosoma brucei. Traffic 10:300–306
Acknowledgments
Thanks to Brooke Morriswood, Lars Demmel and Katy Schmidt for constructive comments.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Warren, G. Transport through the Golgi in Trypanosoma brucei . Histochem Cell Biol 140, 235–238 (2013). https://doi.org/10.1007/s00418-013-1112-y
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00418-013-1112-y