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Characterization of newly established bovine intestinal epithelial cell line

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Abstract

Membranous epithelial cells (M cells) of the follicle-associated epithelium in Peyer’s patches have a high capacity for transcytosis of several viruses and microorganisms. Here, we report that we have successfully established a bovine intestinal epithelial cell line (BIE cells) and developed an in vitro M cell model. BIE cells have a cobblestone morphology and microvilli-like structures, and strongly express cell-to-cell junctional proteins and cytokeratin, which is a specific intermediate filament protein of epithelial cells. After co-culture with murine intestinal lymphocytes or treatment with supernatant from bovine PBMC cultured with IL-2, BIE cells acquired the ability of transcytosis. Therefore, BIE cells have typical characteristics of bovine intestinal epithelial cells and also have the ability to differentiate into an M cell like linage. In addition, our results indicate that contact between immune cells and epithelial cells may not be absolutely required for the differentiation of M cells. We think that BIE cells will be useful for studying the transport mechanisms of various pathogens and also the evaluation of drug delivery via M cells.

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Acknowledgments

This research was supported by a Grant-in-Aid for Scientific Research (21380170) from the Ministry of Education, Culture, Sports, Science and Technology, BSE Control Project from the Ministry of Agriculture, Forestry and Fisheries, and Postdoctoral Fellowships for Research Abroad from Japan Society for the Promotion of Science (JSPS).

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Authors and Affiliations

  1. Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555, Japan

    Kohtaro Miyazawa, Tetsuya Hondo, Takashi Kanaya, Sachi Tanaka, Ikuro Takakura, Wataru Itani, Takahiro Yamaguchi & Hisashi Aso

  2. Section of Neuropathology, Department of Surgery, Yale Medical School, 310 Cedar St, New Haven, CT, 06510, USA

    Kohtaro Miyazawa

  3. Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Cardiganshire, SY23 3AL, UK

    Michael T. Rose

  4. Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555, Japan

    Haruki Kitazawa

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  1. Kohtaro Miyazawa
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  2. Tetsuya Hondo
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  3. Takashi Kanaya
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  4. Sachi Tanaka
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  5. Ikuro Takakura
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  6. Wataru Itani
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  7. Michael T. Rose
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  8. Haruki Kitazawa
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  9. Takahiro Yamaguchi
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  10. Hisashi Aso
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Correspondence to Hisashi Aso.

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K. Miyazawa and T. Hondo contributed equally to this work.

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Miyazawa, K., Hondo, T., Kanaya, T. et al. Characterization of newly established bovine intestinal epithelial cell line. Histochem Cell Biol 133, 125–134 (2010). https://doi.org/10.1007/s00418-009-0648-3

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