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Induction and cellular expression of tartrate resistant acid phosphatase during dextran sodium sulphate induced colitis in rats

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Abstract

The aim of this study was to investigate the cellular and molecular expression of tartrate resistant acid phosphatase (TRAP) as a marker of activated macrophages in macrophage dependent dextran sulphate sodium (DSS)-induced colitis in rats. In normal colon, TRAP+/CX3CR1+ macrophages were located in the upper part of the lamina propria. In the early stage (day 1–3) of acute colitis prior to histopathological changes, induction of the cytokines TNFα, IL-12 and IFNγ occurred concomitant with increased mRNA and enzyme activity of TRAP along with a slight increase of TRAP immunolabelling in macrophages of the upper lamina propria, suggesting induction of TRAP in resident macrophages. Among these cytokines, TNFα up-regulated TRAP expression in the RAW 264.7 monocyte/macrophage cell line. In a later phase (day 7) with fulminant colitis, a massive infiltration of macrophages including recruited TRAP+/CCR2+ cells was observed also in the lower part of the lamina propria as well as in the submuscular layer. Additionally, differentiated cellular expression of pro- and mature TRAP also suggest that mucosal macrophages in the lower part of lamina propria bordering the sub-mucosa provide a source of replenishment of macrophages situated in the upper lamina propria. In conclusion, induction of TRAP provides an early sign of macrophage responsiveness in DSS induced colitis.

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Acknowledgments

This study was supported by grants from the Swedish Research Council, Karolinska Institutet, Stockholm County Council, University of Kalmar, The Lua- ALF agreement, the Anna-Lisa and Bror Björnsson Foundation and the Magnus Bergvall foundation.

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Authors and Affiliations

  1. Division of Pathology F46, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, 141 86, Stockholm, Sweden

    Pernilla Lång & Göran Andersson

  2. Department of Clinical Bacteriology, Sahlgrenska University Hospital, Göteborg, Sweden

    Stefan Lange

  3. Department of Chemistry and Biomedical Sciences, University of Karlstad, Karlstad, Sweden

    Dick Delbro

  4. Department of Surgery, Sahlgrenska University Hospital, Göteborg, Sweden

    Dick Delbro

  5. School of Pure and Natural Sciences, University of Kalmar, Kalmar, Sweden

    Dick Delbro

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  1. Pernilla Lång
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Correspondence to Göran Andersson.

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Lång, P., Lange, S., Delbro, D. et al. Induction and cellular expression of tartrate resistant acid phosphatase during dextran sodium sulphate induced colitis in rats. Histochem Cell Biol 132, 599–612 (2009). https://doi.org/10.1007/s00418-009-0647-4

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