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Expression pattern of serine protease inhibitor kazal type 3 (Spink3) during mouse embryonic development

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Abstract

Recent evidence shows that the serine protease inhibitor Kazal type 3 (Spink3) has more diverse functions than expected. To gain insight into its function, we analyzed the spatiotemporal expression profile of Spink3, using in situ hybridization (ISH) and a Spink3 +/lacZ knock-in mouse, in which lacZ was inserted into the Spink3 locus. Spink3 lacZ expression was first observed in the foregut, midgut, hindgut and the forebrain/midbrain junction region at 9.5 days post coitus (dpc). In the pancreas, Spink3 mRNA was detected at 11.5 dpc, before formation of the typical shape of the exocrine structure of the pancreas. Acinar cell expression was clearly identified by 13.5 dpc. After differentiation of the intestinal tract, Spink3 lacZ expression was observed in the large intestine at 11.5 dpc, followed by expression in the small intestine at 13.5 dpc, before appearance of intestinal digestive enzymes. Spink3 mRNA and Spink3 lacZ activity were also detected in other tissues, including the mesonephric tubules and the urogenital ridge at 11.5 dpc, the genital swelling at 13.5 dpc, the ductus epididymis at 17.5 dpc, and the seminal vesicle at 8 weeks. These data suggest that Spink3 may play important roles in proliferation and/or differentiation of various cell types during development.

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Abbreviations

Spink3:

Serine protease inhibitor Kazal type 1

PSTI:

Pancreatic secretory trypsin inhibitor

EGF:

Epidermal growth factor

X-gal:

4-Chloro-5-bromo-3-indolyl-β-D-galactopyranoside

CCK:

Cholecystokinin

ES:

Embryonic stem

ISH:

In situ hybridization

dpc:

Days post coitum

dpp:

Days post partum

DIG:

Digoxigenin

RE:

Right element mutant lox site

TATI:

Tumor associated trypsin inhibitor

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Acknowledgments

We sincerely appreciate Mrs. Michiyo Nakata for her excellent work on tissue section preparation. We would like also to show our thanks to Mr. Ryo Yamashita for his valuable advice on the X-gal staining technique. This work was supported, in part, by KAKENHI (Grant-in-Aid for Scientific Research) in Priority Areas “Integrative Research Toward the Conquest of Cancer” and a Grant-in-Aid for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, a grant from the Osaka Foundation for the Promotion of Clinical Immunology and a grant from the Pancreas Research Foundation of Japan.

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Authors and Affiliations

  1. Division of Developmental Genetics, Institute of Molecular Embryology and Genetics, Kumamoto University, Honjo 2-2-1, 860-0811, Kumamoto, Japan

    Jun Wang, Masaki Ohmuraya, Gang Zhao, Kimi Araki & Ken-ichi Yamamura

  2. Department of Surgery, Faculty of Medical and Pharmaceutical Science, Kumamoto University, 1-1-1 Honjo, Kumamoto, 860-8556, Japan

    Masaki Ohmuraya, Masahiko Hirota & Hideo Baba

  3. Division of Cell Pathology, Faculty of Medical and Pharmaceutical Science, Kumamoto University, 1-1-1 Honjo, Kumamoto, 860-8556, Japan

    Motohiro Takeya

Authors
  1. Jun Wang
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  2. Masaki Ohmuraya
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  3. Masahiko Hirota
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  4. Hideo Baba
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  5. Gang Zhao
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  6. Motohiro Takeya
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  7. Kimi Araki
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  8. Ken-ichi Yamamura
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Corresponding author

Correspondence to Ken-ichi Yamamura.

Additional information

This work was supported, in part, by KAKENHI (Grant-in-Aid for Scientific Research) in Priority Areas “Integrative Research Toward the Conquest of Cancer” and by a Grant-in-Aid for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, a grant from the Osaka Foundation for the Promotion of Clinical Immunology and a grant from the Pancreas Research Foundation of Japan.

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Wang, J., Ohmuraya, M., Hirota, M. et al. Expression pattern of serine protease inhibitor kazal type 3 (Spink3) during mouse embryonic development. Histochem Cell Biol 130, 387–397 (2008). https://doi.org/10.1007/s00418-008-0425-8

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