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Type I diabetic bone phenotype is location but not gender dependent

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Abstract

Bone is highly dynamic and responsive. Bone location, bone type and gender can influence bone responses (positive, negative or none) and magnitude. Type I diabetes induces bone loss and increased marrow adiposity in the tibia. We tested if this response exhibits gender and location dependency by examining femur, vertebrae and calvaria of male and female, control and diabetic BALB/c mice. Non-diabetic male mice exhibited larger body, muscle, and fat mass, and increased femur BMD compared to female mice, while vertebrae and calvarial bone parameters did not exhibit gender differences. Streptozotocin-induced diabetes caused a reduction in BMD at all sites examined irrespective of gender. Increased marrow adiposity was evident in diabetic femurs and calvaria (endochondrial and intramembranous formed bones, respectively), but not in vertebrae. Leptin-deficient mice also exhibit location dependent bone responses and we found that serum leptin levels were significantly lower in diabetic compared to control mice. However, in contrast to leptin-deficient mice, the vertebrae of T1-diabetic mice exhibit bone loss, not gain. Taken together, our findings indicate that TI-diabetic bone loss in mice is not gender, bone location or bone type dependent, while increased marrow adiposity is location dependent.

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Acknowledgments

The authors would like to thank the Investigative Histology Laboratory in the Department of Physiology, Division of Human Pathology at Michigan State University for assistance with the histological studies, and Regina Irwin, Sergiu Botolin and Katie Motyl for their technical expertise and assistance with diabetic mice and analyses, and Gavin Gibson and Laura Harris for their critical review of this manuscript. This work was funded by a grant from NIH (DK061184) to LRM.

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  1. Department of Physiology, Biomedical Imaging Research Center, Michigan State University, 2201 Biomedical Physical Science Bldg, East Lansing, MI, 48824, USA

    Lindsay M. Martin & Laura R. McCabe

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Correspondence to Laura R. McCabe.

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Martin, L.M., McCabe, L.R. Type I diabetic bone phenotype is location but not gender dependent. Histochem Cell Biol 128, 125–133 (2007). https://doi.org/10.1007/s00418-007-0308-4

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