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Secreted modular calcium-binding protein-1 localization during mouse embryogenesis

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Abstract

BM-40 is an extracellular matrix-associated protein and is characterized by an extracellular calcium-binding domain as well as a follistatin-like domain. Secreted modular calcium-binding protein-1 (SMOC-1) is a new member of the BM-40 family. It consists of two thyroglobulin-like domains, a follistatin-like domain and a new domain without known homologues and is expressed ubiquitously in many adult murine tissues. Immunofluorescence studies, as well as immunogold electron microscopy, have confirmed the localization of SMOC-1 in or around basement membranes of adult murine skin, blood vessels, brain, kidney, skeletal muscle, and the zona pellucida surrounding the oocyte. In the present work, light microscopic immunohistochemistry has revealed that SMOC-1 is localized in the early mouse embryo day 7 throughout the entire endodermal basement membrane zone of the embryo proper. SMOC-1 mRNA is synthesized, even in early stages of mouse development, by mesenchymal as well as epithelial cells deriving from all three germ layers. In embryonic stage day 12, and fetal stages day 14, 16, and 18, the protein is present in the basement membrane zones of brain, blood vessels, skin, skeletal muscle, lung, heart, liver, pancreas, intestine, and kidney. This broad and organ-specific distribution suggests multifunctional roles of SMOC-1 during mouse embryogenesis.

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Acknowledgments

We would like to thank Mats Paulsson, Institute for Biochemistry, University of Cologne, for the antibodies. We would also like to thank Christina Zelent for technical assistance and Cyrilla Maelicke B.Sc. for editing the manuscript. Parts of the work were taken from the doctoral thesis of Antje Schall.

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Authors and Affiliations

  1. Department of Prosthodontics, Georg-August-Universität Göttingen, 37075, Göttingen, Germany

    Nikolaus Gersdorff & Matthias Müller

  2. Department of Histology, Georg-August-Universität Göttingen, 37075, Göttingen, Germany

    Antje Schall & Nicolai Miosge

Authors
  1. Nikolaus Gersdorff
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  2. Matthias Müller
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  3. Antje Schall
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  4. Nicolai Miosge
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Correspondence to Nicolai Miosge.

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Gersdorff, N., Müller, M., Schall, A. et al. Secreted modular calcium-binding protein-1 localization during mouse embryogenesis. Histochem Cell Biol 126, 705–712 (2006). https://doi.org/10.1007/s00418-006-0200-7

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  • DOI: https://doi.org/10.1007/s00418-006-0200-7

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