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Toward functional glycomics by localization of tissue lectins: immunohistochemical galectin fingerprinting during diethylstilbestrol-induced kidney tumorigenesis in male Syrian hamster

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Abstract

The current study focused on galectins (-1, -3, -4, -7, and –8) and deliberately performed immunohistochemical fingerprinting to explore their complexity in a context of experimental renal carcinogenesis. The diethylstilbestrol (DES)-induced renal tumors in male Syrian hamster kidney (SHKT) represent a unique animal model for the study of estrogen-dependent renal malignancies. Kidney sections of DES-treated hamsters (3 days to 11 months of DES exposure) were analyzed by immunohistochemistry using a panel of non-crossreactive antibodies raised against galectins-1, -3, -4, -7, and -8. Levels of expression were quantitatively determined by using computer-assisted microscopy on immunostained tissue sections. Except for galectin-4, all above mentioned galectins were expressed in kidney tumors. Small clusters of galectin-1-positive, most likely preneoplastic cells at the corticomedullary junction were already evident 1 week after DES administration. Galectin-1 and -3 expression was apparently associated with the first steps of the neoplastic transformation, because small tumorous buds were found to be positive after 1 month of treatment. In contrast, galectins-7 and -8 were detected in large tumors and medium-sized tumors, respectively, thereby indicating an involvement in later stages of DES-induced SHKT. Galectins-1, -3, -7, and -8 were also detected by immunofluorescence staining in the HKT-1097 cell line established from SHKT, thus illustrating the stability of galectin expression in tumor cells. Our data document the presence and differential regulation of galectins in the course of renal tumorigenesis in the model of DES-induced SHKT.

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Acknowledgements

R. Kiss and G. Laurent are Research Director and Senior Research Associate, respectively, of the National Fund for Scientific Research (Belgium). G. Laurent is the recipient of a grant (number 3.4512.03) from the Belgian Fund for Medical Scientific Research. The expert technical assistance of G. Ninfa is gratefully acknowledged.

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Authors and Affiliations

  1. Laboratory of Histology, Faculty of Medicine and Pharmacy, University of Mons-Hainaut, Avenue du Champ de Mars, 6 - Pentagone 1B, 7000, Mons, Belgium

    Sven Saussez, Denis Nonclercq, Guy Laurent & Gérard Toubeau

  2. Department of Anatomy, Faculty of Medicine and Pharmacy, University of Mons-Hainaut, Avenue du champ de Mars, 6 - Pentagone 1B, 7000, Mons, Belgium

    Sven Saussez

  3. Laboratory of Biological Chemistry, University of Mons-Hainaut, Avenue du champ de Mars, 6 - Pentagone 3B, 7000, Mons, Belgium

    Rudy Wattiez

  4. Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Veterinärstrasse 13, 80539, Munich, Germany

    Sabine André, Herbert Kaltner & Hans-Joachim Gabius

  5. Laboratory of Toxicology, Institute of Pharmacy, Université Libre de Bruxelles, Campus Plaine CP 205/1, Blvd du Triomphe, 1050, Bruxelles, Belgium

    Robert Kiss

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  1. Sven Saussez
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Correspondence to Sven Saussez.

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Saussez, S., Nonclercq, D., Laurent, G. et al. Toward functional glycomics by localization of tissue lectins: immunohistochemical galectin fingerprinting during diethylstilbestrol-induced kidney tumorigenesis in male Syrian hamster. Histochem Cell Biol 123, 29–41 (2005). https://doi.org/10.1007/s00418-004-0733-6

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