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Blood-brain barrier disruption in the hypothalamus of young adult spontaneously hypertensive rats

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Abstract

Vascular permeability and endothelial glycocalyx were examined in young adult spontaneously hypertensive rats (SHR), stroke-prone SHR (SHRSP), and Wistar Kyoto rats (WKY) as a control, in order to determine earlier changes in the blood-brain barrier (BBB) in the hypothalamus in chronic hypertension. These rats were injected with horseradish peroxidase (HRP) as an indicator of vascular permeability. Brain slices were developed with a chromogen and further examined with cationized ferritin, a marker for evaluating glycocalyx. Staining for HRP was seen around vessels in the hypothalamus of SHR and SHRSP, but was scarce in WKY. The reaction product of HRP appeared in the abluminal pits of endothelial cells and within the basal lamina of arterioles, showing increased vascular permeability in the hypothalamus of SHR and SHRSP, whereas there were no leaky vessels in the frontal cortex of SHR and SHRSP, or in both areas of WKY. The number of cationized ferritin particles binding to the capillary endothelial cells was decreased in the hypothalamus of SHR and SHRSP, while the number decreased in the frontal cortex of SHRSP, compared with those in WKY. Cationized ferritin binding was preserved in some leaky arterioles, while it was scarce or disappeared in other leaky vessels. These findings suggest that BBB disruption occurs in the hypothalamus of 3-month-old SHR and SHRSP, and that endothelial glycocalyx is markedly damaged there without a close relationship to the early changes in the BBB.

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Acknowledgements

This research was supported by a Budget from the Ministry of Education, Culture, Sports, Science and Technology, Japan. The authors thank Ms C. Ishikawa and Ms Y. Fujiwara for technical and editorial assistance.

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Authors and Affiliations

  1. Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, 761-0793, Kagawa, Japan

    Masaki Ueno, Haruhiko Sakamoto, Ying-Jun Liao & Masayuki Onodera

  2. Department of Surgery, Faculty of Medicine, Kagawa University, Kagawa, Japan

    Cheng-Long Huang

  3. Life Science Research Center, Kagawa University, Kagawa, Japan

    Hiroshi Miyanaka & Toshitaka Nakagawa

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  1. Masaki Ueno
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  2. Haruhiko Sakamoto
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  3. Ying-Jun Liao
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  4. Masayuki Onodera
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  5. Cheng-Long Huang
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  6. Hiroshi Miyanaka
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  7. Toshitaka Nakagawa
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Correspondence to Masaki Ueno.

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Ueno, M., Sakamoto, H., Liao, YJ. et al. Blood-brain barrier disruption in the hypothalamus of young adult spontaneously hypertensive rats. Histochem Cell Biol 122, 131–137 (2004). https://doi.org/10.1007/s00418-004-0684-y

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