Skip to main content

Advertisement

SpringerLink
Log in

Immunolocalization of vascular endothelial growth factor in rat condylar cartilage during postnatal development

  • Original Paper
  • Published:
Histochemistry and Cell Biology Aims and scope Submit manuscript

Abstract

It is well known that angiogenesis is essential for the replacement of cartilage by bone during skeletal growth and regeneration. To address angiogenesis of endochondral ossification in the condyle, we examined the appearance of vascular endothelial growth factor (VEGF) and its receptor Flt-1 in condylar cartilage of the growing rat. The early expression of VEGF at various sites during condylar cartilage development indicates that VEGF plays a role in the regulation of angiogenesis at each site of bone formation. From the findings of Flt-1 immunoreactivity, the VEGF produced by the chondrocytes of the hypertrophic zone should contribute to the promotion of endothelial cell proliferation and to stimulate migration and activation of osteoclasts in condylar cartilage, resulting in the invasion of these cells into the mineralized zone.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3a–c
Fig. 4a–d
Fig. 5a–c

Similar content being viewed by others

References

  • Baleon B, Sozzani S, Zhou D, Weich HA, Mantovani A, Marme D (1996) Migration of human monocytes in response to vascular endothelial growth factor (VEGF) is mediated via the VEGF receptor flt-1. Blood 87:3336–3343

    PubMed  Google Scholar 

  • Carlevaro MF, Cermelli S, Cancedda R, Cancedda DF (2000) Vascular endothelial growth factor (VEGF) in cartilage neovascularization and chondrocyte differentiation: auto-paracrine role during endochondral bone formation. J Cell Sci 113:59–69

    CAS  PubMed  Google Scholar 

  • Christofori G, Naik P, Hanahan D (1995) Vascular endothelial growth factor and its receptors, flt-1, are expressed in normal pancreatic islets and throughout islet cell tumorigenesis. Mol Endocrinol 9:1760–1770

    Article  CAS  PubMed  Google Scholar 

  • Claffey KP, Wilkison WO, Spiegelman BM (1992) Vascular endothelial growth factor. Regulation by cell differentiation and activated second messenger pathways. J Biol Chem 267:16317–16322

    CAS  PubMed  Google Scholar 

  • Conn G, Bayne ML, Soderman DD, Kwok PW, Sullivan KA, Palisi TM, Hope DA, Thomas KA (1990) Amino acid and cDNA sequences of a vascular endothelial cell mitogen that is homologous to platelet-derived growth factor. Proc Natl Acad Sci U S A 87:2628–2632

    CAS  PubMed  Google Scholar 

  • Connolly DT (1991) Vascular permeability factor: a unique regulator of blood vessel function. J Cell Biochem 47:219–223

    CAS  PubMed  Google Scholar 

  • Copray JC, Jansen HW, Duterloo HS (1986) Growth and growth pressure of mandibular condylar and some primary cartilages of the rat in vitro. Am J Orthod Dentofacial Orthop 90:19–28

    CAS  PubMed  Google Scholar 

  • Dibbets JM, Carlson D (1995) Implications of temporomandibular disorders for facial growth and orthodontic treatment. Semin Orthod 1:258–272

    CAS  PubMed  Google Scholar 

  • Ferrara N, Davis-Smyth T (1997) The biology of vascular endothelial growth factor. Endocr Rev 18:4–25

    Article  CAS  PubMed  Google Scholar 

  • Garcia-Ramirez M, Toran N, Andaluz P, Carrascosa A, Audi L (2000) Vascular endothelial growth factor is expressed in human fetal growth cartilage. J Bone Miner Res 15:534–540

    CAS  PubMed  Google Scholar 

  • Gerber HP, Vu TH, Ryan AM, Kowalski J, Werb Z, Ferrara N (1999) VEGF couples hypertrophic cartilage remodelling, ossification and angiogenesis during endochondral ossification. Nat Med 5:623–628

    Article  CAS  PubMed  Google Scholar 

  • Harada S, Nagy JA, Sullivan KA, Thomas KA, Endo N, Rodan GA, Rodan SB (1994) Induction of vascular endothelial growth factor expression by prostaglandin E2 and E1 in osteoblasts. J Clin Invest 93:2490–2496

    CAS  PubMed  Google Scholar 

  • Harper J, Klagsbrun M (1999) Cartilage to bone: angiogenesis leads the way. Nat Med 5:617–618

    Article  CAS  PubMed  Google Scholar 

  • Horner A, Bishop NJ, Bord S, Beeton C, Kelsall AW, Coleman N, Compston JE (1999) Immunolocalisation of vascular endothelial growth factor (VEGF) in human neonatal growth plate cartilage. J Anat 194:519–524

    Article  CAS  PubMed  Google Scholar 

  • Horner A, Bord S, Kelsall AW, Coleman N, Compston JE (2001) Tie2 ligands angiopoietin-1 and angiopoietin-2 are coexpressed with vascular endothelial cell growth factor in growing human bone. Bone 28:65–71

    Article  CAS  PubMed  Google Scholar 

  • Ichigatani M, Saga T, Yamaki K, Yoshizuka M (2001) Appearance of vascular endothelial growth factor (VEGF) in femoral head in the growing rat. Histol Histopathol 16:463–468

    CAS  PubMed  Google Scholar 

  • Kearney JB, Kappas NC, Ellerstrom C, DiPaola FW, Bautch VL(2004) The VEGF receptor flt-1 (VEGFR-1) is a positive modulator of vascular sprout formation and branching morphogenesis. Blood. [Epub ahead of print]

  • Leung DW, Cachianes G, Kuang WJ, Goeddel DV, Ferrara N (1989) Vascular endothelial growth factor is a secreted angiogenic mitogen. Science 246:1306–1309

    CAS  PubMed  Google Scholar 

  • Nakagawa M, Kaneda T, Arakawa T, Morita S, Sato T, Yamada T, Hanada K, Kumegawa M, Hakeda Y (2000) Vascular endothelial growth factor (VEGF) directly enhances osteoclastic bone resorption and survival of mature osteoclasts. FEBS Lett 473:161–164

    Article  CAS  PubMed  Google Scholar 

  • Niida S, Kaku M, Amano H, Yoshida H, Kataoka H, Nishikawa S, Tanne K, Maeda N, Nishikawa S, Kodama H (1999) Vascular endothelial growth factor can substitute for macrophage colony-stimulating factor in the support of osteoclastic bone resorption. J Exp Med 190:293–298

    Article  CAS  PubMed  Google Scholar 

  • Pepper MS, Ferrara N, Orci L, Montesano R (1991) Vascular endothelial growth factor (VEGF) induces plasminogen activators and plasminogen activator inhibitor-1 in microvascular endothelial cells. Biochem Biophys Res Commun 181:902–906

    CAS  PubMed  Google Scholar 

  • Phillips HS, Hains J, Leung DW, Ferrara N (1990) Vascular endothelial growth factor is expressed in rat corpus luteum. Endocrinology 127:965–967

    CAS  PubMed  Google Scholar 

  • Pufe T, Petersen W, Tillmann B, Mentlein R (2001a) The splice variants VEGF121 and VEGF189 of the angiogenic peptide vascular endothelial growth factor are expressed in osteoarthritic cartilage. Arthritis Rheum 44:1082–1088

    Article  CAS  PubMed  Google Scholar 

  • Pufe T, Petersen W, Tillmann B, Mentlein R (2001b) Splice variants VEGF121 and VEGF165 of the angiogenic peptide vascular endothelial cell growth factor are expressed in the synovial tissue of patients with rheumatoid arthritis. J Rheumatol 28:1482–1485

    CAS  PubMed  Google Scholar 

  • Simon M, Grone HJ, Johren O, Kullmer J, Plate KH, Risau W, Fuchs E (1995) Expression of vascular endothelial growth factor and its receptors in human renal ontogenesis and in adult kidney. Am J Physiol 268:F240–F250

    CAS  PubMed  Google Scholar 

  • Takano-Yamamoto T, Soma S, Nakagawa K, Kobayashi Y, Kawakami M, Sakuda M (1991) Comparison of the effects of hydrostatic compressive force on glycosaminoglycan synthesis and proliferation in rabbit chondrocytes from mandibular condylar cartilage, nasal septum, and spheno-occipital synchondrosis in vitro. Am J Orthod Dentofacial Orthop 99:448–455

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This research was supported by a grant (number 14571950) for Science Research from the Ministry of Education, Science and Culture, Japan.

Author information

Authors and Affiliations

  1. Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-ku, 734-8553, Hiroshima, Japan

    Junko Aoyama, Eiji Tanaka, Koichi Hanaoka, Yukiko Hattori, Akiko Sasaki, Mineo Watanabe & Kazuo Tanne

  2. Department of Oral Maxillofacial Pathobiology, Hiroshima University Graduate School of Biomedical Sciences, Japan

    Mutsumi Miyauchi & Takashi Takata

Authors
  1. Junko Aoyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eiji Tanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mutsumi Miyauchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Takashi Takata
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Koichi Hanaoka
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yukiko Hattori
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Akiko Sasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Mineo Watanabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Kazuo Tanne
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Eiji Tanaka.

Additional information

Junko Aoyama and Eiji Tanaka contributed equally to this work

Rights and permissions

Reprints and permissions

About this article

Cite this article

Aoyama, J., Tanaka, E., Miyauchi, M. et al. Immunolocalization of vascular endothelial growth factor in rat condylar cartilage during postnatal development. Histochem Cell Biol 122, 35–40 (2004). https://doi.org/10.1007/s00418-004-0671-3

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00418-004-0671-3

Keywords

Search

Navigation