Abstract
The study of function and regulation of the phenotype of alveolar type I (AT I) epithelial cells is limited by the rareness of suitable cell lines or primary cultures of this cell type. We describe in the present study the type I-like rat epithelial cell line R3/1. This cell line displays in vitro a phenotype with several characteristic features of AT I cells. R3/1 cells were analysed for mRNA and protein content of markers related to the AT I cell type (T1α, ICAM-1, connexin-43, caveolins-1 and -2) and AT II phenotypes [surfactant proteins (SPs) A, B, C and D]. The mRNAs for SPs were found to be at a low level. Moderate protein levels for SP-A and SP-B were found, and SP-C and SP-D proteins were not detectable. R3/1 cells are positive for CD44s, E-cadherin, cytokeratin, vimentin and RAGE, and bind the lectins BPA and SBA. For demonstration of the suitability of R3/1 cells for in vitro studies on epithelial injury, the cells were treated with bleomycin. As shown by real-time RT-PCR and immunoblotting, bleomycin-treatment of R3/1 cells resulted in a decrease in mRNA and protein for both caveolin-1 and caveolin-2 in comparison with controls. The AT I-like cell line R3/1 may serve as a promising tool for the study of lung cell biology.
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Acknowledgements
The authors thank Dr. M.C. Williams (Boston) for helpful discussion prior to the submission of the manuscript. The authors wish to thank P. Peche, B. Rost, K. Pehlke, S. Bramke and D. Streichert for their excellent technical assistance and E. Kuhlisch, Institute of Medical Informatics and Biometrics, for performing the statistical analysis. The technical support of T. Schwalm for preparation of figures is acknowledged with gratitude.
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Roland Koslowski and Kathrin Barth contributed equally to the study
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Koslowski, R., Barth, K., Augstein, A. et al. A new rat type I-like alveolar epithelial cell line R3/1: bleomycin effects on caveolin expression. Histochem Cell Biol 121, 509–519 (2004). https://doi.org/10.1007/s00418-004-0662-4
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DOI: https://doi.org/10.1007/s00418-004-0662-4