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IL1-β and TNF-α induce changes in the nuclear polyphosphoinositide signalling system in osteoblasts similar to that occurring in patients with rheumatoid arthritis: an immunochemical and immunocytochemical study

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Abstract

Osteoarthritis (OA) and rheumatoid arthritis (RA) are common joint diseases that can lead to destruction of cartilage and structural changes in the subchondral bone. In this study we show by western blot and quantitative immunocytochemistry that nuclear phospholipase C β1 (PLCβ1) and phosphatidylinositol 4,5-bisphosphate (PIP2), two key elements of the polyphosphoinositide signal transduction system that regulate different cellular processes, increase in primary osteoblast cultures of RA patients when compared with post-traumatic after fall (PT) patients, whilst those of OA are not significantly affected. Moreover, we demonstrate that these alterations could be induced in PT osteoblasts by proinflammatory cytokines IL-1β and TNF-α. This suggests that proinflammatory cytokines, highly produced by RA infiltrating mononuclear cells, can modulate the nuclear polyphosphoinositide signalling pathway of the osteoblasts involved in bone remodelling.

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Fig. 1a–e.
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Fig. 3a–g.

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Acknowledgements

The authors thank Mr. A. Valmori for technical assistance. This work was supported by grants from C.N.R., University of Bologna "Funds for selected research topics", MURST Cofin 2001 and I.O.R. Ricerca Corrente.

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Authors and Affiliations

  1. Istituto di Neuroscienze, CNR, via Moruzzi 1, 56124, Pisa, Italy

    Carmela Trimarchi & Nadir Mario Maraldi

  2. Sezioni di Bologna c/o IOR, ITOI-CNR, via di Barbiano 1/10, 40136, Bologna, Italy

    Nicoletta Zini

  3. Laboratorio di Immunologia e Genetica, IOR, via di Barbiano 1/10, 40136, Bologna, Italy

    Gina Lisignoli, Francesco Grassi & Andrea Facchini

  4. Istituto di Istologia ed Embriologia Generale, Università di Bologna, via Belmeloro 8, 40126, Bologna, Italy

    Liliana Solimando & Lia Guidotti

  5. Laboratorio di Biologia Cellulare e Microscopia Elettronica, IOR, via di Barbiano 1/10, 40136, Bologna, Italy

    Alberto Bavelloni

  6. Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell'Apparato Locomotore, Università di Bologna, Bologna, Italy

    Nadir Mario Maraldi

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  1. Nicoletta Zini
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  2. Gina Lisignoli
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  7. Carmela Trimarchi
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  8. Andrea Facchini
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  9. Nadir Mario Maraldi
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Correspondence to Nadir Mario Maraldi.

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Zini, N., Lisignoli, G., Solimando, L. et al. IL1-β and TNF-α induce changes in the nuclear polyphosphoinositide signalling system in osteoblasts similar to that occurring in patients with rheumatoid arthritis: an immunochemical and immunocytochemical study. Histochem Cell Biol 120, 243–250 (2003). https://doi.org/10.1007/s00418-003-0563-y

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  • DOI: https://doi.org/10.1007/s00418-003-0563-y

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