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Localization of brain-type fatty acid-binding protein in Kupffer cells of mice and its transient decrease in response to lipopolysaccharide

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Abstract

Brain-type fatty acid-binding protein (B-FABP) was localized in Kupffer cells of liver of postnatal day 10 (P10) and older mice in immunolight and electron microscopy as well as by in situ hybridization histochemistry. The immunoreaction products were localized in the cytoplasmic matrix but not within the nucleus. After peritoneal injection of lipopolysaccharide (LPS), the immunoreaction for B-FABP decreased markedly in Kupffer cells at 1 h postinjection and thereafter gradually recovered to the preinjection level by 24 h postinjection, although no decrease in the mRNA expression was detected in Northern blotting throughout the course after the injection. The specific localization of B-FABP, but not the other FABPs, in Kupffer cells, and its rapid decrease after LPS injection suggest the intimate involvement of B-FABP in Kupffer cells in the inflammatory reaction, probably through mediation of n-3 polyunsaturated fatty acids, which are strong binders of B-FABP.

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Acknowledgements

This work was supported by grants from the Ministry of Education, Science, and Culture of Japan, numbers 14370002 and 14657623 (to H.K.) and number 14580720 (to Y.O.).

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Authors and Affiliations

  1. Division of Histology, Department of Cell Biology, Graduate School of Medical Science, Tohoku University, 980-8575, Sendai, Japan

    Soha Abdelkawi Abdelwahab, Yuji Owada, Noriko Kitanaka, Hiroo Iwasa, Hiroyuki Sakagami & Hisatake Kondo

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  1. Soha Abdelkawi Abdelwahab
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  2. Yuji Owada
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  3. Noriko Kitanaka
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  4. Hiroo Iwasa
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  5. Hiroyuki Sakagami
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  6. Hisatake Kondo
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Correspondence to Yuji Owada.

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Abdelwahab, S.A., Owada, Y., Kitanaka, N. et al. Localization of brain-type fatty acid-binding protein in Kupffer cells of mice and its transient decrease in response to lipopolysaccharide. Histochem Cell Biol 119, 469–475 (2003). https://doi.org/10.1007/s00418-003-0538-z

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  • DOI: https://doi.org/10.1007/s00418-003-0538-z

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