Abstract
Purpose
The aim of this study was to explore the pathological changes of the nasolacrimal duct in rabbits with experimentally induced obstructive dacryocystitis in correlation with lacrimal endoscopic findings.
Methods
The rabbit model of obstructive dacryocystitis was created by injecting 0.15 ml of self-curing resin into the lacrimal duct. The control group received 0.15 ml of normal saline. Within 16 weeks after the obstructive, lacrimal endoscopy and pathological examination of the nasolacrimal duct were conducted at different time points of 1, 2, 4, 8, and 16 weeks.
Results
In the control group, lacrimal endoscopy revealed pink and smooth mucosa; and the pathological analysis revealed an epithelial layer that was composed of superficial columnar cells and a deep basal epithelial layer. The experimental rabbits showed clinical manifestations of obstructive dacryocystitis a week after the injection of self-curing resin. At weeks 1 and 2, the lacrimal endoscopy showed mucosal hyperemia and hemorrhagic spots on the nasolacrimal duct; and the pathological features included epithelial cell swelling and inflammatory cell infiltration. At weeks 4 and 8, the experimental group showed alternatively red and white mucosa under the lacrimal endoscopy, and the pathological features included proliferative epithelium accompanied by papillary hyperplasia. At week 16, the experimental group showed pale and coarse mucosa and white membrane-like layer covering the mucosal surface, and the pathological features included epithelial necrosis, squamous metaplasia, and sub-epithelial fibrosis.
Conclusion
The mucosa of the nasolacrimal duct showed different pathological features at different time points after lacrimal duct obstruction, which was well correlated with the endoscopic findings. It is possible to predict the pathological stages by the endoscopic observation in NLOD patients.
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References
Lorena SH, Silva JA, Scarpi MJ (2013) Congenital nasolacrimal duct obstruction in premature children. J Pediatr Ophthalmol Strabismus 50(4):239–244
Woog JJ (2007) The incidence of symptomatic acquired lacrimal outflow obstruction among residents of Olmsted County, Minnesota, 1976-2000 (an American Ophthalmological Society thesis). Trans Am Ophthalmol Soc 105:649–666
Ali MJ (2015) Pediatric acute dacryocystitis. Ophthal Plast Reconstr Surg 31(5):341–347
Coumou AD, Genders SW, Smid TM et al (2017) Endoscopic dacryocystorhinostomy: long-term experience and outcomes. Acta Ophthalmol 95(1):74–78
Mimura M, Ueki M, Oku H et al (2016) Effect of rebamipide ophthalmic suspension on the success of lacrimal stent intubation. Graefes Arch Clin Exp Ophthalmol 254(2):385–389
Baek JS, Lee S, Lee JH et al (2016) Predictors of silicone tube intubation success in patients with lacrimal drainage system stenosis. Korean J Ophthalmol 30(3):157–162
Koturovic Z, Knezevic M, Rasic DM (2017) Clinical significance of routine lacrimal sac biopsy during dacryocystorhinostomy: a comprehensive review of literature. Bosn J Basic Med Sci 17(1):1–8
Paulsen FP, Thale AB, Maune S et al (2001) New insights into the pathophysiology of primary acquired dacryostenosis. Ophthalmology 108(12):2329–2336
Xiang N, Liu R, Zhang SJ et al (2016) A comparative study of lacrimal magnetic resonance hydrography and lacrimal endoscopy examination in the diagnosis and treatment of lacrimal duct obstructive diseases. Zhonghua Yan Ke Za Zhi 52(2):117–122
Emmerich KH, Emmerich GM, Steinkogler FJ et al (2010) How did lacrimal endoscopy influence lacrimal surgery? Klin Monatsbl Augenheilkd 227(7):559–563
Hou K, Ai T, Liu R et al (2017) Modeling chronic Dacryocystitis in rabbits by nasolacrimal duct obstruction with self-curing resin. J Ophthalmol. https://doi.org/10.1155/2017/3438041
Huang YK, Wang Z, Chen W et al (2009) Observation on treatment of lacrimal passage obstruction under lacrimal endoscope. Zhonghua Yan Ke Za Zhi 45(6):498–502
Emmerich KH, Rusenberg HW, Amin S (2014) Modern, minimally invasive surgery of the lacrimal duct system. Ophthalmologe 111(9):887–895
Perra MT, Serra A, Sirigu P et al (1995) A histochemical and immunohistochemical study of certain defense mechanisms in the human lacrimal sac epithelium. Arch Histol Cytol 58(5):517–522
Paulsen FP, Thale AB, Hallmann UJ et al (2000) The cavernous body of the human efferent tear ducts: function in tear outflow mechanism. Invest Ophthalmol Vis Sci 41(5):965–970
Doane MG (1981) Blinking and the mechanics of the lacrimal drainage system. Ophthalmology 88(8):844–851
Ayub M, Thale AB, Hedderich J et al (2003) The cavernous body of the human efferent tear ducts contributes to regulation of tear outflow. Invest Ophthalmol Vis Sci 44(11):4900–4907
Roussos J, Bouzas A (1973) Attempted explanation with hormonal factors of the greater occurrence of chronic dacryocystitis in women than in men. Bull Mem Soc Fr Ophthalmol 86:96–99
Ali MJ, Schicht M, Paulsen F (2017) Qualitative hormonal profiling of the lacrimal drainage system: potential insights into the etiopathogenesis of primary acquired nasolacrimal duct obstruction. Ophthalmic Plast Reconstr Surg 33(5):381–388
Kashkouli MB, Sadeghipour A, Kaghazkanani R et al (2010) Pathogenesis of primary acquired nasolacrimal duct obstruction. Orbit 29(1):11–15
Lee-Wing MW, Ashenhurst ME (2001) Clinicopathologic analysis of 166 patients with primary acquired nasolacrimal duct obstruction. Ophthalmology 108(11):2038–2040
Frame NJ, Burkat CN (2009) Identifying an appropriate animal model for the nasolacrimal drainage system. Ophthal Plast Reconstr Surg 25(5):354–358
Paulsen FP, Foge M, Thale AB et al (2002) Animal model for the absorption of lipophilic substances from tear fluid by the epithelium of the nasolacrimal ducts. Invest Ophthalmol Vis Sci 43(10):3137–3143
Funding
This research was supported by the projects from the National Natural Science Foundation of China (No. 81501552). The sponsor had no role in the design or conduct of this research.
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All procedures performed in studies involving animals were in accordance with the ethical standards of the institution of Huazhong University of Science and Technology (HUST) at which the studies were conducted.
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Liu, R., Li, H., Ai, T. et al. Pathological changes of the nasolacrimal duct in rabbit models of chronic dacryocystitis: correlation with lacrimal endoscopic findings. Graefes Arch Clin Exp Ophthalmol 256, 2103–2112 (2018). https://doi.org/10.1007/s00417-018-4129-z
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DOI: https://doi.org/10.1007/s00417-018-4129-z