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Serum and aqueous xanthine oxidase levels, and mRNA expression in anterior lens epithelial cells in pseudoexfoliation

  • Glaucoma
  • Published:
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Abstract

Purpose

The aim of this study was to determine serum and aqueous xanthine oxidase (XO) levels, and mRNA expression in anterior lens epithelial cells in pseudoexfoliation (PEX).

Methods

In this prospective study, serum, aqueous and anterior lens capsules were taken from 21 patients with PEX and 23 normal subjects who had undergone routine cataract surgery. Serum and aqueous XO levels were analyzed using the colorimetric method. mRNA expression of XO in anterior lens epithelial cells was evaluated using reverse transcription polymerase chain reaction analysis.

Results

Serum XO levels (means ± standard deviations) were 207.0 ± 86.1 IU/mL and 240.6 ± 114.1 IU/mL in the normal and PEX groups, respectively (p = 0.310). Aqueous XO levels (means ± standard deviations) were 65.5 ± 54.3 IU/mL in the normal group and 130.5 ± 117.4 IU/mL in the PEX group (p = 0.028). There was a 2.9 fold decrease in mRNA expression in anterior lens epithelial cells of PEX, which is significantly lower than the normal group (p = 0.01).

Conclusions

Higher aqueous XO levels lacking associated different serum XO suggests higher oxidative stress in the aqueous. Higher aqueous XO levels in PEX with decreased mRNA expression in anterior lens epithelial cells indicate possible overexpression of XO in other structures related to the aqueous.

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Conflict of interest

All authors certify that they have NO affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript.

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Authors and Affiliations

  1. Department of Ophthalmology, Pamukkale University School of Medicine, Denizli, 20700, Turkey

    Huseyin Simavli

  2. Department of Biochemistry, Faculty of Medicine, Abant Izzet Baysal University, Bolu, 14080, Turkey

    Mehmet Tosun

  3. Eye Clinic, Bolu Izzet Baysal State Hospital, Bolu, 14000, Turkey

    Yasin Y. Bucak

  4. Faculty of Medicine, Department of Ophthalmology, Hacettepe University, Ankara, 06100, Turkey

    Mesut Erdurmus

  5. Department of Genetics, Faculty of Medicine, Abant Izzet Baysal University, Bolu, 14080, Turkey

    Zeynep Ocak

  6. Faculty of Medicine, Department of Ophthalmology, Duzce University, Duzce, 80100, Turkey

    Halil I. Onder

  7. School of Medicine, Department of Genetics, Turgut Özal University, Ankara, 06030, Turkey

    Muradiye Acar

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  1. Huseyin Simavli
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Correspondence to Huseyin Simavli.

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Simavli, H., Tosun, M., Bucak, Y.Y. et al. Serum and aqueous xanthine oxidase levels, and mRNA expression in anterior lens epithelial cells in pseudoexfoliation. Graefes Arch Clin Exp Ophthalmol 253, 1161–1167 (2015). https://doi.org/10.1007/s00417-015-3043-x

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  • DOI: https://doi.org/10.1007/s00417-015-3043-x

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