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Comparisons of microRNA expression profiles in vitreous humor between eyes with macular hole and eyes with proliferative diabetic retinopathy

  • Retinal Disorders
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Abstract

Purpose

MicroRNAs (miRNAs) are small noncoding RNAs which regulate the activities of target mRNAs. We compared the expression profiles of the miRNAs in the vitreous of eyes with macular hole (MH) to that in eyes with proliferative diabetic retinopathy (PDR).

Methods

Vitreous and whole blood samples were collected from four patients with MH and from four patients with PDR. We assayed for 168 miRNAs in the vitreous and serum samples by the microRNA PCR Panel method.

Results

The mean number of miRNAs expressed in the vitreous was 63 (55–69) in eyes with MH and 86 (65–117) in eyes with PDR. The mean number of miRNAs expressed in the serum was 162 (159–167) in the MH patients and 142 (115–160) in the PDR patients. Twenty-six miRNAs were expressed in the vitreous of both MH and PDR eyes. Although there was no significant difference in the levels of 20 of the 26 (73 %) miRNAs expressed in both MH and PDR eyes, six of 26 miRNAs (24 %) (hsa-miR-15a, hsa-miR320a, hsa-miR-320b, hsa-miR-93, hsa-miR-29a, and hsa-miR-423-5p) were expressed significantly more highly in PDR eyes. In addition, the mean fold changes of three miRNAs, hsa-miR-23a, hsa-miR-320a, and hsa-miR-320b, in the vitreous to serum were significantly higher in the PDR group than in the MH group.

Conclusions

The expression of several miRNAs related to angiogenesis and fibrosis was expressed significantly higher in the vitreous of eyes with PDR. Further studies are needed to understand the role played by the miRNAs in the biological function of the eye.

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Acknowledgements

The authors thank Nobuko Takahashi for her technical assistance and Professor Emeritus Duco Hamasaki of Bascom Palmer Eye Institute of the University of Miami for English editing.

Funding sources

This work was supported by Grant for Scientific Research from Kyorin University, Tokyo, Japan.

Conflicts of interest

No stated conflict of interest.

Author information

Authors and Affiliations

  1. Department of Ophthalmology, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo, 181-8611, Japan

    Kazunari Hirota, Hiroshi Keino, Makoto Inoue & Akito Hirakata

  2. Department of 3rd Internal Medicine, Kyorin University School of Medicine, Mitaka, Tokyo, Japan

    Hitoshi Ishida

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  1. Kazunari Hirota
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  2. Hiroshi Keino
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  3. Makoto Inoue
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Correspondence to Kazunari Hirota.

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Hirota, K., Keino, H., Inoue, M. et al. Comparisons of microRNA expression profiles in vitreous humor between eyes with macular hole and eyes with proliferative diabetic retinopathy. Graefes Arch Clin Exp Ophthalmol 253, 335–342 (2015). https://doi.org/10.1007/s00417-014-2692-5

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  • DOI: https://doi.org/10.1007/s00417-014-2692-5

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