Skip to main content

Advertisement

SpringerLink
Log in

Immunohistochemical evaluation of the extracellular matrix in trabecular meshwork in steroid-induced glaucoma

  • Glaucoma
  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

Background

To immunohistochemically examine the localization of the extracellular matrix (ECM) in the trabecular meshwork (TM) in steroid-induced glaucoma (SG) specimens.

Methods

We morphologically and immunohistochemically examined six trabecular tissues from three eyes with SG, two with primary open angle glaucoma (POAG), and one without glaucoma. For the morphological study, the ultra-microtome sections were observed using an electron microscope. For the light microscopic immunohistochemical analyses, frozen sections were stained by the avidin-biotin complex method using anti-type IV collagen, anti-type VI collagen, anti-heparan sulfate proteoglycan (HSPG), anti-fibronectin or anti-myocilin (MYOC) antibody.

Results

The morphological examinations revealed accumulations of basement membrane-like and fine fibrillar-like materials in the outer TM of SG specimen. Type IV collagen, HSPG and fibronectin antibodies in SG specimens showed a greater degree of staining in the outer TM in comparison to the POAG and non-glaucomatous specimens; in contrast, the other antibodies including the type VI collagen and MYOC, did not.

Conclusions

The localization of ECMs in the TM is different in SG in comparison to that in POAG patients.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Armaly MF (1963) Effect of corticosteroids on intraocular pressure and fluid dynamics. I. The effect of dexamethasone in the normal eye. Arch Ophthalmol 70:482–491

    PubMed  CAS  Google Scholar 

  2. Babizhayev MA, Brodskaya MW (1989) Fibronectin detection in drainage outflow system of human eyes in ageing and progression of open-angle glaucoma. Mech Ageing Dev 47:145–157

    Article  PubMed  CAS  Google Scholar 

  3. Clark AF, Steely HT, Dickerson JE Jr., English-Wright S, Stropki K, McCartney MD, Jacobson N, Shepard AR, Clark JI, Matsushima H, Peskind ER, Leverenz JB, Wilkinson CW, Swiderski RE, Fingert JH, Sheffield VC, Stone EM (2001) Glucocorticoid induction of the glaucoma gene MYOC in human and monkey trabecular meshwork cells and tissues. Invest Ophthalmol Vis Sci 42:1769–1780

    PubMed  CAS  Google Scholar 

  4. Clark AF, Wilson K, de Kater AW, Allingham RR, McCartney MD (1995) Dexamethasone-induced ocular hypertension in perfusion-cultured human eyes. Invest Ophthalmol Vis Sci 36:478–489

    PubMed  CAS  Google Scholar 

  5. Clark AF, Wilson K, McCartney MD, Miggans ST, Kunkle M, Howe W (1994) Glucocorticoid-induced formation of cross-linked actin networks in cultured human trabecular meshwork cells. Invest Ophthalmol Vis Sci 35:281–294

    PubMed  CAS  Google Scholar 

  6. Gould DB, Miceli-Libby L, Savinova OV, Torrado M, Tomarev SI, Smith RS, John SW (2004) Genetically increasing Myoc expression supports a necessary pathologic role of abnormal proteins in glaucoma. Mol Cell Biol 24:9019–9025

    Article  PubMed  CAS  Google Scholar 

  7. Hann CR, Springett MJ, Wang X, Johnson DH (2001) Ultrastructural localization of collagen IV, fibronectin, and laminin in the trabecular meshwork of normal and glaucomatous eyes. Ophthalmic Res 33:314–324

    Article  PubMed  CAS  Google Scholar 

  8. Johnson D, Gottanka J, Flugel C, Hoffmann F, Futa R, Lütjen-Drecoll E (1997) Ultrastructural changes in the trabecular meshwork of human eyes treated with corticosteroids. Arch Ophthalmol 115:375–383

    PubMed  CAS  Google Scholar 

  9. Lütjen-Drecoll E, May CA, Polansky JR, Johnson DH, Bloemendal H, Nguyen TD (1998) Localization of the stress proteins alpha B-crystallin and trabecular meshwork inducible glucocorticoid response protein in normal and glaucomatous trabecular meshwork. Invest Ophthalmol Vis Sci 39:517–525

    PubMed  Google Scholar 

  10. Nguyen TD, Chen P, Huang WD, Chen H, Johnson D, Polansky JR (1998) Gene structure and properties of TIGR, an olfactomedin-related glycoprotein cloned from glucocorticoid-induced trabecular meshwork cells. J Biol Chem 273:6341–6350

    Article  PubMed  CAS  Google Scholar 

  11. Polansky JR, Fauss DJ, Chen P, Chen H, Lütjen-Drecoll E, Johnson D, Kurtz RM, Ma ZD, Bloom E, Nguyen TD (1997) Cellular pharmacology and molecular biology of the trabecular meshwork inducible glucocorticoid response gene product. Ophthalmologica 211:126–139

    Article  PubMed  CAS  Google Scholar 

  12. Rodrigues MM, Katz SI, Foidart JM, Spaeth GL (1980) Collagen, factor VIII antigen, and immunoglobulins in the human aqueous drainage channels. Ophthalmology 87:337–345

    PubMed  CAS  Google Scholar 

  13. Rohen JW, Linner E, Witmer R (1973) Electron microscopic studies on the trabecular meshwork in two cases of corticosteroid-glaucoma. Exp Eye Res 17:19–31

    Article  PubMed  CAS  Google Scholar 

  14. Sakuragawa M, Iwata K (1979) Fine structure of the chamber angle in steroid glaucoma. Acta Soc Ophthalmol Jpn 83:1337–1353

    CAS  Google Scholar 

  15. Samples JR, Alexander JP, Acott TS (1993) Regulation of the levels of human trabecular matrix metalloproteinases and inhibitor by interleukin-1 and dexamethasone. Invest Ophthalmol Vis Sci 34:3386–3395

    PubMed  CAS  Google Scholar 

  16. Snyder RW, Stamer WD, Kramer TR, Seftor RE (1993) Corticosteroid treatment and trabecular meshwork proteases in cell and organ culture supernatants. Exp Eye Res 57:461–468

    Article  PubMed  CAS  Google Scholar 

  17. Steely HT, Browder SL, Julian MB, Miggans ST, Wilson KL, Clark AF (1992) The effects of dexamethasone on fibronectin expression in cultured human trabecular meshwork cells. Invest Ophthalmol Vis Sci 33:2242–2250

    PubMed  CAS  Google Scholar 

  18. Tawara A, Inomata H (1994) Distribution and characterization of sulfated proteoglycans in the trabecular tissue of goniodysgenetic glaucoma. Am J Ophthalmol 117:741–755

    PubMed  CAS  Google Scholar 

  19. Tawara A, Okada Y, Kubota T, Suzuki Y, Taniguchi F, Shirato S, Nguyen TD, Ohnishi Y (2000) Immunohistochemical localization of MYOC/TIGR protein in the trabecular tissue of normal and glaucomatous eyes. Curr Eye Res 21:934–943

    Article  PubMed  CAS  Google Scholar 

  20. Tawara A, Takahira K, Yamana T, Shigetou M, Inomata H (1993) Glaucoma induced by oral corticosteroid—Histological and histochemical studies of trabecular tissue. J Eye (Atarashii Ganka) 10:1181–1187

    Google Scholar 

  21. Ueda J, Wentz-Hunter K, Yue BY (2002) Distribution of myocilin and extracellular matrix components in the juxtacanalicular tissue of human eyes. Invest Ophthalmol Vis Sci 43:1068–1076

    PubMed  Google Scholar 

  22. Yun AJ, Murphy CG, Polansky JR, Newsome DA, Alvarado JA (1989) Proteins secreted by human trabecular cells. Glucocorticoid and other effects. Invest Ophthalmol Vis Sci 30:2012–2022

    PubMed  CAS  Google Scholar 

  23. Zhou L, Li Y, Yue BY (1998) Glucocorticoid effects on extracellular matrix proteins and integrins in bovine trabecular meshwork cells in relation to glaucoma. Int J Mol Med 1:339–346

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was upported by the Grants-in-aid for Scientific Research Grant No. 16591777 from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government, Japan.

Author information

Authors and Affiliations

  1. Department of Ophthalmology, University of Occupational & Environmental Health, Japan, 1–1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807–8555, Japan

    Akihiko Tawara, Norihiko Tou, Toshiaki Kubota, Yukinori Harada & Kiyomi Yokota

Authors
  1. Akihiko Tawara
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Norihiko Tou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Toshiaki Kubota
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yukinori Harada
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kiyomi Yokota
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Akihiko Tawara.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tawara, A., Tou, N., Kubota, T. et al. Immunohistochemical evaluation of the extracellular matrix in trabecular meshwork in steroid-induced glaucoma. Graefes Arch Clin Exp Ophthalmol 246, 1021–1028 (2008). https://doi.org/10.1007/s00417-008-0800-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00417-008-0800-0

Keywords

Search

Navigation