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Expression of CD18 on the neutrophils of patients with diabetic retinopathy

  • Clinical Investigation
  • Published:
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Abstract

Aim

To investigate the expression of CD18 on neutrophils from patients with various stages of diabetic retinopathy (DR) and to determine the possibility of using it as a marker of disease development.

Research design and methods

Levels of CD18 on neutrophils were measured by flow cytometry in 10 patients with DR stage 1, 14 patients in stages 2–4, 14 patients in stage 5 and 10 non-diabetic healthy subjects (control).

Results

Mean channel fluorescence (MCF) of CD18 on neutrophils from the control, stage 1, stages 2–4 and stage 5 groups were 1.36±0.17, 2.99±1.44, 3.13±1.10 and 4.11±1.62, respectively. The difference among them was significant (P=0.00). The highest was from the stage 5 group, then stages 2–4 and stage 1. The least was from the control group (trend test, P=0.00). This trend remained after adjusting for confounding variables. This trend was only significant in type 2 when subjects were divided according to diabetic type, even after adjusting for confounding factors. Multiple linear regression against the level of CD18 MCF by stage of DR and covariates revealed a significant and independent association with the stage of DR (β=0.33; P=0.01).

Conclusion

Elevated levels of CD18 on neutrophils were present in each stage of DR in type 2 diabetic patients. The more severe the disease, the higher the level was. CD18 may be a marker of the development of DR in type 2 diabetes.

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Acknowledgements

The authors would like to thank the two anonymous referees and Dr. Helen Li whose comments and suggestions greatly helped us to improve the presentation and quality of the paper. This work was partly supported by grant 2005 K14-G 6 from the Department of Science and Technology, Shaanxi Province, People’s Republic of China.

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Authors and Affiliations

  1. Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University and Eye Institute of PLA, Xian, 710032, People’s Republic of China

    Huping Song & Yannian Hui

  2. Department of Ophthalmology, Xian NO.4 Hospital, Xian, 710004, People’s Republic of China

    Lili Wang

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  1. Huping Song
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  2. Lili Wang
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  3. Yannian Hui
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Correspondence to Yannian Hui.

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Song, H., Wang, L. & Hui, Y. Expression of CD18 on the neutrophils of patients with diabetic retinopathy. Graefe's Arch Clin Exp Ophthalmol 245, 24–31 (2007). https://doi.org/10.1007/s00417-006-0379-2

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  • DOI: https://doi.org/10.1007/s00417-006-0379-2

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