Abstract
Purpose
To evaluate the histological changes in the retina after experimental vitreous substitution with various amounts of perfluorophenanthrene (PFPH).
Methods
Thirty-two rabbit eyes were mechanically vitrectomized and filled up with 0.8 cc or 0.2 cc highly purified PFPH. The substance remained for 1 week (4 eyes), 2 weeks (8 eyes), 4 weeks (10 eyes) or 8 weeks (10 eyes). Eight eyes underwent the same surgical procedure without PFPH exchange to serve as a control group. A histological comparison of corresponding areas in the center and in the periphery of the inferior retina ensued.
Results
After 2 weeks, nuclear drop-downs and irregularities of the outer plexiform layer and of both nuclear layers were observed centrally in the eyes with a 0.8 cc substitution. The changes proceeded to irregularities and cell loss of all retinal layers with focal areas of complete destruction of the retinal architecture after 8 weeks. In contrast, single nuclear drop-downs, wrinkling of the outer nuclear layer and cell loss in the photoreceptor layer were observed in the peripheral retina at the end of the observation period. In those eyes where 0.2 cc PFPH was exchanged nuclear drop-downs were found after 2 weeks, leading to focal thinning of the outer plexiform layer and irregularities of the outer nuclear layer after 4 weeks with an insignificant increase after 8 weeks. At this time these histological alterations were comparable with those that we observed after 2 weeks in the eyes with a 0.8 cc tamponade in a corresponding area.
Conclusions
Even high purification of PFPH does not prevent retinal damage. The different results in different areas after vitreous substitution with 0.2 cc and with 0.8 cc demonstrate that the high specific gravity of the substance may also play a role in the development of histological changes after extended tamponade.
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References
Binder S, Velikay M, Wedrich A, Stolba U, Datlinger P (1992) Die klinische Anwendung flüssiger Perfluorocarbone in der Netzhautchirurgie. Spektrum Augenheilkd 6:4–7
Blinder KJ, Peyman GA, Desai UR, Norman NC, Alturki W, Paris CL (1992) Vitreon, a short-term vitreoretinal tamponade. Br J Ophthalmol 76:525–528
Bottoni F, Sborgia M, Arpa P, De Casa N, Bertazzi E, Monticelli M (1993) Perfluorocarbon liquids as postoperative short-term vitreous substitutes in complicated retinal detachment. Graefes Arch Clin Exp Ophthalmol 231:619–628
Bryan JS, Friedman SM, Mames RN, Margo CE (1994) Experimental vitreous replacement with Perfluorotri-n-Propylamine. Arch Ophthalmol 112:1098–1102
Carroll BF, Peyman GA, Mehta NJ, Millsap CM, Greve MDJ, Dunlap WA (1994) Repair of retinal detachment associated with proliferative vitreoretinopathy using perfluoro-perhydrophenanthrene (Vitreon). Can J Ophthalmol 29:66–69
Chang S, Reppucci V, Zimmerman NJ, Heinemann MH, Coleman DJ (1981) Perfluorocarbon liquids in the management of traumatic retinal detachments. Ophthalmology 96:785–792
Chang S, Lincoff H, Zimmerman NJ, Fuchs W (1981) Giant retinal tears. Surgical techniques and results using Perfluorocarbon liquids. Arch Ophthalmol 107:761–766
Chang S, Zimmerman NJ, Iwamoto T, Ortiz R, Faris D (1987) Experimental vitreous replacement with Perfluorotributylamine. Am J Ophthalmol 103:29–37
Chang S, Ozmert E, Zimmerman NJ (1988) Intraoperative Perfluorocarbon liquids in the management of proliferative vitreoretinopathy. Am J Ophthalmol 106:668–674
Chang S, Sparrow JR, Iwamoto T, Gershbein A, Ross R, Ortiz R (1991) Experimental studies of tolerance to intravitreal Perfluoro-n-octane. Retina 11:367–374
Coll GE, Chang S, Sun J, Wieland MR, Berrocal MH (1995) Perfluorocarbon liquid in the management of retinal detachment with proliferative vitreoretinopathy. Ophthalmology 102:630–639
Eckardt C, Nicolai U, Winter M, Knop E (1991) Experimental intraocular tolerance to liquid Perfluorooctane and Perfluoropolyether. Retina 11:375–384
Fanous MM, Friedman SM (1992) Ciliary sulcus fixation of a dislocated posterior chamber intraocular lens using liquid perfluorophenanthrene. Ophthalmic Surg 23:551–552
Flores-Aguilar M, Munguia D, Loeb E, Crapotta JA, Vuong C, Shakiba S (1995) Intraocular tolerance of Perfluoro-octylbromide (Perflubron). Retina 15:3–13
Glaser BM, Carter JB, Kuppermann BD, Michels RG (1991) Perfluoro-octane in the treatment of giant retinal tears with proliferative vitreoretinopathy. Ophthalmology 98:1613–1621
Greve MDJ, Peyman GA, Mehta NJ, Millsap CM (1993) Use of Perfluoroperhydro-phenanthrene in the management of posteriorly dislocated crystalline and intraocular lenses. Ophthalmic Surg 24:593–597
Kirchhof B, Wong D, Van Meurs J, Hilgers RD, Macek M, Lois N, Schrage NF (2002) Use of perfluorohexyloctane as a long-term internal tamponade agent in complicated retinal detachment surgery. Am J Ophthalmol 133:95–101
Le Mer Y, Kroll P (1991) Die Verwendung von flüssigem Perfluorocarbon bei Riesenrissen. Klin Mb Augenheilkd 198:264–267
Lewis H, Sanchez G (1993) The use of perfluorocarbon liquids in the repositioning of posteriorly dislocated lenses. Ophthalmology 100:1055–1059
Lewis H, Blumenkranz MS, Chang S (1992) Treament of dislocated crystalline lens and retinal detachment with perfluorocarbon liquids. Retina 12:229–304
Liu KR, Peyman GA, Chen M, Cheng K (1991) Use of high density vitreous substitutes in the removal of posteriorly dislocated lenses or intraocular lenses. Ophthalmology 22:503–507
Mathis A, Pagot V, Gazague C, Malecaze F (1992) Giant retinal tears. Surgical techniques and results using perfluorodecaline and silicone oil tamponade. Retina 12(Suppl):7–10
Mathis A, Pagot V, Idder A, Malecaze F (1993) Utilisation de la perfluorodecaline au cours de la vitrectomie chez le diabetique. J Fr Ophtalmol 16(11):584–590
Miyamoto K, Refojo MF, Tolentino FI, Fournier GA, Albert DM (1984) Perfluoroether liquid as a long-term vitreous substitute. An experimental study. Retina 4:264–268
Nahib M, Peyman GA, Clark LC, Hoffman RE, Miceli M, Abou-Steit M (1989) Experimental evaluation of Perfluoro-phenanthrene as a high specific gravity vitreous substitute: a preliminary report. Ophthalmic Surg 20(4):286–293
Rowson NJ, Bacon AS, Rosen PH (1992) Perfluorocarbon heavy liquids in the management of posterior dislocation of the lens nucleus during phakoemulsification. Br J Ophthalmol 76:169–170
Schulman JA, Peyman GA, Blinder KJ (1993) Management of giant retinal tears with perfluoroperhydrophenanthrene (Vitreon). Jpn J Ophthalmol 37:70–77
Shapiro MJ, Resnik KI, Kim SH, Weinberg A (1991) Management of the dislocated crystalline lens with a Perfluorocarbon liquid. Am J Ophthalmol 112:401–405
Sparrow JR, Ortiz R, MacLeish PR, Chang S (1990) Fibroblast behaviour at aqueous interface with Perfluorocarbon, Silicone, and Fluorosilicone liquids. Invest Ophthalmol Vis Sci 31:638–646
Sparrow JR, Matthews GP, Iwamoto T, Ross R, Gershbein A, Chang S (1993) Retinal tolerance to intravitreal Perfluoro-ethylcyclohexane liquid in the rabbit. Retina 13:56–62
Velikay M, Wedrich A, Stolba U, Datlinger P, Li Y, Binder S (1993) Experimental long-term vitreous replacement with purified and non purified Perfluorodecaline. Am J Ophthalmol 116:565–570
Velikay M, Stolba U, Wedrich A, Li Y, Datlinger P, Binder S (1995) The effect of chemical stability and purification of Perfluorocarbon liquids in experimental extended-term vitreous substitution. Graefes Arch Clin Exp Ophthalmol 223(1):26–30
Verma LK, Peyman GA, Wafapor H, Greve MD, Millsap CM, Adile SL (1995) An analysis of posterior segment complications after vitrectomy using the perfluorocarbon perfluoroperhydrophenanthrene (Vitreon). Ophthalmic Surg 26:29–33
Wallace RT, McNamara JA, Brown G, Benson W, Belmont J, Goldberg R (1993) The use of perfluorophenanthrene in the removal of intravitreal lens fragments. Am J Ophthalmol 116:196–200
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The authors thank the Center of Biomedical Research Vienna (chairman: Prof. Dr. U. Losert) for the support they received.
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Stolba, U., Krepler, K., Velikay-Parel, M. et al. The effect of specific gravity of perfluorocarbon liquid on the retina after experimental vitreous substitution. Graefe's Arch Clin Exp Ophthalmol 242, 931–936 (2004). https://doi.org/10.1007/s00417-004-0916-9
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DOI: https://doi.org/10.1007/s00417-004-0916-9