Abstract
Background and purpose
Single-fibre electromyography (SF-EMG) is considered as the most sensitive test for the diagnosis of MG. However, previous studies had limitations, such as a retrospective design, non-consecutive sampling, incorporation bias or were performed in small or mixed populations. Our aims were to determine the diagnostic sensitivity and specificity of SF-EMG of the orbicularis oculi in OMG and the utility of this test in relation to patients’ clinical presentation.
Materials and methods
We studied 232 consecutive patients referred to the SF-EMG laboratory for a suspected OMG. Stimulated SF-EMG was performed on the orbicularis oculi muscle.
Results
OMG was diagnosed in 165 cases and other disorders (OD) in 67. SF-EMG showed a sensitivity of 0.79 (95% CI 0.73–0.85) and a specificity of 0.80 (95% CI 0.71–0.90). False negative results were associated with mild symptoms and with isolated diplopia. Comparison of the diagnostic yield among patients with different clinical presentations showed a similar diagnostic accuracy of SF-EMG in patients with ptosis and in patients with ptosis and diplopia, significantly higher than in patients with isolated diplopia (P < 0.0001). Twenty-two patients with OMG presenting with isolated ptosis or diplopia, who initially tested negative, were re-tested in relation to a worsening of their symptoms showing a positivisation in 91% of cases.
Conclusions
SF-EMG on the orbicularis oculi muscle is very sensitive in patients with ptosis. In contrast, in patients with isolated diplopia SF-EMG does not exclude OMG. Therefore, the interpretation of the results of the test should take into account the patients’ clinical presentation.
Similar content being viewed by others
References
Berrih-Aknin S, Le Panse R (2014) Myasthenia gravis: a comprehensive review of immune dysregulation and etiological mechanisms. J Autoimmun 52:90–100
Peeler CE, De Lott LB, Nagia L et al (2015) Clinical utility of acetylcholine receptor antibody testing in ocular myasthenia gravis. JAMA Neurol. https://doi.org/10.1001/jamaneurol.2015.1444
Meriggioli MN, Sanders DB (2009) Autoimmune myasthenia gravis: emerging clinical and biological heterogeneity. Lancet Neurol 8:475–490
Benatar M (2006) A systematic review of diagnostic studies in myasthenia gravis. Neuromuscul Disord. https://doi.org/10.1016/j.nmd.2006.05.006
Lo YL, Najjar RP, Teo KY et al (2017) A reappraisal of diagnostic tests for myasthenia gravis in a large Asian cohort. J Neurol Sci 376:153–158. https://doi.org/10.1016/j.jns.2017.03.016
Trontelj JV, Stålberg E (1992) Jitter measurement by axonal micro-stimulation. Guidelines and technical notes. Electroencephalogr Clin Neurophysiol Evoked Potentials 85:30–37. https://doi.org/10.1016/0168-5597(92)90098-V
Trontelj JV, Stålberg E (1992) Jitter measurement by axonal micro-stimulation. Guidelines and technical notes. Electroencephalogr Clin Neurophysiol Evoked Potentials. https://doi.org/10.1016/0168-5597(92)90098-V
Stålberg E, Trontelj JV, Mihelin M (1992) Electrical microstimulation with single-fiber electromyography: a useful method to study the physiology of the motor unit. J Clin Neurophysiol. https://doi.org/10.1097/00004691-199201000-00012
Oh SJ (2005) Single fiber electromyography: studies in healthy and diseased muscle, second edition. By Erik Stalberg and Joze V. Trontelji, Raven Press, 11985 Avenue of the Americas, New York, NY 10036, 1994, 303 pp., $79.00. Muscle Nerve. https://doi.org/10.1002/mus.880180718
DeLong ER, DeLong DM, Clarke-Pearson DL (1988) Comparing the areas under two or more correlated receiver operating characteristic curves: a nonparametric approach. Biometrics 44:837–845
Mercelis R, Merckaert V (2011) Diagnostic utility of stimulated single-fiber electromyography of the orbicularis oculi muscle in patients with suspected ocular myasthenia. Muscle Nerve 43:168–170. https://doi.org/10.1002/mus.21853
Padua L, Stalberg E, Lomonaco M et al (2000) SFEMG in ocular myasthenia gravis diagnosis. Clin Neurophysiol 111:1203–1207. https://doi.org/10.1016/S1388-2457(00)00307-2
-Canals J, Povedano M, Montero J, Pradas J (2003) Stimulated single-fiber EMG of the frontalis and orbicularis oculi muscles in ocular myasthenia gravis. Muscle Nerve 28:501–503. https://doi.org/10.1002/mus.10426
Morren JA, Levin KH, Shields RW (2016) Diagnostic accuracy of single fiber electromyography for myasthenia gravis in patients followed longitudinally. J Clin Neurophysiol 33:469–474. https://doi.org/10.1097/WNP.0000000000000285
Padua L, Caliandro P, Di Iasi G et al (2014) Reliability of SFEMG in diagnosing myasthenia gravis: sensitivity and specificity calculated on 100 prospective cases. Clin Neurophysiol 125:1270–1273. https://doi.org/10.1016/j.clinph.2013.11.005
Sanders DB, Howard JF (1986) AAEE Minimonograph #25: Single-fiber electromyography in myasthenia gravis. Muscle Nerve 9:809–819. https://doi.org/10.1002/mus.880090904
Farrugia ME, Jacob S, Sarrigiannis PG, Kennett RP (2009) Correlating extent of neuromuscular instability with acetylcholine receptor antibodies. Muscle Nerve 39:489–493. https://doi.org/10.1002/mus.21156
Jacob S, Viegas S, Leite MI et al (2012) Presence and pathogenic relevance of antibodies to clustered acetylcholine receptor in ocular and generalized myasthenia gravis. Arch Neurol 69:994–10005. https://doi.org/10.1001/archneurol.2012.437
Sanders DB, Arimura K, Cui LY et al (2019) Guidelines for single fiber EMG. Clin Neurophysiol. https://doi.org/10.1016/j.clinph.2019.04.005
Mittal MK, Barohn RJ, Pasnoor M et al (2011) Ocular myasthenia gravis in an academic neuro-ophthalmology clinic: clinical features and therapeutic response. J Clin Neuromuscul Dis 13(12):46–52. https://doi.org/10.1097/CND.0b013e31821c5634
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflicts of interest
The authors declare that they have no conflict of interest.
Rights and permissions
About this article
Cite this article
Giannoccaro, M.P., Di Stasi, V., Zanesini, C. et al. Sensitivity and specificity of single-fibre EMG in the diagnosis of ocular myasthenia varies accordingly to clinical presentation. J Neurol 267, 739–745 (2020). https://doi.org/10.1007/s00415-019-09631-3
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00415-019-09631-3