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Study of InDel genetic markers with forensic and ancestry informative interest in PALOP’s immigrant populations in Lisboa

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Abstract

The migratory phenomenon in Portugal has become one of the main factors for the genetic variability. In the last few years, a new class of autosomal insertion/deletion markers—InDel—has attracted interest in forensic genetics. Since there is no data for InDel markers of Portuguese-speaking African countries (PALOP) immigrants living in Lisboa, our aim is the characterization of those groups of individuals by typing them with at least 30 InDel markers and to compare different groups of individuals/populations. We studied 454 bloodstain samples belonging to immigrant individuals from Angola, Guinea-Bissau, and Mozambique. DNA extraction was performed with the Chelex® 100 method. After extraction, all samples were typed with the Investigator® DIPplex method. Through the obtained results, allelic frequencies show that all markers are at Hardy-Weinberg equilibrium, and we can confirm that those populations show significant genetic distances between themselves, between them, and the host Lisboa population. Because of this, they introduce genetic variability in Lisboa population.

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Authors and Affiliations

  1. Instituto Nacional de Medicina Legal e Ciências Forenses, Coimbra, Portugal

    Ana Inácio, Heloísa Afonso Costa, Cláudia Vieira da Silva, Teresa Ribeiro, Maria João Porto & António Amorim

  2. Departamento de Genética e Biotecnologia, Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal

    Ana Inácio & Gilberto Igrejas

  3. Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal

    Jorge Costa Santos

  4. Instituto Superior de Ciências da Saúde Egas Moniz, Monte de Caparica, Portugal

    Jorge Costa Santos & António Amorim

  5. Faculdade de Ciências, Universidade de Lisboa, Lisbon, Portugal

    António Amorim

  6. Centro de Ciências Forenses (CENCIFOR), Coimbra, Portugal

    Heloísa Afonso Costa, Cláudia Vieira da Silva, Teresa Ribeiro, Maria João Porto, Jorge Costa Santos & António Amorim

Authors
  1. Ana Inácio
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  2. Heloísa Afonso Costa
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  3. Cláudia Vieira da Silva
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  4. Teresa Ribeiro
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  5. Maria João Porto
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  6. Jorge Costa Santos
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  7. Gilberto Igrejas
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  8. António Amorim
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Correspondence to António Amorim.

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Inácio, A., Costa, H.A., da Silva, C.V. et al. Study of InDel genetic markers with forensic and ancestry informative interest in PALOP’s immigrant populations in Lisboa. Int J Legal Med 131, 657–660 (2017). https://doi.org/10.1007/s00414-016-1484-3

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  • DOI: https://doi.org/10.1007/s00414-016-1484-3

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