Abstract
The identification of putrefied bodies is a common task in forensic routine work. The deceased are usually identified by dental records, fingerprinting, or—in cases were no such data are available—DNA analysis. However, with progressive putrefaction, DNA integrity is rapidly decreasing. Genetic analysis may then be greatly impaired, if not impossible. The aim of our study was to establish an efficient procedure to successfully extract and amplify DNA from soft tissues of bodies in different stages of putrefaction. Soft tissues—unlike teeth or bones—usually allow the application of fast and easy-to-use extraction protocols. DNA was extracted from different tissues (aorta, kidney, liver, and skeletal muscle) taken at autopsy using a commercially available DNA extraction kit, and DNA quality and quantity were controlled by agarose gel electrophoresis and real-time polymerase chain reaction (PCR). Presence of mitochondrial DNA was tested using a highly sensitive duplex PCR. Short tandem repeat analysis was done using the AmpFlSTR Identifiler kit. Additionally, mitochondrial DNA sequencing was performed. After DNA extraction from at least two different tissues—preferably the kidney and the aorta—with the extraction kit based on the Nucleobond method, a successful amplification of at least eight loci was possible in 17 out of 18 cases, and 12 or more loci could be amplified in 15 cases.
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References
Interpol (2009) Disaster victim identification guide. http://www.interpol.int/disastervictim/guide/guide.pdf
Butler J (2007) Short tandem repeat typing technologies used in human identity testing. Biotechniques 43:ii–v
Tsokos M, Lessig R, Grundmann C, Benthaus S, Peschel O (2006) Experiences in tsunami victim identification. Int J Leg Med 120:185–187
Biesecker LG, Bailey-Wilson JE, Ballantyne J, Baum H, Bieber FR, Brenner C, Budowle B, Butler JM, Carmody G, Conneally PM, Duceman B, Eisenberg A, Forman L, Kidd KK, Leclair B, Niezgoda S, Parsons TJ, Pugh E, Shaler R, Sherry ST, Sozer A, Walsh A (2005) Epidemiology. DNA identifications after the 9/11 World Trade Center attack. Science 18:1122–1123
Mundorff AZ, Bartelink EJ, Mar-Cash E (2009) DNA preservation in skeletal elements from the World Trade Center disaster: recommendations for mass fatality management. J Forensic Sci 54:739–745
Meyer HJ (2003) The Kaprun cable car fire disaster—aspects of forensic organisation following a mass fatality with 155 victims. Forensic Sci Int 138:1–7
Staiti N, Di Martino D, Saravo L (2004) A novel approach in personal identification from tissue samples undergone different processes through STR typing. Forensic Sci Int 146:S171–S173
Prinz M, Carracedo A, Mayr WR, Morling N, Parsons TJ, Sajantila A, Scheithauer R, Schmitter H, Schneider PM (2007) DNA Commission of the International Society for Forensic Genetics (ISFG): recommendations regarding the role of forensic genetics for disaster victim identification (DVI). Forensic Sci Int Genet 1:3–12
von Wurmb-Schwark N, Ringleb A, Gebühr M, Simeoni E (2005) Genetic analysis of modern and historical burned human remains. Anthropol Anz 63:1–12
von Wurmb-Schwark N, Schwark T, Harbeck M, Oehmichen M (2004) Amplification of mtDNA and nuclear DNA in a duplex PCR to evaluate the DNA quality of anthropological and forensic samples prior to STR typing. Leg Med 2:80–88
Pfeiffer H, Forster P, Ortmann C, Brinkmann B (2001) The results of an mtDNA study of 1,200 inhabitants of a German village in comparison to other Caucasian databases and its relevance for forensic casework. Int J Leg Med 114:169–172
Motani H, Sakurada K, Akutsu T, Ikegaya H, Hayakawa M, Sato Y, Yahima D, Sato K, Kobayashi K, Iwase H (2006) Usefulness of dura mater in providing DNA samples for identifying cadavers. J Forensic Sci 51:1–5
Köchl S, Niederstätter H, Parson W (2005) DNA extraction and quantitation of forensic samples using the phenol-chloroform method and real-time PCR. Methods Mol Biol 297:13–30
Hoff-Olson P, Mevag B, Staastrom E, Hovde B, Egeland T, Olaisen B (1999) Extraction of DNA from decomposed human tissue: an evaluation of five extraction methods for short tandem repeat typing. Forensic Sci Int 105:171–183
Sato Y, Motani H, Inoue H, Hayakawa M, Yajima D, Nagasawa S, Kobayashi K, Saot K, Otsuka K, Iwase H (2009) Multiplex STR typing of aortic tissue from unidentified cadavers. Leg Med 11:455–457
Shintani-Sishida K, Harada K, Nakajima M, Yoshid K (2010) Usefulness of blood vessels as a DNA source for PCR-based genotyping based on two cases of corpse dismemberment. Leg Med 12:8–12
Alonso A, Martin P, Albarrán C, Garcia P, Fernandez de Simon L, Jesús Iturralde M, Fernández-Rodriguez A, Atienza I, Capilla J, García-Hirschfeld J, Martinez P, Vallejo G, García O, García E, Real P, Alvarez D, León A, Sancho M (2005) Challenges of DNA profiling in mass disaster investigations. Croat Med J 46:540–548
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Schwark, T., Heinrich, A. & von Wurmb-Schwark, N. Genetic identification of highly putrefied bodies using DNA from soft tissues. Int J Legal Med 125, 891–894 (2011). https://doi.org/10.1007/s00414-010-0537-2
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DOI: https://doi.org/10.1007/s00414-010-0537-2