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A novel and an effective analytical approach for the LC-MS determination of ethyl glucuronide and ethyl sulfate in urine

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Abstract

An alternative liquid chromatography–mass spectrometry (LC-MS) method based on no discharge (ND) atmospheric pressure chemical ionization (APCI) was developed for the simultaneous determination of ethyl glucuronide (EtG) and ethyl sulfate (EtS) in urine in negative ion conditions. Abundant [M-H]− species of EtG and EtS were obtained, allowing to reach limits of quantification (0.1 µg/ml for both analytes), accuracy, and precision comparable to those proposed in the literature. Additionally, the LC-ND–APCI-MS method proved to be reliable, requiring little maintenance even when high throughput analyses (i.e., 6,000 samples per year) were required.

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Authors and Affiliations

  1. Forensic Toxicology and Antidoping, University Hospital of Padova, Via Falloppio 50, 35121, Padova, Italy

    Donata Favretto, Alessandro Nalesso, Giampietro Frison, Guido Viel & Santo Davide Ferrara

  2. Consiglio Nazionale delle Ricerche–Institute of Molecular Science and Technologies, Corso Stati Uniti 4, 35100, Padova, Italy

    Pietro Traldi

Authors
  1. Donata Favretto
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  2. Alessandro Nalesso
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  3. Giampietro Frison
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  4. Guido Viel
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  5. Pietro Traldi
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  6. Santo Davide Ferrara
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Corresponding author

Correspondence to Santo Davide Ferrara.

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Favretto, D., Nalesso, A., Frison, G. et al. A novel and an effective analytical approach for the LC-MS determination of ethyl glucuronide and ethyl sulfate in urine. Int J Legal Med 124, 161–164 (2010). https://doi.org/10.1007/s00414-009-0376-1

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  • DOI: https://doi.org/10.1007/s00414-009-0376-1

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