Abstract
The five X-chromosomal short tandem repeats (STRs) GATA172D05, HPRTB, DXS8377, DXS101 and HumARA were analyzed in 150 males and 150 females from Korea. Markers were amplified in a quadruplex and a monoplex PCR reaction with fluorescently labeled primers. For accurate and reproducible STR typing, sequenced allelic ladders were constructed and a Genotyper macro was programmed. Some differences were found on comparing the allele frequencies of Koreans with those of other populations in DXS8377, DXS101 and HumARA. The forensic efficiency parameters showed that the five X-linked STRs are highly informative for forensic application in Koreans.
Similar content being viewed by others
References
Urquhart A, Kimpton CP, Downes TJ, Gill P (1994) Variation in short tandem repeat sequences: a survey of twelve microsatellite loci for use as forensic identification markers. Int J Legal Med 107:13–20
Brinkmann B (1998) Overview of PCR-based systems in identity testing. Methods Mol Biol 98:105–119
Jobling MA, Pandya A, Tyler-Smith C (1997) The Y chromosome in forensic analysis and paternity testing. Int J Legal Med 110:118–124
Szibor R, Krawczak M, Hering S, Edelmann J, Kuhlisch E, Krause D (2003) Use of X-linked markers for forensic purposes. Int J Legal Med 117:67–74
Edwards A, Civitello A, Hammond HA, Caskey CT (1991) DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am J Hum Genet 49:746–756
Hearne CM, Todd JA (1991) Tetranucleotide repeat polymorphism at the HPRT locus. Nucleic Acids Res 19:5450
Edwards A, Hammond HA, Jin L, Caskey CT, Chakraborty R (1992) Genetic variation at five trimeric and tetrameric tandem repeat loci in four human population groups. Genomics 12:241–253
Kishida T, Wang W, Fukuda M, Tamaki Y (1997) Duplex PCR of the Y-27H39 and HPRT loci with reference to Japanese population data on the HPRT locus. Nippon Hoigaku Zasshi 51:67–69
Mertens G, Gielis M, Mommers N et al. (1999) Mutation of the repeat number of the HPRTB locus and structure of rare intermediate alleles. Int J Legal Med 112:192–194
Szibor R, Lautsch S, Plate I, Beck N (2000) Population data on the X chromosome short tandem repeat locus HumHPRTB in two regions of Germany. J Forensic Sci 45:231–233
Edelmann J, Hering S, Michael M et al. (2001) 16 X-chromosome STR loci frequency data from a German population. Forensic Sci Int 124:215–218
Chen MY, Wu FC, Meng LM, Cheng SM, Chao CH, Pu CE (2002) Population data on X chromosome short tandem repeat loci HPRTB and AR in Taiwan. Forensic Sci Int 126:171–172
Zarrabeitia MT, Amigo T, Sanudo C, Zarrabeitia A, Gonzalez-Lamuno D, Riancho JA (2002) A new pentaplex system to study short tandem repeat markers of forensic interest on X chromosome. Forensic Sci Int 129:85–89
Sleddens HF, Oostra BA, Brinkmann AO, Trapmann J (1992) Trinucleotide repeat polymorphism in the androgen receptor gene (AR). Nucleic Acids Res 20:1427
Kishida T, Tamaki Y (1997) Japanese population data on X-chromosomal STR locus AR. Nippon Hoigaku Zasshi 51:376–379
Desmarais D, Zhong Y, Chakraborty R, Perreault C, Busque L (1998) Development of a highly polymorphic STR marker for identity testing purposes at the human androgen receptor gene (HUMARA). J Forensic Sci 43:1046–1049
Edelmann J, Deichsel D, Hering S, Plate I, Szibor R (2002) Sequence variation and allele nomenclature for the X-linked STRs DXS9895, DXS8378, DXS7132, DXS6800, DXS7133, GATA172D05, DXS7423 and DXS8377. Forensic Sci Int 129:99–103
Zarrabeitia MT, Amigo T, Sanudo C, Pancorbo MM de, Riancho JA (2002) Sequence structure and population date of two X-linked markers: DXS7423 and DXS8377. Int J Legal Med 116:368–371
Wiegand P, Berger B, Edelmann J, Parson W (2003) Population genetic comparisons of three X-chromosomal STRs. Int J Legal Med 117:62–65
Allen RC, Belmont JW (1993) Trinucleotide repeat polymorphism at DXS101. Hum Mol Genet 2:1508
Edelmann J, Szibor R (2001) DXS101: a highly polymorphic X-linked STR. Int J Legal Med 114:301–304
Bär W, Brinkmann B, Budowle B et al. (1997) DNA recommendations. Further report of the DNA Commission of the ISFH regarding the use of short tandem repeat systems. International Society for Forensic Haemogenetics. Int J Legal Med 110:175–176
Nei M, Roychoudhury AK (1974) Sampling variances of heterozygosity and genetic distance. Genetics 76:379–390
Botstein D, White RI, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331
Szibor R, Edelmann J, Zarrabeitia MT, Riancho JA (2003) Sequence structure and population data of the X-linked markers DXS7423 and DXS8377—clarification of conflicting statements published by two working groups. Forensic Sci Int 134:72–73
Acknowledgements
This work was supported by a grant from the Korea Science and Engineering Foundation through the Biometrics Engineering Research Center at Yonsei University.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Shin, KJ., Kwon, BK., Lee, SS. et al. Five highly informative X-chromosomal STRs in Koreans. Int J Legal Med 118, 37–40 (2004). https://doi.org/10.1007/s00414-003-0415-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00414-003-0415-2