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Spanish population data and forensic usefulness of a novel Y-STR set (DYS437, DYS438, DYS439, DYS460, DYS461, GATA A10, GATA C4, GATA H4)

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Abstract

DNA typing of 8 recently described STRs on the Y chromosome was carried out by means of 2 multiplex amplification reactions for 134 unrelated males from Cantabria, a region in northern Spain. Multiplex 1 included loci DYS460 (GATA A7.1), GATA A10, GATA H4 and DYS439; multiplex 2 included DYS461 (GATA A7.2), GATA C4, DYS437 and DYS438. Haplotype diversity was found to be 99.36%, similar to that obtained with the standard 9-STR set ("minimal haplotype") of the European Y-user group (99.35%). The 13-locus haplotype resulting from the combination of the standard minimal haplotype and the 4-locus multiplex 1 showed a 99.89% diversity. Further inclusion of the 4 loci in multiplex 2 resulted in a haplotype diversity of 99.93%. The combination of the "minimal haplotype" and the multiplex 1 in the present study may be an efficient way of increasing the power of discrimination in forensic cases.

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References

  1. Roewer L, Krawczak M, Willuweit S et al. (2001) Online reference database of European Y-chromosomal short tandem repeat (STR) haplotypes. Forensic Sci Int 118:106–113

    CAS  PubMed  Google Scholar 

  2. Ayub Q, Mohyuddin A, Qamar R, Mazhar K, Zerjal T, Mehdi SQ, Tyler-Smith C (2000) Identification and characterisation of novel Y-chromosomal microsatellites from sequence database information. Nucleic Acids Res 28:e8

    CAS  PubMed  Google Scholar 

  3. White PS, Tatum OL, Deaven LL, Longmire JL (1999) New, male-specific microsatellite markers from the human Y chromosome. Genomics 57:433–437

    CAS  PubMed  Google Scholar 

  4. González-Neira A, Elmoznino M, Lareu MV, Sánchez-Diz P, Gusmão L, Prinz M, Carracedo A (2001) Sequence structure of 12 novel Y chromosome microsatellites and PCR amplification strategies. Forensic Sci Int 122:19–26

    PubMed  Google Scholar 

  5. Sánchez-Diz P, Gusmão L, Beleza S et al. (2003) Results of the GEP-ISFG collaborative study on two Y-STR tetraplexes: GEPY I (DYS461, GATA C4, DYS437 and DYS438) and GEPY II (DYS460, GATA A10, GATA H4, DYS439). Forensic Sci Int, in press

  6. Beleza S, Alves C, González-Neira A, Lareu M, Amorim A, Carracedo A, Gusmão L (2003) Extending STR markers in Y chromosome haplotypes. Int J Legal Med 117:27–33

    Google Scholar 

  7. Schneider PM, Meuser S, Waiyawuth W, Seo Y, Rittner C (1998) Tandem repeat structure of the duplicated Y-chromosomal STR locus DYS385 and frequency studies in the German and three Asian populations. Forensic Sci Int 97:61–70

    CAS  PubMed  Google Scholar 

  8. Gusmão L, Gonzalez-Neira A, Pestoni C, Brion M, Lareu MV, Carracedo A (1999) Robustness of the Y STRs DYS19, DYS389 I and II, DYS390 and DYS393: optimization of a PCR pentaplex. Forensic Sci Int 106:163–172

    CAS  PubMed  Google Scholar 

  9. Gusmão L, Gonzalez-Neira A, Sanchez-Diz P, Lareu MV, Amorim A, Carracedo A (2000) Alternative primers for DYS391 typing: advantages of their application to forensic genetics. Forensic Sci Int 112:49–57

    PubMed  Google Scholar 

  10. Kayser M, Caglia A, Corach D et al. (1997) Evaluation of Y-chromosomal STRs: a multicenter study. Int J Legal Med 110:125–133

    CAS  PubMed  Google Scholar 

  11. Zarrabeitia MT, Riancho JA, Sánchez-Diz P, Sánchez-Velasco P (2001) 7-Locus Y chromosome haplotype profiling in a northern Spain population. Forensic Sci Int 123:78–80

    Article  CAS  PubMed  Google Scholar 

  12. Gusmão L, Alves C, Beleza S, Amorim A (2002) Forensic evaluation and population data on the new Y-STRs DYS434, DYS437, DYS438, DYS439 and GATA A10. Int J Legal Med 116:139–147

    PubMed  Google Scholar 

  13. Gusmão L, Gonzalez-Neira A, Lareu MV, Costa S, Amorim A, Carracedo A (2002) Chimpanzee homologous of human Y specific STRs. A comparative study and a proposal for nomenclature. Forensic Sci Int 126:129–136

    Article  PubMed  Google Scholar 

  14. Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York

  15. Hou YP, Zhang J, Li YB, Wu J, Zhang SZ, Prinz M (2001) Allele sequences of six new Y-STR loci and haplotypes in the Chinese Han population. Forensic Sci Int 118:147–152

    CAS  PubMed  Google Scholar 

  16. Bosch E, Lee AC, Calafell F, Arroyo E, Henneman P, Knijff P de, Jobling MA (2002) High resolution Y chromosome typing: 19 STRs amplified in three multiplex reactions. Forensic Sci Int 125:42–51

    CAS  PubMed  Google Scholar 

  17. Tsai LC, Yuen TY, Hsieh HM, Lin M, Tzeng CH, Huang NE, Linacre A, Lee JCI (2002) Haplotype frequencies of nine Y-chromosome STR loci in the Taiwanese Han population. Int J Legal Med 116:179–183

    PubMed  Google Scholar 

  18. Shin DJ, Jin HJ, Kwak KD et al. (2001) Y-chromosome multiplexes and their potential for DNA profiling in Koreans. Int J Legal Med 115:109–117

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgement

Supported in part by a grant from Fundación Marqués de Valdecilla.

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Authors and Affiliations

  1. Unidad de Medicina Legal, Facultad de Medicina, Universidad de Cantabria C/Herrera Oria, s/n, 39011 , Santander, Spain

    María T. Zarrabeitia & Carolina Sañudo

  2. Universidad de Cantabria, Departamento de Medicina Interna, Santander, Spain

    José A. Riancho

  3. Universidad de Santiago, Instituto de Medicina Legal, Santiago de Compostela, Spain

    María V. Lareu & Angel Carracedo

  4. Universidad do Porto, Instituto de Patologia e Imunologia Molecular, Porto, Portugal

    Leonor Gusmão & António Amorim

Authors
  1. María T. Zarrabeitia
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  2. José A. Riancho
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  3. Leonor Gusmão
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  4. María V. Lareu
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  5. Carolina Sañudo
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  6. António Amorim
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  7. Angel Carracedo
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Corresponding author

Correspondence to María T. Zarrabeitia.

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Zarrabeitia, M.T., Riancho, J.A., Gusmão, L. et al. Spanish population data and forensic usefulness of a novel Y-STR set (DYS437, DYS438, DYS439, DYS460, DYS461, GATA A10, GATA C4, GATA H4). Int J Legal Med 117, 306–311 (2003). https://doi.org/10.1007/s00414-003-0393-4

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  • DOI: https://doi.org/10.1007/s00414-003-0393-4

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